Agricultural Research

, Volume 7, Issue 2, pp 135–144 | Cite as

Micromorphological and Anatomical Evaluation of In Vitro and Field Transferred Plants of Coccinia indica

  • Mahipal S. ShekhawatEmail author
  • M. Manokari
Full-Length Research Article


The micromorphological and anatomical changes in micropropagated plantlets of Coccinia indica grown under field conditions were studied. In vitro regeneration of plantlets under heterotrophic conditions is responsible for the induction of plant’s physiological and structural modifications. Significant developmental changes were observed from in vitro to the in vivo shifted plantlets. The micromorphological alterations in terms of stomatal index (from 23.0 to 19.2), vein islets (from 6.2 to 11.5), trichomes (from 11.0 to 16.0), epidermal cells and mesophyll tissues revealed the effective metabolism of photosynthesis and respiration from in vitro to field environment. Abnormal hypertrophied cortical cells with large intercellular spaces and retarded vascular cells were reported from the in vitro grown stem and root, whereas compact storage cells and advanced vascular tissues with the development of secondary xylem and phloem tissues were observed in field transferred plants. Field transfer is stressful to the micropropagated plantlets, which could be repaired by maintaining in vitro regenerated plantlets in the greenhouse to induce the plasticity in the plantlets. This study could help in understanding the abnormal tissues organization and functions of stomata behind the poor acclimatization process which leads to the poor survival of micropropagated plantlets in the field.


Acclimatization Coccinia indica Anatomy Morphology Micropropagation 





Formaldehyde acetic acid ethyl alcohol


Indole-3 butyric acid




Murashige and Skoog’s medium



Authors are grateful to the Department of Science, Technology and Environment, Government of Puducherry, India, for providing financial support to their laboratory as Grant–In-Aid Scheme.

Authors’ Contribution

MM designed and performed the experiments. MSS interpreted the results and wrote the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest in this publication.


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Copyright information

© NAAS (National Academy of Agricultural Sciences) 2018

Authors and Affiliations

  1. 1.Biotechnology Laboratory, Department of Plant ScienceM.G.G.A.CMahe, PondicherryIndia
  2. 2.Department of BotanyK.M. Centre for Post Graduate StudiesPondicherryIndia

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