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Recycling of lignocellulosic waste materials to produce high-value products: single cell oil and xylitol

Original Paper

Abstract

Application of microorganisms with ability of using environmental wastes such as lignocellulosic materials for converting them to high-value products is important from economical point of view. Valuable products such as single cell oil (SCO) have a high potential to be used in various industrial fields including biodiesel production. Other important products are polyalcohols like xylitol, which are applicable in the food and pharmaceutical industries. In this study, Rhodotorula yeast was isolated from the leaves of Benjamin. Afterward, SCO production was evaluated in a nitrogen-limited medium, and the obtained oil was analyzed by gas chromatography–mass spectrometery. Moreover, xylitol was produced in a media containing xylose and analyzed by high-performance liquid chromatography technique. The yeast strain was identified using polymerase chain reaction method. SCO and xylitol production was also evaluated in a medium containing lignocellulosic materials and other forestry residues. This strain produced SCO and dry biomass of 9.7 and 16.14 g/L, respectively. In addition, in a medium with 140 g/L of xylose, xylitol production was found to be 49.28 g/L. Among waste materials, lipid content of 49 % on grass hydrolysate was found to be incredible. The obtained strain was identified as Rhodotorula mucilaginosa. The results of this study show that by the isolation of yeast with the potential of making use of waste materials, high-value products can be obtained. Therefore, it can be concluded that the mentioned bioprocess has not only environmental benefits but also is very important from economical view.

Keywords

Rhodotorula mucilaginosa Environmental wastes Grass hydrolysate Forestry residues 

Notes

Acknowledgments

This paper was extracted from research project with code of 92062, and the authors wish to extend their sincere gratitude to all who have supported this work, especially Young Researchers and Elite Club of Flavarjan Islamic Azad University.

References

  1. Abghari A, Chen SH (2014) Yarrowia lipolytica as an oleaginous cell factory platform for production of fatty acid-based biofuel and bioproducts. Front Energy Res 2:21. doi: 10.3389/fenrg.2014.00021 CrossRefGoogle Scholar
  2. Altamirano A, Vazquez F, De Figueroa LIC (2000) Isolation and identification of xylitol- producing yeasts from agricultural residues. Folia Microbiol (Praha) 45(3):255–258CrossRefGoogle Scholar
  3. Amaretti A, Raimondi S, Sala M, Roncaglia L, Lucia DM, Leonardi A, Rossi M (2010) Single cell oil of cold adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785. Microbiol Cell Fact 23:59–73Google Scholar
  4. Amaretti A, Raimondi S, Leonardi A, Rossi M (2012) Candida freyschussii: an oleaginous yeast producing lipids from glycerol. Chem Eng 27:139–144Google Scholar
  5. Benjamas CH, Louhasakul Y (2013) Industrial wastes as a promising renewable source for production of microbial lipid and direct transesterification of the lipid into biodiesel. Bioresour Technol 142:329–337CrossRefGoogle Scholar
  6. Beopoulos A, Mrozova Z, Thevenieau F, Dall MTL, Hapala I, Papanikplaou S, Chardot T, Nicaud JM (2008) control of lipid accumulation in the yeast Yarrowia lipolytica. Appl Environ Microbiol 74(24):7779–7789CrossRefGoogle Scholar
  7. Bura R, Vajzovic A, Doty SL (2012) Novel endophytic yeast Rhodotorula mucilaginosa strain PTD3I: production of xylitol and ethanol. J Ind Microbiol Biotechnol 39(7):1003–1011CrossRefGoogle Scholar
  8. Cheng KK, Ling HZ, Zhang JA, Ping WZ, Huang W, Ge JP, Xu JM (2010) Strain isolation and study on process parameters for xylose to xylitol bioconversion. Food Biotechnol 24(1):1606–1611Google Scholar
  9. Dai C, Tao J, Xie F, Dai YJ, Zhao M (2007) Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. Afr J Biotechnol 6:2130–2134CrossRefGoogle Scholar
  10. Deak T, Chen J, Beuchat LR (2000) Molecular characterization of Yarrowialipolytica and Candida zeylanoides isolated from poultry. Appl Environ Mirobiol 66:4340–4344CrossRefGoogle Scholar
  11. Easterling ER, French WT, Hernandez R, Licha M (2009) The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rodotorula glutinis. Bioresour Technol 100:356–361CrossRefGoogle Scholar
  12. Economou CN, Aggelis G, Pavlou S, Vayenas DV (2010) Modeling of single cell oil production under nitrogen-limited and substrate inhibition condition. Biotechnol Bioeng 108(5):1049–1055CrossRefGoogle Scholar
  13. Economou CHN, Aggelis G, Pavlou S, Vayenas DV (2011) Single cell oil production from rice hulls hydrolysate. Bioresour Technol 102(20):9737–9742CrossRefGoogle Scholar
  14. EL-Batal AI, Khalaf SA (2004) Xylitol production from corn cobs hemicellulosichydrolysate by Candida tropicalis immobilized cells in hydrogel copolymer carrier. IJAB 6(6):1066–1073Google Scholar
  15. El-Fadaly H, El-Ahmady N, Marvan EM (2009) Single cell oil production by an oleaginous yeast strain in a low cost cultivation medium. Res J Microbiol 4(8):301–313CrossRefGoogle Scholar
  16. Enshaeieh M, Abdoli A, Nahvi I, Madani M (2012) Bioconversion of different carbon sources into microbial oil and biodiesel using oleaginous yeasts. J Biol Today’s World 1(2):82–92Google Scholar
  17. Enshaeieh M, Abdoli A, Nahvi I (2013a) Medium optimization for biotechnological production of single cell oil using Yarrowia lipolytica M7 and Candida sp. JCMR 5(1):17–23Google Scholar
  18. Enshaeieh M, Abdoli A, Nahvi I, Madani M (2013b) Selection and optimization of single cell oil production from Rodotorula 110 using environmental waste as substrate. JCMR 4(2):1–10Google Scholar
  19. Enshaeieh M, Nahvi I, Madani M (2014) Improving microbial oil production with standard and native oleaginous yeasts by using Taguchi design. Int J Environ Sci Technol 11(3):597–604CrossRefGoogle Scholar
  20. Fakas S, Papanikolaou S, Galiotou-Panayotou M, Komaitis M, Aggelis G (2008) Biochemistry and biotechnology of single cell oil. University of Patras, pp 38–60Google Scholar
  21. Fei Q, Chang HN, Shang L, Choi JD, Kim N, Kang J (2010) The effect of volatile fatty acid as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production. Bioresour Technol 102(3):2695–2701CrossRefGoogle Scholar
  22. Gong Z, Shen H, Wang Q, Yang X, Xie H, Zhao Z (2013) Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process. Biotechnol Biofiuels 6:36–54CrossRefGoogle Scholar
  23. Granstrom T (2002) Biotechnological Production of Xylitol with Candida Yeasts. Available in the electronic publications archive of Aalto University. ISBN 951-22-5992-3Google Scholar
  24. Granstrom T, Izumori K, Leisola M (2007) A rare sugar xylitol PartI: the biochemistry and biosynthesis of xylitol. Appl Microbiol Biotechnol 74(2):277–281CrossRefGoogle Scholar
  25. Huang YT, Su CP (2014) High lipid content and productivity of microalgae cultivating under elevated carbon dioxide. Int J Environ Sci Technol 11(3):703–710CrossRefGoogle Scholar
  26. Huang C, Zong MH, Wu H, Liu QP (2009) Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour Technol 100:4535–4538CrossRefGoogle Scholar
  27. Huang CH, Chen XF, Xiong L, Chen X, Ma L (2012) Oil production by the yeast Trichosporon dermatis cultured in enzymatic hydrolysates of corncobs. Bioresour Technol 110:711–714CrossRefGoogle Scholar
  28. Huang CH, Chen XF, Xiong L, Chen XD, Ma L, Chen Y (2013) Single cell oil production from low-cost substrates: the possibility and potential of its industrialization. Biotechnol Adv 31(2):129–139CrossRefGoogle Scholar
  29. Ikeuchi T, Azuma M, kato J, Ooshima H (1999) Screening of microorganisms for xylitol production and fermentation behavior in high concentrations of xylose. Biomass Bioenerg 16:333–339CrossRefGoogle Scholar
  30. Karatay SE, Donmez G (2010) Improving the lipid accumulation properties of the yeast cells for biodiesel production using molasses. Bioresour Technol 101(20):7988–7990CrossRefGoogle Scholar
  31. Katre G, Joshi Ch, Khot M, Zinjarde S, Ravikumar A (2012) Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel. AMB Express 2:36–55CrossRefGoogle Scholar
  32. Khot M, Kamat S, Zinjarde S, Pant A, Chopade B, Ravikumar A (2012) Single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel. Microb Cell Fact 11(71):1–13Google Scholar
  33. Kraisintu P, Yongmanitchai W, Limtong S (2010) Selection and optimization for lipid production of a newly isolated oleaginous yeast, Rdodosporidium toruloides DMKU3-TK16. Kasetsart J (Nat Sci) 44:436–445Google Scholar
  34. Leesing R, Baojungharn R (2011) Microbial Oil production by isolated oleaginous yeast Torulaspora globosa YU5/2. WASET 76:799–803Google Scholar
  35. Li YH, Liu B, Sun Y, Zhao ZB, Bai FW (2005) Screening of oleaginous yeasts for broad-spectrum carbohydrates assimilation capacity. Program Biotechnol 25:39–43Google Scholar
  36. Li Q, Du W, Liu D (2008) Perspective ofmicrobial oils for biodiesel production. Appl Microbiol Biotechnol 80:749–756CrossRefGoogle Scholar
  37. Liu GY, Yuan S, Dai CC (2004) Factors affecting γ-linoleic acid content in fermented glutinous rice brewed by Rhizopus sp. Food Microbiol 21(3):299–304Google Scholar
  38. Liu GQ, Lin QL, Jin XC, Wang XL, Zhao Y (2010) Screening and fermentation optimization of microbial lipid-producing molds from forest soils. Afr J microbiol Res 4(14):1462–1468Google Scholar
  39. Mattanna P, Rosa PD, Poli J, Richards NSPS, Daboit TC, Scroferneker ML, Pastore APW, Corcao G, Bertoldi FC, Deschamps FC, Valente P (2014) Lipid profile and antimicrobial activity of microbial oils from 16 oleaginous yeasts isolated from artisanal cheese. Revista Brasileira de Biociências 12(2):121–126Google Scholar
  40. Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2009) Biodiesel production from oleaginous microorganisms. Renew Energ 34:1–5CrossRefGoogle Scholar
  41. Muniraj IK, Xiao L, Hu Z, Zhan X, Shi J (2013) Microbial lipid production from potato processing wastewater using oleaginous filamentous fungi Aspergillus oryzae. Water Res 47(10):3477–3483CrossRefGoogle Scholar
  42. Mussatto SI, Roberto IC (2008) Establishment of the optimum initial xylose concentration and nutritional supplementation of brewer’s spent grain hydrolysate for xylitol production by Candida guilliermondii. Process Biochem 43:540–546CrossRefGoogle Scholar
  43. Pan LX, Yang DF, Shao L, Li W, Chen GG, Liang ZQ (2009) Isolation of oleaginous yeast from the soil and studies of their lipid-producing capacities. Food Technol Biotechnol 47(2):215–220Google Scholar
  44. Papanikolaou S (2008) Current Topics on Bioprocesses in Food Industry, asiatech publishers Inc. 1, 3-Propanediol and citric acid production from glycerol containing waste discharged after bio-diesel manufacturing process. pp 381–399Google Scholar
  45. Papanikolaou S, Aggelis G (2003) Modeling lipid accumulation and degradation in Yarrowia lipolytica cultivated on industrial fats. Curr Microbiol 46(6):398–402CrossRefGoogle Scholar
  46. Papanikolaou S, Aggelis G (2011) Lipids of oleaginous yeasts. Part II: technology and potential applications. Eur J Lipid Sci Technol 113(8):1052–1073Google Scholar
  47. Papanikolaou S, Chevalot I, Komaitis M, Marc I, Aggelis G (2001) Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures. Appl Microbiol Biotechnol 58:308–312CrossRefGoogle Scholar
  48. Pirozzi D, Ausiello A, yousuf A, Zuccaro G, Toscano G (2014) Exploitation of oleaginous yeasts for the production of microbial oils from agricultural biomass. Chem Eng 37:469–474Google Scholar
  49. Rao RS, Bhadra B, Shivaji S (2007) Isolation and characterization of xylitol- producing yeasts from the gutofcolleopteran insects. Curr Microbiol 55(5):441–446CrossRefGoogle Scholar
  50. Rao RS, Jyothi CP, Rao LV (2008) Biothechnological production of xylitol by mutant Candida tropicalis OMV5: process optimization using statistical approach. IJBT 7:218–224Google Scholar
  51. Raschke D, Knorr D (2009) Rapid monitoring of cell size, vitality and lipid droplet development in oleaginous yeast Waltomyces lipofer. J Microbiol Methods 79:178–183CrossRefGoogle Scholar
  52. Ratledge C (2002) Regulation of lipid accumulation in oleaginous micro-organisms. Biochem Soc Trans 30(6):1047–1050CrossRefGoogle Scholar
  53. Ratledge C (2005) Single cell oil for the 21st century, Cohen Z, Single Cell Oils, 978-1-893997-80-6. University of Hull, Hull, pp 1–20Google Scholar
  54. Sandhya M, Aravind J, Kanmani P (2013) Production of polyhydroxyalkanoates from Ralstonia eutropha using paddy straw as cheap substrate. Int J Environ Sci Technol 10(1):47–54CrossRefGoogle Scholar
  55. Santamauro F, Whiffin FM, Scott RJ, Chuck CHJ (2014) Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources. Biotechnol biofuels 7(34). doi: 10.1186/1754-6834-7-34
  56. Saxena RK, Anand P, Saran S, Isar J (2009) Microbial production of 1, 3-propanediol: recent developments and emerging opportunities. Biotechnol Adv 27:895–913CrossRefGoogle Scholar
  57. Schulze I, Hansen S, Großhans S, Rudszuck TH, Ochsenreither K, Syldatk CH, Neumann A (2014) Characterization of newly isolated oleaginous yeasts: Cryptococcus podzolicus, Trichosporon porosum and Pichia segobiensis. AMB Express 4(24). doi  10.1186/s13568-014-0024-0
  58. Sriwongchai S, Pokethitiyook P, Kruatrachue M, Bajwa PK, Lee H (2013) Screening of selected oleaginous yeasts for lipid production from glycerol and some factors which affect lipid production by Yarrowia lipolytica strains. J Microbiol Biotechnol Food Sci 2(5):2344–2348Google Scholar
  59. Susan HD, Maugeri F (2014) Prediction of quality properties for biodiesel production by oleaginous yeast cultivated in pure and raw glycerol. Chem Eng 37:465–468Google Scholar
  60. Syed MA, Singh SK, Pandey A, Kanjilal S, Prasad RBN (2006) Effects of various process parameters on the production of α-Linolenic acid in submerged fermentation. Food Technol Biotechnol 44:282–287Google Scholar
  61. Tanimura A, Takashima M, Sugita T, Endoh R, Kikukawa M, Yamaguchi SH, Sakuradani E, Ogawa J, Ohkuma M, Shima J (2014) Cryptococcus terricola is a promising oleaginous yeast for biodiesel production from starch through consolidated bioprocessing. Sci Rep 4:4776CrossRefGoogle Scholar
  62. Tao J, Dai CC, Yang QY, Guan XY, Shao WL (2010) Production of biodiesel with acid hydrolysate of populus euramevicana cv leaves by Rhodotorula glutinis. Int J Green Energy 7:387–396CrossRefGoogle Scholar
  63. Toyoda T, Ohtaguchi K (2009) Xylitol production from lactose by biotransformation. J Biochem Tech 2(1):126–132Google Scholar
  64. Tsigie YA, Wang CY, Truong CHT, Ju YH (2011) Lipid production from Yarrowia lipolytica Po1 g grown in sugarcane bagasse hydrolysate. Bioresour Technol 102(19):9216–9222CrossRefGoogle Scholar
  65. Vakhlu J, Kour A (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 9:69–85CrossRefGoogle Scholar
  66. Vijayakumar S, Kumutha K, Santhana Krishnan P, Gopal H (2010) Effect of carbon sources on lipid and biomass production by oleaginous yeast cultures. MAJ 97(1/3):62–64Google Scholar
  67. Wu Q, Miao XL (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour Technol 97:841–846Google Scholar
  68. Yu XC, Zheng YB, Dorgan KM, Chen SL (2011) Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 102:6134–6140CrossRefGoogle Scholar
  69. Zagustina NA, Rodionova NA, Mestechkina NM, Shcherbukhin VD, Bezborodov AM (2001) Xylitol production by a culture of Candida guilliermondii2581. Appl Microbiol Biotechnol 37(5):489–492Google Scholar
  70. Zhao X, Peng F, Du W, Liu C, Liu D (2012) Effects of some inhibitors on the growth and lipid accumulation of oleaginous yeast Rhodosporidium toruloides and preparation of biodiesel by enzymatic transesterification of the lipid. Bioprocess Biosyst Eng 35(6):993–1004CrossRefGoogle Scholar
  71. Zheng Y, Yu X, Zeng J, Chen Sh (2012) Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw. Biotechnol Biofuels 5(50):1750–1754Google Scholar
  72. Zhu LY, Zong MH, Wu H (2008) Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Bioresour Technol 99(16):7881–7885CrossRefGoogle Scholar

Copyright information

© Islamic Azad University (IAU) 2015

Authors and Affiliations

  1. 1.Young Researchers and Elite Club, Falavarjan BranchIslamic Azad UniversityIsfahanIran
  2. 2.Assistant Professor of Medical Mycology, Department of Microbiology, Falavarjan BranchIslamic Azad UniversityIsfahanIran
  3. 3.Department of Pathobiology, Faculty of Veterinary Specialized SciencesIslamic Azad University, Science and Research BranchTehranIran

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