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Micromorphological and Anatomical Evaluation of In Vitro and Field Transferred Plants of Coccinia indica

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Abstract

The micromorphological and anatomical changes in micropropagated plantlets of Coccinia indica grown under field conditions were studied. In vitro regeneration of plantlets under heterotrophic conditions is responsible for the induction of plant’s physiological and structural modifications. Significant developmental changes were observed from in vitro to the in vivo shifted plantlets. The micromorphological alterations in terms of stomatal index (from 23.0 to 19.2), vein islets (from 6.2 to 11.5), trichomes (from 11.0 to 16.0), epidermal cells and mesophyll tissues revealed the effective metabolism of photosynthesis and respiration from in vitro to field environment. Abnormal hypertrophied cortical cells with large intercellular spaces and retarded vascular cells were reported from the in vitro grown stem and root, whereas compact storage cells and advanced vascular tissues with the development of secondary xylem and phloem tissues were observed in field transferred plants. Field transfer is stressful to the micropropagated plantlets, which could be repaired by maintaining in vitro regenerated plantlets in the greenhouse to induce the plasticity in the plantlets. This study could help in understanding the abnormal tissues organization and functions of stomata behind the poor acclimatization process which leads to the poor survival of micropropagated plantlets in the field.

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Abbreviations

BAP:

6-benzylaminopurine

FAA:

Formaldehyde acetic acid ethyl alcohol

IBA:

Indole-3 butyric acid

Kin:

Kinetin

MS:

Murashige and Skoog’s medium

References

  1. Ali MA, Al-Hemaid FMA (2011) Taxonomic significance of trichomes micromorphology in cucurbits. Saudi J Biol Sci 18:87–92

    Article  PubMed  Google Scholar 

  2. Batagin-Piotto KD, De Almeida CV, Piotto FA, De Almeida M (2012) Anatomical analysis of peach palm (Bactris gasipaes) leaves cultivated in vitro, ex vitro and in vivo. Braz J Bot 35:71–78

    Article  Google Scholar 

  3. Batkova P, Pospisilova J, Synkova H (2008) Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. Biol Plant 52:413–422

    Article  CAS  Google Scholar 

  4. Chirinea CF, Pasqual M, De Araujo AG, Pereira AR, De Castro EM (2012) Acclimatization and leaf anatomy of micropropagated fig plantlets. Rev Bras Frutic 34:1180–1188

    Article  Google Scholar 

  5. Cooke CIET (1903) The flora of the presidency of Bombay. The Horticulture Society, New York

    Book  Google Scholar 

  6. Costa FHS, Pasqual M, Pereira JES, de Castro EM (2009) Anatomical and physiological modifications of micropropagated ‘Caipira’ Banana plants under natural light. Sci Agric (Piracicaba, Braz) 66:323–330

    Article  Google Scholar 

  7. Deokate UA, Khadabadi SS (2012) Pharmacology and phytochemistry of Coccinia indica. Pharmacophore 3:179–185

    CAS  Google Scholar 

  8. El-Zaher MHA (2008) Studies on micropropagation of Jackfruit 1- behavior of the jackfruit plants through the micropropagation stages. World J Agric Sci 4:263–279

    Google Scholar 

  9. Facciola S (1990) Cornucopia: a source book of edible plants. Kampong Publications, Vista

    Google Scholar 

  10. Gradist P, Purintrapiban J (2009) The biological activity of Coccinia indica on glucose transporter 1(GLUT1) promoter. Songklanakarni J Sci Technol 31:247–253

    Google Scholar 

  11. Hazarika BN (2003) Acclimatization of tissue-cultured plants. Curr Sci 85:1704–1712

    CAS  Google Scholar 

  12. Hickey LJ, Wolfe JA (1975) The bases of angiosperm phylogeny, vegetative morphology. Ann Missouri Bot Gard 62:538–589

    Article  Google Scholar 

  13. Jain SM (2001) Tissue culture derived variation in crop improvement. Euphytica 118:153–166

    Article  CAS  Google Scholar 

  14. Johansen DA (1940) Plant microtechnique. McGraw Hill Book Co., New York, pp 182–197

    Google Scholar 

  15. Kubota C (2002) Photoautotrophic micropropagation: importance of controlled environment in plant tissue culture. Comb Proc Int Plant Propag Soc 52:609–613

    Google Scholar 

  16. Lodha D, Patel AK, Shekhawat NS (2015) A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub. Physiol Mol Biol Plants 21:407–415

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  17. Luis ZG, Bezerra KMG, Scherwinski-Pereira JE (2010) Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds. Braz J Plant Physiol 22:209–215

    Article  Google Scholar 

  18. Mathur S, Shekhawat GS, Batra A (2008) Somatic embryogenesis from cotyledon explants of Salvodora persica L. Phytomorphol 58:57–63

    Google Scholar 

  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479

    Article  CAS  Google Scholar 

  20. Patel AR, Ishnava KB (2015) In vitro shoot multiplication from nodal explants of Coccinia grandis (L.) Voigt. and it’s antidiabetic and antioxidant activity. Asian J Biol Sci 8:57–71

    Article  CAS  Google Scholar 

  21. Paunescu A (2008) In vitro and in vivo variability of histological traits of Dianthus callizonus (Caryophyllaceae) aerial vegetative organs. Phytol Balc 144:17–423

    Google Scholar 

  22. Pospisilova J, Catsky J, Sestak Z (1997) Photosynthesis in plants cultivated in vitro. In: Pessarakli M (ed) Handbook of photosynthesis. Marcel Dekker Inc, New York

    Google Scholar 

  23. Pospisilova J, Haisel D, Synkova H, Atsky J, Wilhelmova N, Plzakova S, Prochazkova D, Ramek F (2000) Photosynthetic pigments and gas exchange during ex vitro acclimatization of tobacco plantlets as affected by CO2 supply and abscisic acid. Plant Cell Tissue Org Cult 61:125–133

    Article  CAS  Google Scholar 

  24. Pospisilova J, Ticha I, Kadleek P, Haisel D, Plzakova S (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42:481–497

    Article  Google Scholar 

  25. Prabhakar M (2004) Structure, delimitation, nomenclature and classification of stomata. Acta Bot Sin 46:242–252

    Google Scholar 

  26. Preece JE, Sutter EG (1991) Acclimatization of micropropagation plants to the greenhouse and field. In: Debergh PC, Zimmerman RD (eds) Micropropagation technology and application. Kluwer Academic Publishers, Dordrecht, pp 77–93

    Google Scholar 

  27. Rathore NS, Rathore N, Shekhawat NS (2013) In vitro propagation and micromorphological studies of Cleome gynandra: a C4 model plant closely related to Arabidopsis thaliana. Acta Physiol Plant 35:2691–2698

    Article  Google Scholar 

  28. Salisbury EJ (1927) On the causes and ecological significance of stomatal frequency, with special reference to the woodland flora. Philos Trans R Soc Lond B 216:1–65

    Article  Google Scholar 

  29. Sarker PFMS, Jahan R, Rahmatullah M (2009) In vitro regeneration of Coccinia grandis (L.) Voigt. an indigenous medicinal plant of Bangladesh. Afr J Tradit Complement Altern Med (Abst). World Congress on Medicinal and Aromatic Plants, Cape Town, South Africa

  30. Sass JE (1940) Elements of botanical microtechnique. McFraw-Hill Book Co, New York, p 222

    Google Scholar 

  31. Shekhawat MS, Manokari M (2016) In vitro propagation, micromorphological studies and ex vitro rooting of cannon ball tree (Couroupita guianensis aubl.): a multipurpose threatened species. Physiol Mol Biol Plants 22:131–142

    Article  PubMed  PubMed Central  Google Scholar 

  32. Shekhawat MS, Manokari M (2016) In vitro regeneration frequency, micro-morphological studies and ex vitro rooting of Hemidesmus indicus (L.) R. Br.: multi-potent endangered climber. Ind J Plant Physiol 21:151–160

    Article  CAS  Google Scholar 

  33. Shekhawat MS, Ravindran CP, Manokari M (2014) Development of hormonal regulation of direct shoots and roots regeneration in Coccinia indica L. Int J Nat Sci Res 2:103–112

    Google Scholar 

  34. Silva LM, Alquini Y, Cavallet VJ (2005) Inter-relações entre a anatomia vegetal e a a produção vegetal. Acta Bot Bras 19:183–194

    Article  Google Scholar 

  35. Souza TC, Magalhaes PC, Pereira FJ, Castro EM, Silva JM, Parentoni SN (2010) Leaf plasticity in successive selection cycles of ‘Saracura’ maize in response to periodic soil flooding. Pesqui Agropecu Bras 45:16–24

    Article  Google Scholar 

  36. Taiz L, Zeiger E (2006) Plant physiology. Sinauer Associates Inc, Sunderland

    Google Scholar 

  37. Taware AS, Mukadam DS, Chavan AM, Taware SD (2010) Comparative studies of in vitro and in vivo grown plants and callus of Stevia rebaudiana (Bertoni). Int J Integr Biol 9:10–15

    Google Scholar 

  38. Thiripurasundari U, Rao MV (2012) Indirect organogenesis from nodal explants of Coccinia grandis (L.) Voigt. Indian J Biotechnol 11:352–354

    CAS  Google Scholar 

  39. Wetmore R, Wardlaw CW (1951) Experimental morphogenesis in vascular plants. Ann Rev Plant Physiol 2:269–292

    Article  CAS  Google Scholar 

  40. Yadav G, Mishra A (2010) Medical properties of Ivy gourd (Cephalandra indica): a review. Int J Pharma Res Dev 2:92–98

    Google Scholar 

Download references

Acknowledgement

Authors are grateful to the Department of Science, Technology and Environment, Government of Puducherry, India, for providing financial support to their laboratory as Grant–In-Aid Scheme.

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Authors and Affiliations

  1. Biotechnology Laboratory, Department of Plant Science, M.G.G.A.C, Mahe, Pondicherry, India

    Mahipal S. Shekhawat

  2. Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry, India

    M. Manokari

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  1. Mahipal S. Shekhawat
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  2. M. Manokari
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Contributions

MM designed and performed the experiments. MSS interpreted the results and wrote the manuscript.

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Correspondence to Mahipal S. Shekhawat.

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Shekhawat, M.S., Manokari, M. Micromorphological and Anatomical Evaluation of In Vitro and Field Transferred Plants of Coccinia indica. Agric Res 7, 135–144 (2018). https://doi.org/10.1007/s40003-018-0326-6

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  • DOI: https://doi.org/10.1007/s40003-018-0326-6

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