Applied Biological Chemistry

, Volume 61, Issue 2, pp 251–255 | Cite as

Analysis of the biodistribution of natural products in mice by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

  • Hye-Jeong Hwang
  • Inseong Choi
  • Yoon Young Kang
  • Hyejung Mok
  • Yoongho Lim
  • Woon-Seok Yeo
Note
  • 61 Downloads

Abstract

Natural products originating from plants have various beneficial pharmacological effects, such as anticancer, antimicrobial, and anti-inflammatory activities, while being nontoxic. Therefore, tremendous efforts have been invested in understanding their bioactivities in the body to facilitate therapeutic target validation. However, such research is still challenging for certain natural products, such as flavonoids, which are rapidly metabolized in and eliminated from the human body. To investigate the bioactivities of such products, particularly in certain tissues, it is necessary to understand their biodistribution in vivo. In this respect, reliable analytical methods with simple and efficient procedures for the in vivo evaluation of natural small molecules are urgently required. In particular, mass spectrometry (MS) can be effectively used to analyze small molecules after tissue extraction, as MS has various advantages including accuracy, simplicity, and high sensitivity. Herein, we report the biodistribution of a natural small molecule by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). After intravenously injecting gomisin H into a mouse as a model natural product, it was extracted from each organ and then analyzed by MALDI-TOF MS. The analysis showed that gomisin H accumulated mainly in the liver and relatively large amounts of the product existed in the kidney and brain compared to those in other tissues.

Keywords

Biodistribution Gomisin Mass spectrometry Natural products Tissues 

Notes

Acknowledgments

This research was supported by the National Research Foundation (NRF) of Korea (NRF-2016R1D1A1A09918111) funded by the Ministry of Education and the Agri-Bio Industry Technology Development Program [316028-3, Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET)].

Supplementary material

13765_2018_353_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 23 kb)

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Copyright information

© The Korean Society for Applied Biological Chemistry 2018

Authors and Affiliations

  1. 1.Department of Bioscience and Biotechnology, Bio/Molecular Informatics CenterKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Bioscience and BiotechnologyKonkuk UniversitySeoulRepublic of Korea

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