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Detection and quantification of EBV, HHV-6 and CMV DNA in the gastrointestinal tract of HIV-positive patients

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Abstract

Human herpes viruses (HHVs) have been frequently detected in the gastrointestinal (GI) tract and may contribute to the development of gastric cancer. In the present study, the detection rate and viral load of Epstein Barr virus (EBV), HHV-6 and Cytomegalovirus (CMV) were assessed in the GI tract of human immunodeficiency virus (HIV) positive patients and of uninfected patients. The analysis revealed a significantly higher detection rate of EBV and HHV-6 in HIV-infected individuals than in uninfected subjects (88.5 vs 63 %; p = 0.03). Moreover, EBV DNA load was significantly higher in the stomach of HIV patients than in controls. These data suggest that the HIV infection status may increase the persistence of these viruses in the GI compartment. Intriguingly, CMV DNA was undetectable in all biopsy specimens analyzed.

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Acknowledgements

The authors thank the Elitech group for providing EBV ELITe MGB®, CMV ELITe MGB® and HHV-6 ELITe MGB® kits.

Conflict of interest

On behalf of all authors the corresponding author states that there is no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Molecular Medicine, Sapienza University, Viale dell’Università 31, 00185, Rome, Italy

    F. Falasca, P. Maida, G. Antonelli & O. Turriziani

  2. Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy

    A. Gaeta & S. Verzaro

  3. Department of Clinical Medicine, Sapienza University, Rome, Italy

    I. Mezzaroma, A. Fantauzzi & G. Donato

  4. Department of Experimental Medicine, Sapienza University, Rome, Italy

    E. Bonci

  5. Laboratory of Virology, National Institute for Infectious Diseases Lazzaro Spallanzani, Rome, Italy

    C. Castilletti

Authors
  1. F. Falasca
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  2. P. Maida
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  3. A. Gaeta
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  4. S. Verzaro
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  5. I. Mezzaroma
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  6. A. Fantauzzi
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  7. G. Donato
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  8. E. Bonci
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  9. C. Castilletti
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  10. G. Antonelli
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  11. O. Turriziani
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Corresponding author

Correspondence to O. Turriziani.

Additional information

The authors F. Falasca, and P. Maida contributed equally to this study

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Falasca, F., Maida, P., Gaeta, A. et al. Detection and quantification of EBV, HHV-6 and CMV DNA in the gastrointestinal tract of HIV-positive patients. Infection 42, 1033–1037 (2014). https://doi.org/10.1007/s15010-014-0690-5

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  • DOI: https://doi.org/10.1007/s15010-014-0690-5

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