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In Vitro Multiple Shoot Induction and Plant Regeneration from Male Ephedra foliata: A Potential Medicinal Gymnosperm

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Abstract

Ephedra foliata Boiss. Ex. C.A. Mey, known as soma plant according to ancient Indian Ayurvedic system. It is known for its potential to cure many diseases. Review of literature revealed that E. foliata contains traces of alkaloids responsible for its therapeutic activity. In present study effect of different concentrations of plant growth regulator on nodal sector explants was checked for multiple shoot production. Maximum numbers of shoots were observed on Kinetin (2.5 mg/l) whereas, Kinetin (2.5 mg/l) with Indole acetic acid (2.5 mg/l) induced 9.04 ± 0.8 shoots in 22.46 ± 0.57 days and percentage of explants that showed response was more than any other combination. Out of the two plant growth regulators used for root induction from in vitro grown shoots 1-napthaleneacetic acid showed better response than indole-3-acetic acid.

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References

  1. Price RA (1996) Systematics of the Gnetales: a review of morphological and molecular evidence. Int J Plant Sci 157:S40–S49

    Article  ADS  Google Scholar 

  2. Dhiman, M, Sharma V (2010) Regeneration of plants from somatic embryos derived from stem culture in Ephedra foliata. In: Role of Biotechnology in Food Security and Climatic Change. Proceeding of sixth international plant tissue culture and biotechnology conference, 2010. Bangladesh Assoc. Plant Tissue Cult. & Biotech. Dhaka, Bangladesh, pp 19–25

  3. Leung AY (1999) Ephedrine, Ephedra, Mahuang, Mahuanggen-what are they? Presented at AHPA Ephedra international symposium, Arlington, VA

  4. Leung AY, Foster S (1996) Encyclopedia of common natural ingredients. Wiley, New York

    Google Scholar 

  5. Caveney S, Charlet DA, Freitag H, Maier-Stolte M, Starratt AN (2001) New observations on the secondary chemistry of world Ephedra (Ephedraceae). Am J Bot 88:1199–1208

    Article  CAS  PubMed  Google Scholar 

  6. Tanaka T, Ohba K, Kawahara K, Sakei E (1995) Comparison of the constituents of Ephedra herbs from various countries on ephedrine type alkaloids. Nat Med 49:418–424

    CAS  Google Scholar 

  7. Kondo N, Mikage M, Idaka K (1999) Medico-botanical studies of Ephedra plants from the Himalayan region, Part III. Causitive factors of variation of alkaloid content in herbal stems. Nat Med 53:194–200

    Google Scholar 

  8. Kasahara Y, Hayasaka H, Oba K, Hikino H (1986) Seasonal Dynamics of the accumulation of ephedrine alkaloids in Ephedra distachya herbs. Shoyakugazu 39:142–145

    Google Scholar 

  9. Evans DA, Sharp WR, Medina-Filho HP (1984) Somaclonal and gametovlonal variation. Am J Bot 71:759–774

    Article  Google Scholar 

  10. Razdan MK (1995) An introduction to plant tissue culture. Oxford and IBH publishing Co., Pvt. Ltd, New Delhi, pp 27–91

    Google Scholar 

  11. Gamborg OL, Philips GC (eds) (1998) Plant cell, tissue and organ culture fundamental methods. Narosa Publishing House, New Delhi, pp 87–95

    Google Scholar 

  12. Gruce-Sorensen G, Spencer ID (1993) Biosynthesis of the Ephedra alkaloids: evolution of the c6–c3 skeleton. J Am Chem Soc 115:2052–2054

    Article  Google Scholar 

  13. Sagara K, Oshima T, Misaki T (1983) A simultaneous determination of norephedrine, pdeudoephedrine, ephedrine, and methylephedrine in Ephedra herba and oriental pharmaceutical preparations by ion-pair high-performance liquid chromatography. Chem Pharm Bull (Tokya) 31:2359–2365

    Article  CAS  Google Scholar 

  14. Qazilbach NA (1971) Pakistani Ephedra. Pharmacol Weekbl 106:345–349

    Google Scholar 

  15. Bhatnagar SP, Singh MN (1984) Organogenesis in the cultured female gametophytes of Ephedra foliata. J Exp Bot 35:268–278

    Article  Google Scholar 

  16. Gupta R, Sethi KL (1983) Conservation of medicinal plant resources in the Himalayan region. In: Jain SK, Mehra KL (eds) Conservation of tropical plant resources. In: Proceedings of workshop on conservation of tropical plant resources in South East Asia, New Delhi. Botanical Survey of India, Howrah, pp 101–109

  17. O’Dowd NA, McCauley PG, Richardson DHS, Wilson G (1993) Callus production, Suspension culture and in vitro alkaloid yields of Ephedra. Plant Cell Tissue Organ Cult 34:149–155

    Article  Google Scholar 

  18. O’Dowd NA, Richardson DHS (1993) In vitro micro-prorogation of Ephedra. J Hortic Sci 68:1013–1020

    Article  Google Scholar 

  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  20. Mousavi B, Saadat YA, Solouki M (2012) Factors affecting the shoot multiplication of Ephedra procera in iran. Trakia J Sci 10(1):16–21

    Google Scholar 

  21. Lodha D, Rathore N, Kataria V, Shekhawat NS (2014) In vitro propagation of female Ephedra foliata Boiss. & Kotschy ex Boiss.: an endemic and threatened Gymnosperm of the Thar Desert. Physiol Mol Biol Plant 20:375–383

    Article  CAS  Google Scholar 

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Acknowledgements

First author thankful to his research guide as well as authorities of Savitribai Phule Pune University for providing necessary facilities for carrying out present study.

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  1. Department of Botany, Savitribai Phule Pune University, Pune, 411007, India

    Akshay Joshi & Subhash Deokule

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  1. Akshay Joshi
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  2. Subhash Deokule
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Correspondence to Akshay Joshi.

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Joshi, A., Deokule, S. In Vitro Multiple Shoot Induction and Plant Regeneration from Male Ephedra foliata: A Potential Medicinal Gymnosperm. Natl. Acad. Sci. Lett. 42, 271–276 (2019). https://doi.org/10.1007/s40009-018-0714-2

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  • DOI: https://doi.org/10.1007/s40009-018-0714-2

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