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Chlorophyll Binding Ability of Non-chloroplastic DUF538 Protein Superfamily in Plants

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The type-1 water soluble chlorophyll binding proteins (WSCP1) have been generally known as chlorophyll extractors and transporters from the thylakoid membrane to the chloroplast envelope, where the membrane bound chlorophyllase catabolizes the chlorophyll. Despite the type-2 WSCP, WSCP1 has been known to be located in the chloroplasts of the green plants. In the present study, the non-chloroplastic protein superfamily containing domain of unknown function 538 (DUF538) as functional homologue of type-1 WSCP has been identified in plants. The structural similarities/differences and the cellular locations of Celosia cristata DUF538 and Chenopodium album WSCP1 were predicted by using computational tools and the chlorophyll binding abilities of their purified maltose binding protein-fused forms were estimated by maltose binding affinity method. It was predicted that despite CaWSCP1, CcDUF538 is a non-chloroplastic protein. The chlorophyll binding abilities of the recombinant fusion forms of test WSCP1 and DUF538 were estimated to be about 58 and 56%, respectively. Considering DUF538 as stress-induced protein, it was speculated that they may form complex with chlorophyll molecules or their hydrolyzed products out of chloroplasts to proceed the chlorophyll breakdown and nitrogen/carbon recycling in stress-challenged plants.

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Abbreviations

WSCP:

Water soluble chlorophyll binding proteins

DUF:

Domain of unknown function

BPI:

Bactericidal permeability increasing

IPTG:

Isopropyl-1-thio-β-d-galactoside

NBT:

Nitro blue tetrazolium

BCIP:

5-bromo-1-chloro-3-indolyl phosphate

DMF:

Dimethyl formamide

Pchl:

Proto chlorophyll

EDTA:

Ethylene diamine tetra acidic acid

MBP:

Maltose-binding protein

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Acknowledgements

The author of this paper is thankful to the Research Institute for Fundamental Sciences (RIFS), University of Tabriz for the financial support.

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  1. Department of Biochemistry, University of Tabriz, Tabriz, Iran

    Ashraf Gholizadeh

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  1. Ashraf Gholizadeh
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Correspondence to Ashraf Gholizadeh.

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There is no conflict of interest in this study.

Appendix

Appendix

figure a

Prediction and comparison of primary, secondary and tertiary structures of WSCP1 and DUF538 proteins

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Gholizadeh, A. Chlorophyll Binding Ability of Non-chloroplastic DUF538 Protein Superfamily in Plants. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 967–976 (2018). https://doi.org/10.1007/s40011-016-0834-8

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