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Applied Biological Chemistry

, Volume 61, Issue 1, pp 79–89 | Cite as

Application of electron beam irradiation for improving the microbial quality of processed laver products and luminescence detection of irradiated lavers

  • Eun-Jin Lee
  • Gui-Ran Kim
  • Kashif Ameer
  • Hyun-Kyu Kyung
  • Joong-Ho Kwon
Article
  • 90 Downloads

Abstract

The laver (Porphyra spp.) is normally processed in three kinds of products: dried laver (DL), roasted laver (RL), and seasoned roasted laver (SL). This work evaluated the effects of electron beam (E-beam) irradiation at different doses (0, 1, 4, 7, and 10 kGy) on microbiological and physicochemical qualities and detection characteristics of irradiated samples by luminescence analysis. E-beam irradiation resulted in dose-dependent microbial reductions, showing that 1 kGy destroyed initial coliforms (< 2.35 log CFU/g) to undetectable levels (< 10 CFU/g), while 7 kGy (approved dose for seaweed in Korea Food Code) reduced total aerobic bacteria (3.72–6.33 log CFU/g) and yeasts and molds (2.05–4.98 log CFU/g) by about 2 log cycles. Chlorophyll content remained unaffected in irradiated samples as compared to control; however, carotenoids content and Hunter’s b values (degree of yellowness) showed a tendency to decrease in a dose-dependent manner (p < 0.05). However, E-beam irradiation less than 7 kGy did not significantly affect sensory properties of the processed laver products. Irradiated laver products (DL, RL, and SL) could be screened and detected by analyzing photostimulated luminescence and thermoluminescence, respectively, from the non-irradiated ones. The overall results indicated that E-beam irradiation is effective for ensuring the improved microbial quality (< 4 log CFU/g) for the exporting processed laver products without apparent quality changes.

Keywords

E-beam irradiation Luminescence detection Microbial quality Processed laver products 

Notes

Acknowledgment

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A3B03931282).

References

  1. 1.
    Cho SM, Kim BM, Han KJ, Seo HY, Han Y, Yang EH, Kim DS (2009) Current status of the domestic processed laver market and manufacturers. Food Sci Ind 42:57–70Google Scholar
  2. 2.
    Kim KW, Hwang JH, Oh MJ, Kim MY, Choi MR, Park WM (2014) Studies on the nutritional components of commercial dried laver (Porphyra yezoensis) cultivated in Korea. Korean J Food Preserv 21:702–709CrossRefGoogle Scholar
  3. 3.
    Park SY, Song HH, Ha SD (2014) Synergistic effects of NaOCl and ultrasound combination on the reduction of Escherichia coli and Bacillus cereus in raw laver. Foodborne Pathog Dis 11:373–378CrossRefGoogle Scholar
  4. 4.
    Ahn HJ, Yook SH, Kim DH, Kim S, Byun MW (2001) Identification of radiation-resistant bacterium isolated from dried laver (Porphyra tenera). J Korean Soc Food Sci Nutr 30:193–195Google Scholar
  5. 5.
    Lee TS, Lee HJ, Byun HS, Kim JH, Park MJ, Park HY, Jung KJ (2000) Effect of heat treatment in dried lavers and modified processing. J Korean Fish Soc 33:529–532Google Scholar
  6. 6.
    Jo CR, Lee NY, Hoog SP, Kim YH, Byun MW (2004) Microbial contamination of food materials for manufacturing Korean laver roll (Kimbab) and the effect of gamma irradiation. J Food Sci Nutr 9:236–239Google Scholar
  7. 7.
    Chung HJ, Lee NY, Jo CR, Shin DH, Byun MW (2007) Use of gamma irradiation for inactivation of pathogens inoculated into Kimbab, steamed rice rolled by dried laver. Food Control 18:108–112CrossRefGoogle Scholar
  8. 8.
    Kim BR, Kim AJ, Shin JK (2013) Effect of sterilization by intense pulsed light on radiation-resistant bacterium, Micrococcus roseus. Korean J Food Sci Technol 45:248–251CrossRefGoogle Scholar
  9. 9.
    Ministry of Food and Drug Safety (MFDS) (2017) Korea food standard code. http://www.foodsafetykorea.go.kr/portal/safefoodlife/food/foodRvlv/foodRvlv.do. Accessed 26 April 2017
  10. 10.
    General administration of quality supervision, inspection and quarantine of the People’s Republic of China. http://www.aqsiq.gov.cn/LawsandRegulations/. Accessed 25 April 2017
  11. 11.
    Koksel H, Sapirstein HD, Celik S, Bushuk W (1998) Effects of gamma-irradiation of wheat on gluten proteins. J Cereal Sci 28:243–250CrossRefGoogle Scholar
  12. 12.
    FAO/WHO CODEX STAN. General codex methods for the detection of irradiated foods. http://www.fao.org/input/download/standards/377/CXS_231e.pdf. Accessed 26 April 2017
  13. 13.
    EN 13751 (2009) Foodstuffs-detection of irradiated food using photostimulated luminescence. European Committee for Standardization (CEN), BrusselsGoogle Scholar
  14. 14.
    EN 1788 (2001) Foodstuffs-thermoluminescence detection of irradiated food from which silicate minerals can be isolated. Brussel, European Committee for Standardization (CEN)Google Scholar
  15. 15.
    Lee KH, Song SH, Jeong IH (1987) Quality changes of dried lavers during processing and storage. Bull Korean Fish Soc 20:408–418Google Scholar
  16. 16.
    Mecbeth JW (1972) Carotenoids from nudibranchs. Comp Biochem Phys B 41:55–68CrossRefGoogle Scholar
  17. 17.
    Meilgaard M, Civille GV, Carr BT (2007) Overall difference tests: does a sensory difference exist between samples. Sens Eval Tech 4:63–104Google Scholar
  18. 18.
    Kim YJ, Oh HS, Kim MJ, Kim JH, Goh JB, Choi IY, Park MK (2016) Identification of electron beam-resistant bacteria in the microbial reduction of dried laver (Porphyra tenera) subjected to electron beam treatment. Korean J Food Preserv 23:139–143CrossRefGoogle Scholar
  19. 19.
    Waje CK, Jun SY, Lee YK, Kim BN, Han DH, Jo C, Kwon JH (2009) Microbial quality assessment and pathogen inactivation by electron beam and gamma irradiation of commercial seed sprouts. Food Control 20:200–204CrossRefGoogle Scholar
  20. 20.
    Ko JK, Ma YH, Song KB (2005) Effect of electron beam irradiation on microbial qualities of whole black pepper powder and commercial Sunsik. Korean J Food Sci Technol 37:308–312Google Scholar
  21. 21.
    Oh S, Shin M, Lee K, Choe E (2013) Effects of water activity on pigments in dried laver (Porphyra) during storage. Food Sci Biotechnol 22:1523–1529CrossRefGoogle Scholar
  22. 22.
    Kwon JH, Kim GR, Ahn JJ, Akram K, Bae HM, Kim CH, Kim Y, Han BS (2013) Changes in physicochemical, nutritional and hygienic properties of Chinese cabbage seeds and their sprouts on gamma and electron beam irradiation. J Food Qual 36:316–323CrossRefGoogle Scholar
  23. 23.
    Choi ID, Byun MW, Kim DH (2004) Identification of irradiation treatment of Korean susam by using thermoluminescence (TL) analysis. Food Sci Biotechnol 13:636–639Google Scholar
  24. 24.
    Kwon JH, Ahn JJ, Akram K, Son IJ, Lee SO (2013) Characterization of radiation-induced luminescence properties and free radicals for the identification of different gamma-irradiated teas. Anal Bioanal Chem 405:4225–4234CrossRefGoogle Scholar
  25. 25.
    Akram K, Ahn JJ, Kwon JH (2012) Analytical methods for the identification of irradiated foods. In: Belotserkovsky E, Ostaltsov Z (eds) Ionizing radiation: applications, sources and biological effects. Nova Science Publishers Inc, New York, pp 1–48Google Scholar
  26. 26.
    Lee J, Kausar T, Kim BK, Kwon JH (2008) Detection of γ-irradiated sesame seeds before and after roasting by analyzing photostimulated luminescence, thermoluminescence, and electron spin resonance. J Agric Food Chem 56:7184–7188CrossRefGoogle Scholar
  27. 27.
    Ahn JJ, Akram K, Lee J, Kim KS, Kwon JH (2012) Identification of a gamma-irradiated ingredient (garlic powder) in Korean barbeque sauce by thermoluminescence analysis. J Food Sci 77:C476–C480CrossRefGoogle Scholar
  28. 28.
    D’Oca MC, Bartolotta A (2010) The identification of irradiated crustaceans and evaluation of the dose by thermoluminescence: intercomparison between two methods for extracting minerals. Food Res Int 43:1255–1259CrossRefGoogle Scholar
  29. 29.
    Ahn JJ, Kim GR, Akram K, Kim JS, Kwon JH (2012) Changes in thermoluminescence properties of minerals separated from irradiated potatoes and garlic during long-term storage under different light conditions. Eur Food Res Technol 235:75–82CrossRefGoogle Scholar
  30. 30.
    An KA, Arshad MS, Jo Y, Chung N, Kwon JH (2017) E-Beam irradiation for improving the microbiological quality of smoked duck meat with minimum effects on physicochemical properties during storage. J Food Sci 82:865–872CrossRefGoogle Scholar

Copyright information

© The Korean Society for Applied Biological Chemistry 2017

Authors and Affiliations

  • Eun-Jin Lee
    • 1
    • 2
  • Gui-Ran Kim
    • 1
  • Kashif Ameer
    • 1
  • Hyun-Kyu Kyung
    • 1
  • Joong-Ho Kwon
    • 1
  1. 1.School of Food Science and BiotechnologyKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Food Analysis DivisionInstitute of Health and Environment in Daegu Metropolitan CityDaeguRepublic of Korea

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