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Covalent immobilization of xylanase from Thermomyces lanuginosus on aminated superparamagnetic graphene oxide nanocomposite

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Abstract

Today, immobilization of enzymes has been extensively considered in a wide variety of applications in industries. Xylanase is a hemicellulotic enzyme with effective usages that has attracted the attention of researchers. In this work, xylanase from Thermomyces lanuginosus was covalently immobilized on modified graphene oxide nanosheets (NGO) via cyanuric chloride linker. The magnetic nanoparticles were perched on NGO to facilitate separation of nanocarrier from the reaction media. To decrease the steric hindrance and allow the high-molecular weight xylan as a substrate to approach the active site of the immobilized xylanase, polyethylene glycol bis-amine was used as a spacer. Various techniques such as TEM, SEM, FTIR, VSM and TGA were applied to characterize the structure of synthetic nanocomposite. The amount of immobilized xylanase was quantified by Bradford assay, and the immobilization efficiency was determined about 67%. The results showed that thermal stability, pH stability, reusability and storage stability were improved for immobilized enzyme and the kinetic parameters, \({K_m}\) and \({v_{\hbox{max} }}\) values, were increased, suggesting the great potential of the functionalized NGO as a novel nanocarrier in bio-industry applications.

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Acknowledgements

The financial supports of Tehran Science and Research Branch of Islamic Azad University, Research Council of the University of Isfahan and Iran National Science Foundation (Grant no. 96007105) are kindly acknowledged.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Vajihe Mehnati-Najafabadi & Akram Eidi

  2. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, 81746-73441, Iran

    Asghar Taheri-Kafrani

  3. Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Iran

    Abdol-Khalegh Bordbar

Authors
  1. Vajihe Mehnati-Najafabadi
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  2. Asghar Taheri-Kafrani
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Correspondence to Asghar Taheri-Kafrani or Abdol-Khalegh Bordbar.

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Mehnati-Najafabadi, V., Taheri-Kafrani, A., Bordbar, AK. et al. Covalent immobilization of xylanase from Thermomyces lanuginosus on aminated superparamagnetic graphene oxide nanocomposite. J IRAN CHEM SOC 16, 21–31 (2019). https://doi.org/10.1007/s13738-018-1477-x

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