In vitro and in vivo evaluation of a novel nitric oxide-releasing ointment for the treatment of methicillin-resistant Staphylococcus aureus-infected wounds



Nitric oxide (NO) has emerged as a novel agent for the treatment of infected wounds owing to its potent wound-healing effects and antibacterial activity against drug-resistant bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). In this study, we developed a NO-releasing ointment composed of S-nitrosoglutathione (GSNO) and polyethylene glycol (PEG) for the treatment of MRSA-infected cutaneous wounds.


The GSNO-incorporated PEG ointment (GPO) was successfully prepared by homogeneous dispersion of micronized GSNO in a PEG ointment base. High encapsulation efficiency was achieved (97.25%) via water-free fabrication processing of the GPO, resulting in minimal GSNO hydrolysis.


When applied to a wound, the wound fluid triggered the degradation of GSNO and NO was released from the GPO for 24 h without an initial burst release. The GPO exhibited potent antibacterial effects against MRSA without cytotoxic effects against L929 cells. An in vivo wound healing experiment using a mouse MRSA-challenged full-thickness wound model revealed that the GPO could facilitate healing of infected wounds.


Thus, the GPO could be a promising NO-releasing formulation for the treatment of infected cutaneous wounds.

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This work was supported by a 2-year research grant from Pusan National University.

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Correspondence to Jin-Wook Yoo.

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All animal experiments were reviewed and approved by the Pusan National University Institutional Animal Care and Use Committee (PNU-IACUC) on 01 February 2018 (PNU-2018-1800).

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Lee, J., Hlaing, S.P., Cao, J. et al. In vitro and in vivo evaluation of a novel nitric oxide-releasing ointment for the treatment of methicillin-resistant Staphylococcus aureus-infected wounds. J. Pharm. Investig. 50, 505–512 (2020).

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  • Nitric oxide
  • S-nitrosoglutathione
  • Methicillin-resistant staphylococcus aureus
  • Infected wound healing
  • Ointment