Applied Biological Chemistry

, Volume 61, Issue 2, pp 227–233 | Cite as

Anti-inflammatory and immune-enhancing effects of enzyme-treated royal jelly

  • Hyejung Gu
  • In-Bong Song
  • Hye-Ju Han
  • Na-Young Lee
  • Ji-Yun Cha
  • Yeon-Kyong Son
  • Jungkee Kwon
Article
  • 41 Downloads

Abstract

Royal jelly is produced by honeybees and has been shown to be various pharmacologically active. Enzyme-treated royal jelly (ERJ) is an allergen-free form of royal jelly that has been converted to shorter easy-to-absorb chain monomers. In this study, we investigated the anti-inflammatory and immunomodulatory effects of ERJ on macrophages and mice. We found that ERJ altered macrophage proliferation and was protective against lipopolysaccharide (LPS)-induced stress. The mice, fed ERJ for 4 weeks and stimulated LPS, significantly reduced levels of tumor necrosis factor-alpha, interleukins-1, 6, 10, 12, and interferon gamma compared to control mice. ERJ significantly increased the proliferation of B-lymphocytes and T-lymphocytes, as well as the activity of natural killer cells in a dose-dependent manner. Therefore, our results indicate that ERJ has strong anti-inflammatory and immune-promoting activities and can be developed as a potential food material for prevention of inflammatory disease.

Keywords

Anti-inflammation Enzyme Immunity Royal jelly 

Notes

Acknowledgments

This work (C0453904) was supported by the Business for Cooperative R&D between Industry, Academy, and Research Institute funded by the Korea Small and Medium Business Administration in 2017.

References

  1. 1.
    Jeong JB, Hong SC, Jeong HJ, Koo JS (2012) Anti-inflammatory effects of ethyl acetate fraction from cnidium officinale Makino on LPS-stimulated RAW 264.7 and THP-1 cells. Korean J Plant Res 25:299–307CrossRefGoogle Scholar
  2. 2.
    Moller B, Villiger PM (2006) Inhibition of IL-1, IL-6, and TNF-alpha in immune-mediated inflammatory diseases. Springer Semin Immunopathol 27:391–408CrossRefGoogle Scholar
  3. 3.
    Lee JS, Hong EK (2011) Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris. Int Immunopharmacol 11:1226–1233CrossRefGoogle Scholar
  4. 4.
    Yoo SA, Kim OK, Nam DE, Kim Y, Baek H, Jun W, Lee J (2014) Immunomodulatory effects of fermented Curcuma longa L. extracts on RAW 264.7 cells. J Korean Soc Food Sci Nutr 43:216–223CrossRefGoogle Scholar
  5. 5.
    Guo H, Saiga A, Sato M, Miyazawa I, Shibata M, Takahata Y, Morimatsu F (2007) Royal jelly supplementation improves lipoprotein metabolism in human. J Nutr Sci Vitaminol 53:345–348CrossRefGoogle Scholar
  6. 6.
    Kohno K, Okamoto I, Sano O, Arai N, Iwaki K, Ikeda M, Kurimoto M (2004) Royal jelly inhibits the production of proinflammatory cytokines by activated macrophages. Biosci Biotechnol Biochem 68:138–145CrossRefGoogle Scholar
  7. 7.
    Seo MJ, Kang BW, Kim MJ, Lee HH, Seo KI, Kim KH, Jeong YK (2014) The effect of cordycepin on the production of pro-inflammatory cytokines in mouse peritoneal macrophages. Korean J Food Sci Technol 46:68–72CrossRefGoogle Scholar
  8. 8.
    Ryu HS, Kim J, Kim HS (2006) Enhancing effect of Sorghum bicolor L. Moench (Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean Soc Food Nutr 19:176–182Google Scholar
  9. 9.
    Kang IS, Kim RI, Kim GS, Kim NR, Shin JY, Kim C (2015) Effects of Agaricus blazei Murill water extract on immune response in BALB/c mice. J Korean Soc Food Sci Nutr 44:1629–1636CrossRefGoogle Scholar
  10. 10.
    Andrikopoulos S, Blair AR, Deluca N, Fam BC, Proietto J (2008) Evaluating the glucose tolerance test in mice. Am J Physiol Endocrinol Metab 295:1323–1332CrossRefGoogle Scholar
  11. 11.
    Townsend GF, Brown WH, Felauer EE, Townsend BH (1961) Studies on the in vitro antitumor activity of fatty acids: IV. The esters of acids closely related to 10-hydroxy-2-decenoic acid from royal jelly against transplantable mouse leukemia. Can J Biochem Physiol 39:1765–1770CrossRefGoogle Scholar
  12. 12.
    Birk RW, Gratchev A, Hakiy N, Politz O, Schledzewski K, Guillot P, Orfanos CE, Goerdt S (2001) Alternative activation of antigen-presenting cells: concepts and clinical relevance. Hautarzt 52:193–200CrossRefGoogle Scholar
  13. 13.
    Beutler B (2004) Innate immunity: an overview. Mol Immunol 40:845–859CrossRefGoogle Scholar
  14. 14.
    Mu MM, Chakravortty D, Sugiyama T, Koide N, Takahashi K, Mori I, Yoshida T, Yokochi T (2001) The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. J Endotoxin Res 7:431–438CrossRefGoogle Scholar
  15. 15.
    Park SH, Kim MJ, Kim GE, Park SY, Kim KBWR, Kim YJ, Cho YJ, Ahn DH (2017) Immuno-enhancing effect of enzymatic extract of Sargassum coreanum using crude enzyme from Shewanella oneidensis PKA 1008. J Korean Soc Food Sci Nutr 46:919–928Google Scholar
  16. 16.
    Dienz O, Rincon M (2009) The effects of IL-6 on CD4 T cell responses. Clin Immunol 130(1):27–33CrossRefGoogle Scholar
  17. 17.
    Fiorentino DF, Zlotnik A, Vieira P, Mosmann MTR, Howard KWM, O’Garra A (2017) IL-10 acts on the antigen-presenting cell to inhibit cytokine production by Th1 cells. J Immunol 146:3444–3451Google Scholar
  18. 18.
    Zalys R, Ian SZ, Robert HB, Lang CM, Patricia JM (2000) In vivo effects of chronic treatment with [Met5]-enkephalin on hematological values and natural killer cell activity in athymic mice. Life Sci 66:829–834CrossRefGoogle Scholar
  19. 19.
    Vivier E, Tomasello E, Baratin M, Walzer T, Ugolini S (2008) Functions of natural killer cells. Nat Immunol 9:503–510CrossRefGoogle Scholar

Copyright information

© The Korean Society for Applied Biological Chemistry 2018

Authors and Affiliations

  • Hyejung Gu
    • 1
  • In-Bong Song
    • 1
  • Hye-Ju Han
    • 1
  • Na-Young Lee
    • 2
  • Ji-Yun Cha
    • 2
  • Yeon-Kyong Son
    • 2
  • Jungkee Kwon
    • 1
  1. 1.Department of Laboratory Animal Medicine, College of Veterinary MedicineChonbuk National UniversityIksanRepublic of Korea
  2. 2.R&D Team, Food and Supplement Health ClaimsVitechJeonjuRepublic of Korea

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