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Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

To investigate whether human adipose-derived stem cells (hADSCs) seeded on multilayered poly (l-lactide-co-É›-caprolactone) (PLCL) sheets improve bladder function in a rat model of detrusor smooth muscle-removed bladder.

Methods:

Male rats were randomly divided into 4 groups: Normal, injury (detrusor smooth muscle-removed bladder), PLCL (detrusor smooth muscle-removed bladder implanted with PLCL sheets), and PLCL + ADSC (detrusor smooth muscle-removed bladder implanted with PLCL sheets seeded with hADSCs). Four weeks after the treatment, physiological, histological, immunohistochemical, and immunoblot analyses were performed.

Results:

hADSCs were compatible with PLCL sheets. Further, the physiological study of PLCL + ADSC group showed significant improvement in compliance and contractility suggesting the functional improvement of the bladder. Histological, immunohistochemical and immunoblot analyses revealed the uniform distribution of hADSCs in between PLCL sheets as well as differentiation of hADSCs into smooth muscle cells (SMC) which is illustrated by the expression of SMC markers.

Conclusion:

hADSCs seeded on the multilayered PLCL sheets has the potential to differentiate into SMC, thus facilitating the recovery of compliance and contractility of the injured bladder.

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Acknowledgement

This research was supported by the National Research Foundation of Korea grant funded by the Korea government (MSIP) (No. 2011-0030075).

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Authors and Affiliations

  1. Department of Urology, College of Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea

    Kshitiz Raj Shrestha, Seung Hwan Jeon, Ae Ryang Jung, In Gul Kim, Ga Eun Kim, Yong Hyun Park & Ji Youl Lee

  2. Cancer Research Institute, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea

    Kshitiz Raj Shrestha, Ae Ryang Jung, In Gul Kim, Ga Eun Kim, Yong Hyun Park & Ji Youl Lee

  3. Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792, Republic of Korea

    Soo Hyun Kim

  4. Department of Bioinformatics, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, Republic of Korea

    Ji Youl Lee

Authors
  1. Kshitiz Raj Shrestha
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  2. Seung Hwan Jeon
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Correspondence to Ji Youl Lee.

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Ethical statement

The animal studies were performed after receiving approval of the Institutional Animal Care and Use Committee (IACUC) in The Catholic University of Korea, Seoul, Korea (IACUC Approval No. 2013-0111-03).

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Shrestha, K.R., Jeon, S.H., Jung, A.R. et al. Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function. Tissue Eng Regen Med 16, 201–212 (2019). https://doi.org/10.1007/s13770-019-00187-x

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  • DOI: https://doi.org/10.1007/s13770-019-00187-x

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