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Phosphorus Solubilization and Plant Growth Promotion Ability of Rhizobacteria of R. communis L Growing in Assam, India

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Abstract

Castor (Ricinus communis L) is the primary host plant of eri silkworm and its rhizosphere harbours diverse group of microbial community with biofertilizer potentiality. Phosphate solubilizing bacteria (PSB) render available phosphate (P) in agricultural soil by P mineralization process through enzyme mediated reaction. In search for PSB strains, 15 castor rhizobacteria were isolated and characterized for morphological and biochemical properties. The isolates were screened in vitro for P solubilization efficiency both qualitatively and quantitatively. Isolate MAJ PSB12 produced highest soluble P concentration (322.20 µmol/l) in National Botanical Research Institute Phosphate medium after 96 h of incubation with a maximum drop in pH to 5.4 from 7.0. Among the isolates, maximum content of IAA (24.6 mg/l) and GA3 (3.921 mg/l) was also found to be produced by the same strain. The most potential isolate was identified as Bacillus firmus MAJ PSB12 by 16S rRNA gene homology analysis and the sequence was submitted to National Centre for Biotechnology Information GenBank. Although many species belonging to the genus Bacillus are efficient P solubilizer, application of native rhizobacteria is easier for adaptation and succession during biofertilization process. B. firmus MAJ PSB12 can be utilized as potential biofertilizer to promote sustainable castor cultivation in sericulture for upliftment of rural livelihood.

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References

  1. Woo SM, Lee MK, Hong IS, Poonguzhali S, Sa TM (2010) Isolation and characterization of phosphate solubilizing bacteria from Chinese cabbage. In: 19th world congress of soil science, vol. 1, pp 56–59

  2. McLaughlin MJ, Tiller KG, Naidu R, Stevens DP (1996) Review: the behaviour and environmental impact of contaminants in fertilizers. Aus J Soil Res 34:1–54

    Article  CAS  Google Scholar 

  3. Bolan NS, Adriano DC, Naidu R (2003) Role of phosphorus in immobilization and bioavailability of heavy metals in the soil-plant system. Rev Environ Contam Toxicol 177:1–44

    PubMed  CAS  Google Scholar 

  4. He ZL, Zhang MK, Calvert DV, Stoffella PJ, Li YC (2003) Loading of phosphorus in surface runoff in relation to management practices and soil properties. Soil Crop Sci Soc Fla Proc 62:12–20

    Google Scholar 

  5. Sharpley AN, Daniel T, Sims T, Lemunyon J, Stevens R, Parry R (2003) Agricultural phosphorous and eutrophication, 2nd edn. Department of Agriculture, Agricultural Research Service, Lincoln, p 149

    Google Scholar 

  6. De Souza MJBD, Nair S, Chandramohan D (2000) Phosphate solubilizing bacteria around Indian Peninsula. Ind J Mar Sci 29:48–51

    Google Scholar 

  7. Afzal A, Ashraf M, Asad AS, Farooq M (2005) Effect of phosphate solubilizing microorganisms on phosphorus uptake, yield, and yield traits of wheat (Tritium aestivum) in rain fed area. Int J Agric Biol 7(2):207–209

    Google Scholar 

  8. Afzal A, Ashgari B (2008) Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticum aestivum). Int J Agric Biol 10(1):85–88

    CAS  Google Scholar 

  9. Ranjan A, Mahalakshmi MR, Sridevi M (2013) Isolation and characterization of phosphate-solubilizing bacterial species from different crop fields of Salem, Tamil Nadu, India. Int J Nutr Pharmacol Neurol Dis 3(1):29–33

    Article  CAS  Google Scholar 

  10. Widawati S (2011) Diversity and phosphate solubilization by bacteria isolated from laki island coastal ecosystem. Biodiversity 12(1):17–21

    Article  Google Scholar 

  11. Reyes I, Bernier L, Simard R, Antoun H (1999) Effect of nitrogen source on solubilization of different organic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants. FEMS Microbiol Ecol 28:281–290

    Article  CAS  Google Scholar 

  12. Kumar A, Kumar A, Devi S, Patil S, Chandani P, Nagi S (2012) Isolation, screening and characterization of bacteria from rhizospheric soils from different plant growth promotion activities: as in vitro study. Recent Res Sci Technol 4(1):1–5

    Google Scholar 

  13. Jones DL (1998) Organic acids in the rhizosphere—a critical review. Plant Soil 205(1):25–44

    Article  CAS  Google Scholar 

  14. Archana G, Buch A, Naresh KG (2012) Pivotal role of organic acid secretion by rhizobacteria in plant growth promotion. In: Satyanarayana T, Johri BN, Prakash A (eds) Microorganisms in sustainable agriculture and biotechnology. Springer, Berlin, p 3553

    Google Scholar 

  15. Fankem H, Nwaga D, Deubel A, Merbach W, Etoa FX (2006) Occurence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon. Afr J Biotechnol 5(24):2450–2460

    CAS  Google Scholar 

  16. Patel D, Parmar P (2013) Isolation and screening of phosphate solubilizing bacteria from sunflower rhizosphere. Glob J Biosci Biotechnol 2(3):438–441

    Google Scholar 

  17. Smibert RM, Kreig NR (1981) Generation characterization. In: Gerhard PM, Castillow RN, Nester EW, Wood WA, Kreig NR, Philips GB (eds) Manual of methods for general bacteriology. American Society for Microbiology, Rahway, pp 409–443

    Google Scholar 

  18. Holt JG, Krieg NR, Sneath PHA, Staley JT (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore, p 175

    Google Scholar 

  19. Edi Premono M, Moawad AM, Vlek PLG (1996) Effect of phosphate- solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indones J Crop Sci 11(1996):13–23

    Google Scholar 

  20. Islama T, Deoraa A, Hashidokoa Y, Rahmana A, Itoa T, Taharaa S (2007) Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh. Z Naturforsch 62(c):103–110

    Article  Google Scholar 

  21. Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chem Acta 27:31–36

    Article  CAS  Google Scholar 

  22. Wohler I (1997) Auxin-indole derivatives in soils determined by a colorimetric method and by high performance liquid chromatography. Microbiol Res 152:399–405

    Article  Google Scholar 

  23. Marques APGC, Pires C, Moreira H, Rangel AOSS, Castro PML (2010) Assessment of the plant growth promotion abilities using Zea mays as indicator plant. Soil Biol Biochem 42:1229–1235

    Article  CAS  Google Scholar 

  24. Paleg LG (1965) Physiological effects of gibberellins. Annu Rev Plant Physiol 16:291–322

    Article  CAS  Google Scholar 

  25. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208

    Article  CAS  Google Scholar 

  26. Weisberg WG, Barns SM, Pelletier BA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703

    Article  Google Scholar 

  27. Saitou N, Nie M (1987) The Neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  28. Chakraborty A, Hema MR, Rajgopal R, Jain M, Yadav R, Siddalingeshwara KG, Pramod T (2014) Isolation and characterization of potential plant growth promoting rhizobacteria from non-rhizospheric soil. Int J Curr Microbiol App Sci 3(4):432–438

    Google Scholar 

  29. Gulati A, Vyas P, Rahi P, Kasana RC (2009) Plant growth-promoting and rhizosphere-competent Acinetobacter rhizosphaerae strain BIHB 723 from the cold deserts of the Himalayas. Curr Microbiol 58:371–377

    Article  PubMed  CAS  Google Scholar 

  30. Rodriguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339

    Article  PubMed  CAS  Google Scholar 

  31. Raghu K, MacRae IC (1966) Occurrence of phosphate-dissolving microorganisms in the rhizosphere of rice plants and in submerged soils. J Appl Bacteriol 29:582–586

    Article  PubMed  CAS  Google Scholar 

  32. Coats VC, Rumpho ME (2014) The rhizosphere microbiota of plant invaders: an overview of recent advances in the microbiomics of invasive plants. Front Microbiol 5:368

    Article  PubMed  PubMed Central  Google Scholar 

  33. Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC (2006) Phosphate solubilizing bacteria from subtropical soil and their tri calcium phosphate solubilizing abilities. Appl Soil Ecol 34:33–41

    Article  Google Scholar 

  34. Hwangbo H, Park RD, Kim YW, Rim YS, Park KH, Kim TH, Suh JS, Kim KY (2003) 2-Ketogluconic acid production and phosphate solubilization by enterobacter intermedium. Curr Microbiol 47:87–92

    Article  PubMed  CAS  Google Scholar 

  35. Asea PEA, Kucey RMN, Stewart JWB (1988) Inorganic phosphate solubilization by two Penicillum species in solution culture and soil. Soil Biol Biochem 20:459–464

    Article  CAS  Google Scholar 

  36. Salih HM, Yahya AI, Rehman AA, Munam BH (1989) Availability of phosphorus in a calcareous soil treated with rock phosphate or super-phosphate or affected by phosphate dissolving fungi. Plant Soil 20:181–185

    Article  Google Scholar 

  37. Whitelaw MA, Harden TJ, Helyar KR (1999) Phosphate Solubilization in solution culture by the soil fungus Penicillum radicum. Soil Biol Biochem 32:655–665

    Article  Google Scholar 

  38. Illmer P, Schinner F (1992) Solubilization of inorganic calcium phosphate—solubilising mechanisms. Soil Biol Biochem 27:257–263

    Article  Google Scholar 

  39. Martens DA, Frankenberger WT WT Jr (1994) Assimilation of exogenous 2–14C indole acetic acid and 3–14C tryptophan exposed to the roots of three wheat varieties. Plant Soil 166:281–290

    Article  CAS  Google Scholar 

  40. Costacurta A, Vanderleyden J (1995) Synthesis of phytohormones by plant-associated bacteria. Crit Rev Microbiol 21:1–18

    Article  PubMed  Google Scholar 

  41. Tsavkelova EA, Cherdyntseva TA, Botina SG, Netrusov AI (2007) Bacteria associated with orchid roots and microbial production of auxin. Microbiol Res 162:69–76

    Article  PubMed  CAS  Google Scholar 

  42. Leinhos V, Vacek O (1994) Biosynthesis of auxins by phosphate solubilizing rhizobacteria from wheat and rye. Microbiol Res 149:31–35

    Article  CAS  Google Scholar 

  43. Thimann KV (1972) Physiological actions of auxins. In: Steward FC (ed) Plant physiology. Academic Press, New York, pp 63–123

    Google Scholar 

  44. Magray MSUD, Kumar A, Rawat AK, Srivastava S (2011) Identification of Escherichia coli through analysis of 16S rRNA and 16S-23S rRNA internal transcribed spacer region sequences. Bioinformation 6(10):370–371

    Article  PubMed  PubMed Central  Google Scholar 

  45. Banik S, Dey BK (1983) Phosphate solubilizing potentiality of the microorganisms capable of utilizing aluminium phosphate a sole phosphate source. Zentralbl Mikrobiol 138:17–23

    PubMed  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to the Director, Central Muga Eri Research and Training Institute, Central Silk Board, Lahdoigarh, Jorhat, Assam (India) for providing the facilities and Department of Science and Technology (DST), Govt. of India, New Delhi (India) for providing the fund to conduct the work.

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Authors and Affiliations

  1. Biotechnology Section, CMER&TI, Central Silk Board, Lahdoigarh, Jorhat, Assam, India

    Sosanka Protim Sandilya, Pinky Moni Bhuyan & Dip Kumar Gogoi

  2. Department of Life Sciences, Dibrugarh University, Dibrugarh, Assam, India

    Devid Kardong

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  1. Sosanka Protim Sandilya
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  3. Dip Kumar Gogoi
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Correspondence to Dip Kumar Gogoi.

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Sandilya, S.P., Bhuyan, P.M., Gogoi, D.K. et al. Phosphorus Solubilization and Plant Growth Promotion Ability of Rhizobacteria of R. communis L Growing in Assam, India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 959–966 (2018). https://doi.org/10.1007/s40011-016-0833-9

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