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Arbuscular Mycorrhizal Fungi in Roots and Rhizosphere of Black Rice in Terrace Fields of North-East India

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Morphological assessment of arbuscular mycorrhizal (AM) fungal diversity in rhizosphere soils may not reflect the AM fungal species colonizing the roots. Recently, DNA-based molecular approaches offer a wide range of advantages in understanding the AM fungal abundance both in soil and root systems. In this study, the community composition of native AM fungi in the soil and roots of aromatic black rice (Oryza sativa L. cv. Chakhao amubi) during the flowering stage in the terrace farming system was evaluated. AM fungal spore density ranged between 72 and 110 spores per 100 g shade-dried soil and varied significantly with rice terraces. All examined root fragments were colonized by AM fungi. However, the root length containing different AM fungal structures did not vary significantly compared to total root length colonization. The genomic DNA from rice roots was amplified with AM fungal specific primers using nested PCR and sequenced thereafter. Results of 18S rDNA partial region sequences revealed the presence of seven AM fungal species in roots, whereas in rhizosphere soils ten AM fungal spore morphotypes were recorded. Such dissimilarity in species richness of AMF between rhizosphere soil and the roots implied that some indigenous AMF species were adapted to specific soil environments that could colonize the paddy crop roots and suggests that the conditions can be suitable for some AMF species which have an important function in traditional terrace rice cultivation of hilly tracts in North-East India.

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Acknowledgements

The authors gratefully acknowledge the financial assistance from Network Project on Application of Microorganisms in Agriculture and Allied Sector (AMAAS), sponsored by Indian Council of Agricultural Research, New Delhi.

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Authors and Affiliations

  1. Department of Botany, Manipur University, Canchipur, Imphal, Manipur, 795 003, India

    Kannaiah Surendirakumar & Radha Raman Pandey

  2. Root and Soil Biology Laboratory, Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Thangavelu Muthukumar

Authors
  1. Kannaiah Surendirakumar
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  2. Radha Raman Pandey
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  3. Thangavelu Muthukumar
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Correspondence to Radha Raman Pandey.

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Significance Statement

This study reveals the AM fungal colonization patterns differing with altitudes and species diversity of AM fungi in both rhizosphere soil and colonized roots of aromatic black rice cv. Chakhao amubi under terrace farming system of NE India.

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Surendirakumar, K., Pandey, R.R. & Muthukumar, T. Arbuscular Mycorrhizal Fungi in Roots and Rhizosphere of Black Rice in Terrace Fields of North-East India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 277–287 (2021). https://doi.org/10.1007/s40011-020-01221-y

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  • DOI: https://doi.org/10.1007/s40011-020-01221-y

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