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Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Μonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films.

Methods:

For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiO2ntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells’ genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton’s Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay.

Results:

The highest differentiation capacity of MOMCs was observed on PCL/SiO2ntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation.

Conclusion:

To conclude, the differentiation of MOMCs was selectively guided by incorporating SiO2ntbs or SrHAnrds into a polymeric matrix, for the first time.

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Acknowledgements

The authors thank Biohellenika SA Biotechnology Company for providing the facilities and consumables that enabled the completion of this study. EG and GK conceived the study; MN and DB fabricated and characterized the materials; IK and EG designed and performed the experiments (equal contribution to this work); IK, EG, EP, MK and DB analysed the data; IK, EG and MN wrote the manuscript. All authors reviewed the manuscript.

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Author notes

  1. Iro Koliakou and Eleni Gounari authors have contributed equally to this work.

Authors and Affiliations

  1. Department of Biology, Laboratory of Animal Physiology, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    Iro Koliakou & Martha Kaloyianni

  2. Biohellenika Biotechnology Company, 65 Leoforos Georgikis Scholis, 57001, Thessaloníki, Greece

    Iro Koliakou, Eleni Gounari & George Koliakos

  3. Department of Biochemistry, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    Eleni Gounari & George Koliakos

  4. Department of Chemistry, Laboratory of Polymer Chemistry and Technology, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    Maria Nerantzaki & Dimitrios Bikiaris

  5. PHysico-Chimie des Electrolytes et Nanosystèmes InterfaciauX (PHENIX), Sorbonne Université, 75005, Paris, France

    Maria Nerantzaki

  6. Department of Physics, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece

    Eleni Pavlidou

Authors
  1. Iro Koliakou
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  2. Eleni Gounari
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Correspondence to Eleni Gounari or Maria Nerantzaki.

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Ethical statement

This study was approved by the Ethics Committee of Aristotle University of Thessaloniki, School of Medicine (390-9/1.7.2017) and University Hospital AXEPA of Thessaloniki (3868/24.1.2018). This research involves Human Participants after their informed consent. Peripheral blood samples were collected from healthy volunteer donors while mesenchymal stromal cells and HUVEC were isolated from Wharton’s jelly after parents approval during stem cell banking in Biohellenika SA Biotechnology Company.

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Koliakou, I., Gounari, E., Nerantzaki, M. et al. Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films. Tissue Eng Regen Med 16, 161–175 (2019). https://doi.org/10.1007/s13770-019-00185-z

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  • DOI: https://doi.org/10.1007/s13770-019-00185-z

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