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Recombinant Partial Conglutinin of Buffalo and Nilgai In Vitro Can Mimic the Functions of Native Conglutinin In Vivo

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Conglutinin, a high molecular weight protein detected in bovine serum, belongs to the family of innate immunity markers called collectins. It plays a key role as antimicrobial agent against all kinds of micro-organisms by binding to sugar moieties present on them. In the present study, partial recombinant conglutinin consisting neck and carbohydrate recognition domain of buffalo and nilgai was expressed in prokaryotic system and found to be of 27 kDa through SDS-PAGE and western blotting using antiserum raised against buffalo conglutinin. In the presence of calcium, protein showed high binding activity towards mannan coated to microtitre plate but in contrast, binding activity towards lipopolysaccharide was calcium independent, which needs to be explored further. The partial protein was unable to inhibit haemagglutination by Newcastle disease virus; this could be attributed to lack of complete protein, as complete protein forms tetramer which can cross link the virus. The protein was also found to reduce bovine herpes virus-1 titre propagated in MDBK cells. This paves path to check the effect of complete conglutinin protein on different enveloped viruses, which will provide insight into protein’s importance in innate immunity. Since the concentration of serum conglutinin is found to be hereditary dependent, this could be also helpful in better parentage selection.

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Acknowledgements

The authors are thankful to the Director, Indian Veterinary Research Institute, Izatnagar for providing necessary facilities to carry out the work.

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Authors and Affiliations

  1. Department of Veterinary Biochemistry, Veterinary College, Gadag, Karnataka, India

    D. Ramesh

  2. Centre for Wildlife, Indian Veterinary Research Institute, Izzatnagar, India

    S. Chandra Mohan & Anil Kumar Sharma

  3. Division of Biochemistry, Indian Veterinary Research Institute, Izzatnagar, India

    Mohini Saini & Sasmita Barik

  4. Department of Physiology and Biochemistry, Veterinary College, Pookode, Kerala, India

    M. Shynu

  5. Division of Animal Nutrition, Indian Veterinary Research Institute, Izzatnagar, India

    Asit Das

  6. Division of Biological Products, Indian Veterinary Research Institute, Izzatnagar, India

    Vinod Kumar Chaturvedi

  7. Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izzatnagar, India

    Praveen Kumar Gupta

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  1. D. Ramesh
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  2. S. Chandra Mohan
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  3. Mohini Saini
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  4. Sasmita Barik
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  5. M. Shynu
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  6. Asit Das
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  7. Anil Kumar Sharma
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  8. Vinod Kumar Chaturvedi
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  9. Praveen Kumar Gupta
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Correspondence to D. Ramesh.

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Significance statement

The present work signifies the importance of the conglutinin as an innate immunity marker in ruminants. The recombinant conglutinin can be used as antigen to standardize quantitative sandwich ELISA to quantitate native conglutinin. As the concentration of native serum conglutinin is hereditary dependent and also known to increase survivability of newborn kids, quantitative ELISA will be helpful in selecting parents with strong innate immune system.

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Ramesh, D., Chandra Mohan, S., Saini, M. et al. Recombinant Partial Conglutinin of Buffalo and Nilgai In Vitro Can Mimic the Functions of Native Conglutinin In Vivo. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 639–648 (2019). https://doi.org/10.1007/s40011-018-0979-8

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  • DOI: https://doi.org/10.1007/s40011-018-0979-8

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