Micromorphological and Anatomical Evaluation of In Vitro and Field Transferred Plants of Coccinia indica
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The micromorphological and anatomical changes in micropropagated plantlets of Coccinia indica grown under field conditions were studied. In vitro regeneration of plantlets under heterotrophic conditions is responsible for the induction of plant’s physiological and structural modifications. Significant developmental changes were observed from in vitro to the in vivo shifted plantlets. The micromorphological alterations in terms of stomatal index (from 23.0 to 19.2), vein islets (from 6.2 to 11.5), trichomes (from 11.0 to 16.0), epidermal cells and mesophyll tissues revealed the effective metabolism of photosynthesis and respiration from in vitro to field environment. Abnormal hypertrophied cortical cells with large intercellular spaces and retarded vascular cells were reported from the in vitro grown stem and root, whereas compact storage cells and advanced vascular tissues with the development of secondary xylem and phloem tissues were observed in field transferred plants. Field transfer is stressful to the micropropagated plantlets, which could be repaired by maintaining in vitro regenerated plantlets in the greenhouse to induce the plasticity in the plantlets. This study could help in understanding the abnormal tissues organization and functions of stomata behind the poor acclimatization process which leads to the poor survival of micropropagated plantlets in the field.
KeywordsAcclimatization Coccinia indica Anatomy Morphology Micropropagation
Formaldehyde acetic acid ethyl alcohol
Indole-3 butyric acid
Murashige and Skoog’s medium
Authors are grateful to the Department of Science, Technology and Environment, Government of Puducherry, India, for providing financial support to their laboratory as Grant–In-Aid Scheme.
MM designed and performed the experiments. MSS interpreted the results and wrote the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest in this publication.
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