Development of cabbage reference material for multi-residue pesticide analysis
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Cabbage reference material for pesticide multi-residue analysis was developed in accordance with the ISO Guide 35, ISO Guide 13528 and European Union Reference Laboratories-Proficiency Test standard protocols. Ten pesticides (acetamiprid, azoxystrobin, boscalid, buprofezin, carbendazim, difenoconazole, ethofenprox, imidacloprid, pyraclostrobin and tebuconazole) detected at relatively high levels in agricultural products in Korea were selected for this study. The developed material was evaluated for homogeneity and stability according to the statistical assessment method specified by international standards. Analysis of variance was carried out to calculate the within-bottle standard variation (s wb) and the between-bottle standard variation (s bb). Values of s wb and s bb varied by less than 4.7% of assigned values. Homogeneity was also assessed using Cochrane testing of outliers. All pesticides in the material were uniformly distributed within or between all bottles. Stability tests were conducted at room temperature (20–30 °C) for 12 days, under cold conditions (4–8 °C) for 40 days, under freezing conditions (− 20 °C) for 70 days and under deep freezer conditions (− 80 °C) for 234 days. Stability was evaluated based on the ISO Guide 35 statistical model, and results showed no significant decrease in stability during storage for any pesticide under any condition. We therefore conclude that the cabbage material could be used for future proficiency tests and/or validation of pesticide residue analysis.
KeywordsCabbage Multi-residue pesticide analysis Proficiency test Reference material Stability
This study was supported by Grant No. PJ010857022017 from the Rural Development Administration (RDA), Republic of Korea.
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