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Recycling of lignocellulosic waste materials to produce high-value products: single cell oil and xylitol

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Abstract

Application of microorganisms with ability of using environmental wastes such as lignocellulosic materials for converting them to high-value products is important from economical point of view. Valuable products such as single cell oil (SCO) have a high potential to be used in various industrial fields including biodiesel production. Other important products are polyalcohols like xylitol, which are applicable in the food and pharmaceutical industries. In this study, Rhodotorula yeast was isolated from the leaves of Benjamin. Afterward, SCO production was evaluated in a nitrogen-limited medium, and the obtained oil was analyzed by gas chromatography–mass spectrometery. Moreover, xylitol was produced in a media containing xylose and analyzed by high-performance liquid chromatography technique. The yeast strain was identified using polymerase chain reaction method. SCO and xylitol production was also evaluated in a medium containing lignocellulosic materials and other forestry residues. This strain produced SCO and dry biomass of 9.7 and 16.14 g/L, respectively. In addition, in a medium with 140 g/L of xylose, xylitol production was found to be 49.28 g/L. Among waste materials, lipid content of 49 % on grass hydrolysate was found to be incredible. The obtained strain was identified as Rhodotorula mucilaginosa. The results of this study show that by the isolation of yeast with the potential of making use of waste materials, high-value products can be obtained. Therefore, it can be concluded that the mentioned bioprocess has not only environmental benefits but also is very important from economical view.

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References

  • Abghari A, Chen SH (2014) Yarrowia lipolytica as an oleaginous cell factory platform for production of fatty acid-based biofuel and bioproducts. Front Energy Res 2:21. doi:10.3389/fenrg.2014.00021

    Article  Google Scholar 

  • Altamirano A, Vazquez F, De Figueroa LIC (2000) Isolation and identification of xylitol- producing yeasts from agricultural residues. Folia Microbiol (Praha) 45(3):255–258

    Article  CAS  Google Scholar 

  • Amaretti A, Raimondi S, Sala M, Roncaglia L, Lucia DM, Leonardi A, Rossi M (2010) Single cell oil of cold adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785. Microbiol Cell Fact 23:59–73

    Google Scholar 

  • Amaretti A, Raimondi S, Leonardi A, Rossi M (2012) Candida freyschussii: an oleaginous yeast producing lipids from glycerol. Chem Eng 27:139–144

    Google Scholar 

  • Benjamas CH, Louhasakul Y (2013) Industrial wastes as a promising renewable source for production of microbial lipid and direct transesterification of the lipid into biodiesel. Bioresour Technol 142:329–337

    Article  Google Scholar 

  • Beopoulos A, Mrozova Z, Thevenieau F, Dall MTL, Hapala I, Papanikplaou S, Chardot T, Nicaud JM (2008) control of lipid accumulation in the yeast Yarrowia lipolytica. Appl Environ Microbiol 74(24):7779–7789

    Article  CAS  Google Scholar 

  • Bura R, Vajzovic A, Doty SL (2012) Novel endophytic yeast Rhodotorula mucilaginosa strain PTD3I: production of xylitol and ethanol. J Ind Microbiol Biotechnol 39(7):1003–1011

    Article  CAS  Google Scholar 

  • Cheng KK, Ling HZ, Zhang JA, Ping WZ, Huang W, Ge JP, Xu JM (2010) Strain isolation and study on process parameters for xylose to xylitol bioconversion. Food Biotechnol 24(1):1606–1611

  • Dai C, Tao J, Xie F, Dai YJ, Zhao M (2007) Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. Afr J Biotechnol 6:2130–2134

    Article  CAS  Google Scholar 

  • Deak T, Chen J, Beuchat LR (2000) Molecular characterization of Yarrowialipolytica and Candida zeylanoides isolated from poultry. Appl Environ Mirobiol 66:4340–4344

    Article  CAS  Google Scholar 

  • Easterling ER, French WT, Hernandez R, Licha M (2009) The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rodotorula glutinis. Bioresour Technol 100:356–361

    Article  CAS  Google Scholar 

  • Economou CN, Aggelis G, Pavlou S, Vayenas DV (2010) Modeling of single cell oil production under nitrogen-limited and substrate inhibition condition. Biotechnol Bioeng 108(5):1049–1055

    Article  Google Scholar 

  • Economou CHN, Aggelis G, Pavlou S, Vayenas DV (2011) Single cell oil production from rice hulls hydrolysate. Bioresour Technol 102(20):9737–9742

    Article  CAS  Google Scholar 

  • EL-Batal AI, Khalaf SA (2004) Xylitol production from corn cobs hemicellulosichydrolysate by Candida tropicalis immobilized cells in hydrogel copolymer carrier. IJAB 6(6):1066–1073

    CAS  Google Scholar 

  • El-Fadaly H, El-Ahmady N, Marvan EM (2009) Single cell oil production by an oleaginous yeast strain in a low cost cultivation medium. Res J Microbiol 4(8):301–313

    Article  CAS  Google Scholar 

  • Enshaeieh M, Abdoli A, Nahvi I, Madani M (2012) Bioconversion of different carbon sources into microbial oil and biodiesel using oleaginous yeasts. J Biol Today’s World 1(2):82–92

    Google Scholar 

  • Enshaeieh M, Abdoli A, Nahvi I (2013a) Medium optimization for biotechnological production of single cell oil using Yarrowia lipolytica M7 and Candida sp. JCMR 5(1):17–23

    Google Scholar 

  • Enshaeieh M, Abdoli A, Nahvi I, Madani M (2013b) Selection and optimization of single cell oil production from Rodotorula 110 using environmental waste as substrate. JCMR 4(2):1–10

    Google Scholar 

  • Enshaeieh M, Nahvi I, Madani M (2014) Improving microbial oil production with standard and native oleaginous yeasts by using Taguchi design. Int J Environ Sci Technol 11(3):597–604

    Article  CAS  Google Scholar 

  • Fakas S, Papanikolaou S, Galiotou-Panayotou M, Komaitis M, Aggelis G (2008) Biochemistry and biotechnology of single cell oil. University of Patras, pp 38–60

  • Fei Q, Chang HN, Shang L, Choi JD, Kim N, Kang J (2010) The effect of volatile fatty acid as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production. Bioresour Technol 102(3):2695–2701

    Article  Google Scholar 

  • Gong Z, Shen H, Wang Q, Yang X, Xie H, Zhao Z (2013) Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process. Biotechnol Biofiuels 6:36–54

    Article  CAS  Google Scholar 

  • Granstrom T (2002) Biotechnological Production of Xylitol with Candida Yeasts. Available in the electronic publications archive of Aalto University. ISBN 951-22-5992-3

  • Granstrom T, Izumori K, Leisola M (2007) A rare sugar xylitol PartI: the biochemistry and biosynthesis of xylitol. Appl Microbiol Biotechnol 74(2):277–281

    Article  Google Scholar 

  • Huang YT, Su CP (2014) High lipid content and productivity of microalgae cultivating under elevated carbon dioxide. Int J Environ Sci Technol 11(3):703–710

    Article  CAS  Google Scholar 

  • Huang C, Zong MH, Wu H, Liu QP (2009) Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour Technol 100:4535–4538

    Article  CAS  Google Scholar 

  • Huang CH, Chen XF, Xiong L, Chen X, Ma L (2012) Oil production by the yeast Trichosporon dermatis cultured in enzymatic hydrolysates of corncobs. Bioresour Technol 110:711–714

    Article  CAS  Google Scholar 

  • Huang CH, Chen XF, Xiong L, Chen XD, Ma L, Chen Y (2013) Single cell oil production from low-cost substrates: the possibility and potential of its industrialization. Biotechnol Adv 31(2):129–139

    Article  CAS  Google Scholar 

  • Ikeuchi T, Azuma M, kato J, Ooshima H (1999) Screening of microorganisms for xylitol production and fermentation behavior in high concentrations of xylose. Biomass Bioenerg 16:333–339

    Article  CAS  Google Scholar 

  • Karatay SE, Donmez G (2010) Improving the lipid accumulation properties of the yeast cells for biodiesel production using molasses. Bioresour Technol 101(20):7988–7990

    Article  CAS  Google Scholar 

  • Katre G, Joshi Ch, Khot M, Zinjarde S, Ravikumar A (2012) Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel. AMB Express 2:36–55

    Article  Google Scholar 

  • Khot M, Kamat S, Zinjarde S, Pant A, Chopade B, Ravikumar A (2012) Single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel. Microb Cell Fact 11(71):1–13

    Google Scholar 

  • Kraisintu P, Yongmanitchai W, Limtong S (2010) Selection and optimization for lipid production of a newly isolated oleaginous yeast, Rdodosporidium toruloides DMKU3-TK16. Kasetsart J (Nat Sci) 44:436–445

    CAS  Google Scholar 

  • Leesing R, Baojungharn R (2011) Microbial Oil production by isolated oleaginous yeast Torulaspora globosa YU5/2. WASET 76:799–803

    Google Scholar 

  • Li YH, Liu B, Sun Y, Zhao ZB, Bai FW (2005) Screening of oleaginous yeasts for broad-spectrum carbohydrates assimilation capacity. Program Biotechnol 25:39–43

    Google Scholar 

  • Li Q, Du W, Liu D (2008) Perspective ofmicrobial oils for biodiesel production. Appl Microbiol Biotechnol 80:749–756

    Article  CAS  Google Scholar 

  • Liu GY, Yuan S, Dai CC (2004) Factors affecting γ-linoleic acid content in fermented glutinous rice brewed by Rhizopus sp. Food Microbiol 21(3):299–304

  • Liu GQ, Lin QL, Jin XC, Wang XL, Zhao Y (2010) Screening and fermentation optimization of microbial lipid-producing molds from forest soils. Afr J microbiol Res 4(14):1462–1468

    CAS  Google Scholar 

  • Mattanna P, Rosa PD, Poli J, Richards NSPS, Daboit TC, Scroferneker ML, Pastore APW, Corcao G, Bertoldi FC, Deschamps FC, Valente P (2014) Lipid profile and antimicrobial activity of microbial oils from 16 oleaginous yeasts isolated from artisanal cheese. Revista Brasileira de Biociências 12(2):121–126

    Google Scholar 

  • Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2009) Biodiesel production from oleaginous microorganisms. Renew Energ 34:1–5

    Article  Google Scholar 

  • Muniraj IK, Xiao L, Hu Z, Zhan X, Shi J (2013) Microbial lipid production from potato processing wastewater using oleaginous filamentous fungi Aspergillus oryzae. Water Res 47(10):3477–3483

    Article  CAS  Google Scholar 

  • Mussatto SI, Roberto IC (2008) Establishment of the optimum initial xylose concentration and nutritional supplementation of brewer’s spent grain hydrolysate for xylitol production by Candida guilliermondii. Process Biochem 43:540–546

    Article  CAS  Google Scholar 

  • Pan LX, Yang DF, Shao L, Li W, Chen GG, Liang ZQ (2009) Isolation of oleaginous yeast from the soil and studies of their lipid-producing capacities. Food Technol Biotechnol 47(2):215–220

    CAS  Google Scholar 

  • Papanikolaou S (2008) Current Topics on Bioprocesses in Food Industry, asiatech publishers Inc. 1, 3-Propanediol and citric acid production from glycerol containing waste discharged after bio-diesel manufacturing process. pp 381–399

  • Papanikolaou S, Aggelis G (2003) Modeling lipid accumulation and degradation in Yarrowia lipolytica cultivated on industrial fats. Curr Microbiol 46(6):398–402

    Article  CAS  Google Scholar 

  • Papanikolaou S, Aggelis G (2011) Lipids of oleaginous yeasts. Part II: technology and potential applications. Eur J Lipid Sci Technol 113(8):1052–1073

    Google Scholar 

  • Papanikolaou S, Chevalot I, Komaitis M, Marc I, Aggelis G (2001) Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures. Appl Microbiol Biotechnol 58:308–312

    Article  Google Scholar 

  • Pirozzi D, Ausiello A, yousuf A, Zuccaro G, Toscano G (2014) Exploitation of oleaginous yeasts for the production of microbial oils from agricultural biomass. Chem Eng 37:469–474

    Google Scholar 

  • Rao RS, Bhadra B, Shivaji S (2007) Isolation and characterization of xylitol- producing yeasts from the gutofcolleopteran insects. Curr Microbiol 55(5):441–446

    Article  CAS  Google Scholar 

  • Rao RS, Jyothi CP, Rao LV (2008) Biothechnological production of xylitol by mutant Candida tropicalis OMV5: process optimization using statistical approach. IJBT 7:218–224

    CAS  Google Scholar 

  • Raschke D, Knorr D (2009) Rapid monitoring of cell size, vitality and lipid droplet development in oleaginous yeast Waltomyces lipofer. J Microbiol Methods 79:178–183

    Article  CAS  Google Scholar 

  • Ratledge C (2002) Regulation of lipid accumulation in oleaginous micro-organisms. Biochem Soc Trans 30(6):1047–1050

    Article  CAS  Google Scholar 

  • Ratledge C (2005) Single cell oil for the 21st century, Cohen Z, Single Cell Oils, 978-1-893997-80-6. University of Hull, Hull, pp 1–20

    Google Scholar 

  • Sandhya M, Aravind J, Kanmani P (2013) Production of polyhydroxyalkanoates from Ralstonia eutropha using paddy straw as cheap substrate. Int J Environ Sci Technol 10(1):47–54

    Article  CAS  Google Scholar 

  • Santamauro F, Whiffin FM, Scott RJ, Chuck CHJ (2014) Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources. Biotechnol biofuels 7(34). doi:10.1186/1754-6834-7-34

  • Saxena RK, Anand P, Saran S, Isar J (2009) Microbial production of 1, 3-propanediol: recent developments and emerging opportunities. Biotechnol Adv 27:895–913

    Article  CAS  Google Scholar 

  • Schulze I, Hansen S, Großhans S, Rudszuck TH, Ochsenreither K, Syldatk CH, Neumann A (2014) Characterization of newly isolated oleaginous yeasts: Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis. AMB Express 4(24). doi 10.1186/s13568-014-0024-0

  • Sriwongchai S, Pokethitiyook P, Kruatrachue M, Bajwa PK, Lee H (2013) Screening of selected oleaginous yeasts for lipid production from glycerol and some factors which affect lipid production by Yarrowia lipolytica strains. J Microbiol Biotechnol Food Sci 2(5):2344–2348

    CAS  Google Scholar 

  • Susan HD, Maugeri F (2014) Prediction of quality properties for biodiesel production by oleaginous yeast cultivated in pure and raw glycerol. Chem Eng 37:465–468

    Google Scholar 

  • Syed MA, Singh SK, Pandey A, Kanjilal S, Prasad RBN (2006) Effects of various process parameters on the production of α-Linolenic acid in submerged fermentation. Food Technol Biotechnol 44:282–287

    Google Scholar 

  • Tanimura A, Takashima M, Sugita T, Endoh R, Kikukawa M, Yamaguchi SH, Sakuradani E, Ogawa J, Ohkuma M, Shima J (2014) Cryptococcus terricola is a promising oleaginous yeast for biodiesel production from starch through consolidated bioprocessing. Sci Rep 4:4776

    Article  Google Scholar 

  • Tao J, Dai CC, Yang QY, Guan XY, Shao WL (2010) Production of biodiesel with acid hydrolysate of populus euramevicana cv leaves by Rhodotorula glutinis. Int J Green Energy 7:387–396

    Article  CAS  Google Scholar 

  • Toyoda T, Ohtaguchi K (2009) Xylitol production from lactose by biotransformation. J Biochem Tech 2(1):126–132

    CAS  Google Scholar 

  • Tsigie YA, Wang CY, Truong CHT, Ju YH (2011) Lipid production from Yarrowia lipolytica Po1 g grown in sugarcane bagasse hydrolysate. Bioresour Technol 102(19):9216–9222

    Article  CAS  Google Scholar 

  • Vakhlu J, Kour A (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 9:69–85

    Article  CAS  Google Scholar 

  • Vijayakumar S, Kumutha K, Santhana Krishnan P, Gopal H (2010) Effect of carbon sources on lipid and biomass production by oleaginous yeast cultures. MAJ 97(1/3):62–64

    Google Scholar 

  • Wu Q, Miao XL (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour Technol 97:841–846

  • Yu XC, Zheng YB, Dorgan KM, Chen SL (2011) Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 102:6134–6140

    Article  CAS  Google Scholar 

  • Zagustina NA, Rodionova NA, Mestechkina NM, Shcherbukhin VD, Bezborodov AM (2001) Xylitol production by a culture of Candida guilliermondii2581. Appl Microbiol Biotechnol 37(5):489–492

    CAS  Google Scholar 

  • Zhao X, Peng F, Du W, Liu C, Liu D (2012) Effects of some inhibitors on the growth and lipid accumulation of oleaginous yeast Rhodosporidium toruloides and preparation of biodiesel by enzymatic transesterification of the lipid. Bioprocess Biosyst Eng 35(6):993–1004

    Article  CAS  Google Scholar 

  • Zheng Y, Yu X, Zeng J, Chen Sh (2012) Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw. Biotechnol Biofuels 5(50):1750–1754

    Google Scholar 

  • Zhu LY, Zong MH, Wu H (2008) Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Bioresour Technol 99(16):7881–7885

    Article  CAS  Google Scholar 

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Acknowledgments

This paper was extracted from research project with code of 92062, and the authors wish to extend their sincere gratitude to all who have supported this work, especially Young Researchers and Elite Club of Flavarjan Islamic Azad University.

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Authors and Affiliations

  1. Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

    M. Enshaeieh & A. Abdoli

  2. Assistant Professor of Medical Mycology, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

    M. Madani

  3. Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran

    M. Bayat

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  1. M. Enshaeieh
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Correspondence to M. Enshaeieh.

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Enshaeieh, M., Abdoli, A., Madani, M. et al. Recycling of lignocellulosic waste materials to produce high-value products: single cell oil and xylitol. Int. J. Environ. Sci. Technol. 12, 837–846 (2015). https://doi.org/10.1007/s13762-014-0687-8

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