Epigenetic Modifier Based Enhancement of Piperine Production in Endophytic Diaporthe sp. PF20
- 20 Downloads
Biodiversity and metabolite richness of endophytic fungi are highly fascinating. Some of them have even been reported to have the shared biosynthetic basis for the production of plant specific metabolites. However, only limited reports are there on enhancement of production of plant specific metabolites from endophytic fungi. In the study, endophytic Diaporthe sp. PF20 from Piper nigrum L. was identified to have the ability to produce piperine by liquid chromatography tandem mass spectroscopy. The isolate PF20 was further subjected to epigenetic treatment along with previously characterized piperine producing Colletotrichum sp. and Mycosphaerella sp. from the same plant. Very interestingly, use of histone deacetylase inhibitor suberohydroxamic acid enhanced the piperine production in PF20. Here, the epigenetic modulator mediated enhancement of phytochemical biosynthetic potential of endophytic fungi is novel in its approach. Hence the results of the study open up new avenues to maintain the biosynthetic competency of endophytic fungi, which is highly challenging. Even though piperine production has previously been reported from endophytic fungi, epigenetic modulator mediated multiplexing of this property as observed for PF20 is highly attractive. This is because, only this organism was found to be susceptible to epigenetic modulation based piperine enhancement among the selected isolates.
KeywordsDiaporthe sp. Endophytic fungi Epigenetic modification Piperine Piper nigrum
The present study was supported by Department of Biotechnology (DBT), Government of India under DBT-RGYI and DBT-MSUB support scheme (BT/PR4800/INF/22/152/2012 dated 23.03.2012) and Kerala State Council Science Technology and Environment (KSCSTE) under KSCSTE-SARD Programme. The authors also acknowledge Prof. C.T. Aravindakumar, Hon. Director and Mr. Dineep D., Scientific Assistant of the Inter-University Instrumentation Centre, Mahatma Gandhi University, Kottayam for the help and support for the LC–MS/MS analysis. They also acknowledge Dr. Jayachandran K, Associate Professor, School of Biosciences, Mahatma Gandhi University, PD Hills PO, Kottayam, India and Principal Investigator, Kerala Biotechnology Commission—Young Investigators Programme in Biotechnology (YIPB) programme for the help and support in performing the HPLC analysis.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest to publish this manuscript.
- 4.Zhao LF, Xu YJ, Ma ZQ et al (2013) Colonization and plant growth promoting characterization of endophytic Pseudomonas chlororaphis strain Zong1 isolated from Sophora alopecuroides root nodules. Braz J Microbiol 44:623–631. https://doi.org/10.1590/S1517-83822013000200043 PubMedPubMedCentralGoogle Scholar
- 9.Cichewicz R (2012) Epigenetic regulation of secondary metabolite biosynthetic genes in fungi. In: Witzany G (eds) Biocommuniaction of fungi. Springer, Dordrecht, pp 57–69Google Scholar
- 13.Khajuria A, Thusa N, Zusthi U, Bedi KL (1997) Estimation of piperine in commercial ayurvedic formulations. J Indian Drugs 34:557–563Google Scholar
- 19.Sharma VK, Kumar J, Singh DK, Mishra A, Gond SK, Verma SK, Kumar A, Singh G, Kharwar RN (2017) Induction of cryptic metabolite production through epigenetic tailoring in Colletotrichum gloeosporioides isolated from Syzygium cumini. In: Azevedo JL, Quecine MC (eds) Diversity and benefits of microorganisms from the tropics Sringer, pp 169–18 ( https://doi.org/10.1007/978-3-319-55804-2) ISBN 978-3-319-55803-5
- 22.Chithra S, Jasim B, Mathew J, Radhakrishnan EK (2017) Endophytic Phomopsis sp. colonization in Oryza sativa was found to result in plant growth promotion and piperine production 437–446. https://doi.org/10.1111/ppl.12556