Circadian Rhythm of Nitrate Reductase Activity in Jatropha curcas Under Different Photoregimens

Abstract

Our knowledge on the circadian phenomenon in Jatropha curcas is limited. Therefore, we studied the activity of nitrate reductase (NR) in leaves of J. curcas under light and dark cycles (LD 12:12), continuous light (LL) and constant darkness (DD). We observed a statistically significant circadian rhythm in NR activity in LD. Unlike in DD, the circadian oscillation in NR activity persisted in continuous light, irrespective of different light intensities (LL 2 lx and 15 lx). The peak activity of NR appeared in the morning in LL 15 lx and forenoon in LD, while it reached a peak at night in LL 2 lx. This shows that rhythm in NR activity in J. curcas could be endogenous and that the light intensity might modulate acrophase of NR activity. Jatropha curcas could be considered as an ideal higher plant model for teaching NR activity rhythm to undergraduate and postgraduate students of biology streams.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. 1.

    Bose JC (1937) Plant autographs and their revelations. Macmillan Company, New York

    Google Scholar 

  2. 2.

    Hayama R, Coupland G (2003) Shedding light on the circadian clock and the photoperiodic control of flowering. Curr Opin Plant Biol 6:13–19

    CAS  Article  Google Scholar 

  3. 3.

    Mačukanović M, Blaženčić Ž (1998) Diurnal and seasonal dynamics of nectar secretion of some species in the family Lamiaceae. Acta Vet 48:173–183

    Google Scholar 

  4. 4.

    Tucker DE, Allen DJ, Ort DR (2004) Control of nitrate reductase by circadian and diurnal rhythms in tomato. Planta 219:277–285

    CAS  Article  Google Scholar 

  5. 5.

    Solomonson LP, Barber MJ (1990) Assimilatory nitrate reductase: functional properties and regulation. Annu Rev Plant Physiol Plant Mol Biol 41:225–253

    CAS  Article  Google Scholar 

  6. 6.

    Lillo C (1994) Light regulation of nitrate reductase in green leaves of higher plants. Physiol Plant 90:616–620

    CAS  Article  Google Scholar 

  7. 7.

    Lillo C, Meyer C, Ruoff P (2001) The nitrate reductase circadian system. The central clock dogma contra multiple oscillatory feedback loops. Plant Physiol 125:1554–1557

    CAS  Article  Google Scholar 

  8. 8.

    Huber JL, Huber SC, Campbell WH, Redinbaugh MG (1992) Reversible light/dark modulation of spinach leaf nitrate reductase activity involves protein phosphorylation. Arch Biochem Biophys 296:58–65

    CAS  Article  Google Scholar 

  9. 9.

    Lillo C (1984) Circadian rhythmicity of nitrate reductase activity in barley leaves. Physiol Plant 61:219–223

    CAS  Article  Google Scholar 

  10. 10.

    Chandrashekaran MK, Johnsson A, Engelmann W (1973) Possible ‘dawn’ and ‘dusk’ roles of light pulses shifting the phase of a circadian rhythm. J Comp Physiol 82:347–356

    Article  Google Scholar 

  11. 11.

    Deng W, Clausen J, Boden S, Oliver SN, Casao MC, Ford B, Anderssen RS, Trevaskis B (2015) Dawn and dusk set states of the circadian oscillator in sprouting barley (Hordeum vulgare) seedlings. PLoS ONE 10(6):e0129781

    Article  Google Scholar 

  12. 12.

    Ramaswamy O, Saxena IM, Mukherjee SG, Sopory SK (1983) Phytochrome regulation of nitrate reductase in wheat. J Biosci 5:63–69

    CAS  Article  Google Scholar 

  13. 13.

    Nelson W, Tong YL, Lee JK, Halberg F (1979) Methods of cosinor-rhythmometry. Chronobiologia 6:305–323

    CAS  PubMed  Google Scholar 

  14. 14.

    Deng MD, Moureaux T, Leydecker MT, Caboche M (1990) Nitrate-reductase expression is under the control of a circadian rhythm and is light inducible in Nicotiana tabacum leaves. Planta 180:257–261

    CAS  Article  Google Scholar 

  15. 15.

    Deng M, Moureaux T, Caboche M (1989) Tungstate, a molybdate analog inactivating nitrate reductase, deregulates the expression of the nitrate reductase structural gene. Plant Physiol 91:304–309

    CAS  Article  Google Scholar 

  16. 16.

    Galangau F, Daniel-Vedele F, Moreaux T, Dorbe MF, Leydecker MT, Caboche M (1988) Expression of leaf nitrate reductase gene from tomato and tobacco in relation to light–dark regimes and nitrate supply. Plant Physiol 88:383–388

    CAS  Article  Google Scholar 

  17. 17.

    Tucker DE, Ort DR (2002) Low temperature induces expression of nitrate reductase in tomato that temporarily overrides circadian regulation of activity. Photosynth Res 72:285–293

    CAS  Article  Google Scholar 

  18. 18.

    Millar AJ (2004) Input signals to the plant circadian clock. J Exp Bot 55:277–283

    CAS  Article  Google Scholar 

  19. 19.

    Fu W, Li P, Wu Y, Tang J (2012) Effects of different light intensities on anti-oxidative enzyme activity, quality and biomass in lettuce. Hortic Sci 39:129–134

    CAS  Article  Google Scholar 

  20. 20.

    Gordon AJ, Ryle GJA, Powell CE, Mitchell D (1980) Export, mobilization, and respiration of assimilates in uniculum barley during light and darkness. J Exp Bot 31:461–473

    Article  Google Scholar 

Download references

Acknowledgements

Financial assistance received from the Chhattisgarh Council of Science and Technology (CCOST) (Grant No. 311/CGCOST/08), Raipur, to carry out the research work, is gratefully acknowledged. We also thank Dr. P.K. Patra, Professor and Head Department of Biochemistry, Pt. J.N.M. Medical College, Raipur, Chhattisgarh, for providing guidance and support.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Suneeta Patra.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Significance statement The circadian rhythm in activity of nitrate reductase (NR) enzyme, essential for assimilation of nitrogen, in leaf of Jatropha curcas could be utilized as an ideal model to teach light-controlled NR activity rhythm in plants to students of biology. The endogenous circadian phase could be manipulated to time the nitrogen supplementation.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Patra, S., Mishra, Y., Pande, B. et al. Circadian Rhythm of Nitrate Reductase Activity in Jatropha curcas Under Different Photoregimens. Natl. Acad. Sci. Lett. 43, 195–199 (2020). https://doi.org/10.1007/s40009-019-00828-8

Download citation

Keywords

  • Jatropha curcas
  • Nitrate reductase
  • Circadian rhythm
  • LD 12:12
  • Continuous light (LL)
  • Constant darkness (DD)