Baccharis trimera (carqueja) promotes gastroprotection on ethanol-induced acute gastric ulcer


One of the factors that can promote gastric ulcer is the acute and/or chronic consumption of ethanol. One of the most used therapeutic measures is the use of proton pump inhibitor, like omeprazole. However, these drugs have several side effects, making it necessary to search for new alternatives. To investigate the gastroprotective effect of Baccharis trimera (carqueja) we used an ethanol-induced acute gastric ulcer model. For this, gastric ulcer was induced in rats using absolute ethanol for 2 consecutive days. After analysis of the stomach, an increase in oxidative stress was observed, as well as the reduction of the antioxidant defense. In addition, we observed in histology that there was an increase in inflammatory cells, necrosis, hemorrhage and hyperemia. Treatment with B. trimera was able to decrease oxidative damage as well as improve antioxidant defense. There was also a decrease in inflammation, collagen area and hemorrhage. All the beneficial effects promoted by carqueja were similar to those found by the positive controls used in our study (vitamin C and omeprazole). Analyzing our data, we can conclude that B. trimera presents a gastroprotective effect and should be further studied for its application in gastric ulcer therapy.

Graphical abstract

After 2 days of consumption of absolute ethanol, the animals' stomachs showed an increase in oxidative stress, characterized by reduction of oxidized glutathione (GSSG) and increase of TBARS and carbonylated protein. This situation led to stomach inflammation, necrosis, hemorrhage and hyperemia. These was minimized by the consumption of Baccharis trimera extracts (Bt), since it was able to decrease oxidative damage and increasing GSSG, reduced glutathione (GSH) and catalase (CAT). In addition, there was decreased inflammation, hemorrhage and collagen area after Bt consumption. GR reductase gluthione, GPx peroxidase glutathione. Bt in green: stimulation; Bt in red: inhibits.

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Fig. 1
Fig. 2
Fig. 3



Baccharis trimera


Oxidized glutathione


Reduced glutathione


Glutathione peroxidase


Glutathione reductase




Superoxide dismutase


Control group


Ethanolic group


Hydroethanolic extract


Aqueous extract






Thiobarbituric acid reactive substances


Proton pump inhibitor


  1. Aebi H (1984) Catalase in vitro. Method Enzymol 105:121–126

    CAS  Article  Google Scholar 

  2. Bi WP (2014) Efficacy and safety of herbal medicines in treating gastric ulcer: a review. World J Gastroenterol 20(45):17020

    Article  Google Scholar 

  3. Biondo TMA, Tanae MM, Coletta ED, Lima-Landman MTR, Lapa AJ, Souccar C (2011) Antisecretory actions of Baccharis trimera (Less.) DC aqueous extract and isolated compounds: analysis of underlying mechanisms. J Ethnopharmacol 136:368–373

    CAS  Article  Google Scholar 

  4. Byeon S, Oh J, Lim JS, Lee JS, Kim JS (2018) Protective effects of dioscorea batatas flesh and peel extracts against ethanol-induced gastric ulcer in mice. Nutrition 10:1680–1694

    Google Scholar 

  5. Cardi M, Muttillo IA, Amadori L (1997) Intravenous omeprazole versus ranitidine in the treatment of hemorrhagic duodenal ulcer: a prospective randomized study. Ann Chir 51(2):136–139

    CAS  PubMed  Google Scholar 

  6. de Araújo GR, Rabelo ACS, Meira JS, Rossoni-Júnior JV, de Castro-Borges W, Guerra-Sá R, Batista MA, Silveira-Lemos D, Souza GHB, Brandão GC, Chaves MM, Costa DC (2016) Baccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47phox phosphorylation of NADPH oxidase in SK Hep-1 cells. Exp Biol Med.

    Article  Google Scholar 

  7. Demertzis K, Polymeros D, Emmanuel T, Triantafyllou K, Tassios P, Ladas SD (2006) Omeprazole maintenance therapy prevents recurrent ulcer bleeding after surgery for duodenal ulcer. World J Gastroenterol 12(5):791–795

    CAS  Article  Google Scholar 

  8. Dias LFT, Melo ES, Hernandes LS, Bacchi EM (2009) Atividades antiúlcera e antioxidante Baccharis trimera (Less) DC (Asteraceae). Ver Bras Farmacog 19:309–314

    Article  Google Scholar 

  9. Fazalda A, Quraisiah A, Azlina MFN (2018) Antiulcer effect of honey in nonsteroidal anti-inflammatory drugs induced gastric ulcer model in rats: a systematic review. Evid Based Complement Alternat Med.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Franke A, Teyssen S, Singer MV (2005) Alcohol-related diseases of the esophagus and stomach. Dig Dis 23:204–213

    Article  Google Scholar 

  11. GBD (2015) Mortality and causes of death collaborators. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990–2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet 385:117–171

    Article  Google Scholar 

  12. Harsha C, Banik K, Bordoloi D, Kunnumakkara AB (2017) Antiulcer properties of fruits and vegetables: a mechanism based perspective. Food Chem Toxicol 108:104–119

    CAS  Article  Google Scholar 

  13. Hauck AK, Bernlohr DA (2016) Oxidative stress and lipotoxicity. J Lip Res 57:1976–1986

    CAS  Article  Google Scholar 

  14. Kempenich JW, Sirinek KR (2018) Acid peptic disease. Surg Clin N Am 98:933–944

    Article  Google Scholar 

  15. Kinoshita Y, Ishimura N, Ishihara S (2018) Advantages and disadvantages of long-term proton pump inhibitor use. J Neurogastroenterol Motil 24:182–196

    Article  Google Scholar 

  16. Lebda MA, El-Far AH, Noreldin AE, Elewa YHA, Jaouni SKA, Mousa SA (2018) Protective effects of Miswak (Salvadora persica) against experimentally induced gastric ulcers in rats. Oxid Med Cel Long.

    Article  Google Scholar 

  17. Lee IC, Baek HS, Kim SH, Moon C, Park SH, Kim SH, Shin IS, Park SC, Kim JC (2015) Effect of diallyl disulfide on acute gastric mucosal damage induced by alcohol in rats. Hum Exp Toxic 34:227–239

    CAS  Article  Google Scholar 

  18. Levine RL, Williams LA, Stadtman ER, Shacter E (1994) Carbonyl assays for determination of oxidatively modified proteins. Method Enzymol 233:346–357

    CAS  Article  Google Scholar 

  19. Lim JM, Song CH, Park SJ, Park DC, Cho HR, Jung GW, Bashir KMI, Kwang SK, Choi JS (2018) Protective effects of a triple-fermented barley extract (FBe) against HCl/EtOH-induced gastric mucosa damage in mice. Food Sci Nutr 6:2036–2046

    CAS  Article  Google Scholar 

  20. Livero FAR, da Silva LM, Ferreira DM, Galuppo LF, Borato DG, Prando TB, Lourenço EL, Strapasson RL, Stefanello MÉ, Werner MF, Acco A (2016a) Hydroethanolic extract of Baccharis trimera promotes gastroprotection and healing of acute and chronic gastric ulcers induced by ethanol and acetic acid. Naunyn-Schmiedeberg’s Arch Pharmacol. 389:985–998

    Article  Google Scholar 

  21. Lívero FA, Martins GG, Queiroz Telles JE, Beltrame OC, Petris Biscaia SM, Cavicchiolo Franco CR, Oude Elferink RP, Acco A (2016b) Hydroethanolic extract of Baccharis trimera ameliorates alcoholic fatty liver disease in mice. Chem Biol Interact. 260:22–32

    Article  Google Scholar 

  22. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  23. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474

    CAS  Article  Google Scholar 

  24. Nehra AK, Alexander JA, Loftus CG, Nehra V (2018) Proton pump inhibitors: review of emerging concerns. Mayo Clin Proc 93:240–246

    CAS  Article  Google Scholar 

  25. Pádua BC, Silva LD, Rossoni-Júnior JV, Humberto JL, Chaves MM, Silva ME, Pedrosa ML, Costa DC (2010) Antioxidant properties of Baccharis trimera in the neutrophils of Fisher rats. J Ethnopharmacol 129:381–386

    Article  Google Scholar 

  26. Rabelo ACS, Costa DC (2018) A review of biological and pharmacological activities of Baccharis trimera. Chem Biol Interact 296:65–75

    Article  Google Scholar 

  27. Rabelo ACS, de Araújo GR, Lúcio KP, Araújo CM, Miranda PHA, Silva BM, Carneiro ACA, Ribeiro EM, Lima WG, Souza GHB, Brandão GC, Costa DC (2017) Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. Rev Bras Farmacogn 27:729–738

    CAS  Article  Google Scholar 

  28. Rabelo ACS, Lúcio KP, Araújo CM, de Araújo GR, Miranda PHA, Carneiro ACA, Ribeiro EM, Silva BM, Lima WG, Costa DC (2018) Baccharis trimera protects against ethanol induced hepatotoxicity in vitro and in vivo. J Ethnopharmacol 215:1–13

    CAS  Article  Google Scholar 

  29. Repetto MG, Llesuy SF (2002) Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res 35:523–534

    CAS  Article  Google Scholar 

  30. Selmi S, Rtibi K, Grami D, Sebai H, Marzouki L (2017) Protective effects of orange (Citrus sinensis L.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat. Lip Heal Dis 16:152–164

    Article  Google Scholar 

  31. Stermer E (2002) Alcohol consumption and the gastrointestinal tract. IMAJ 4:200–203

    PubMed  Google Scholar 

  32. Wang L, Zhang H, Li C (2016) Omeprazole alleviates aristolochia manshuriensis kom-induced acute nephrotoxicity. PLoS ONE 11(10):e0164215. 7 Oct 2016)

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  33. Yang Y, Yin B, Lv L, Wang Z, He J, Chen Z, Wen X, Zhang Y, Sun W, Li Y, Zhao Y (2017) Gastroprotective effect of aucubin against ethanol-induced gastric mucosal injury in mice. Lif Sci 189:44–51

    CAS  Article  Google Scholar 

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This research was supported by Coordination of Improvement of Higher Level Personnel (CAPES) and Federal University of Ouro Preto (UFOP), Brazil.

Author information




ACSR carried out the experiment and wrote the article. FCC carried out oxidative stress dosages. MMB performed the part of histology. WGdL supervised the experimental part and assisted in the statistical analysis. DCC supervised the experimental part and assisted in writing the article.

Corresponding author

Correspondence to Ana Carolina Silveira Rabelo.

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Ethical statement

In the study we used animals approved by the Committee guidelines on Care and Use of Animal from Federal University of Ouro Preto, Brazil (No. 2016/01).

Conflict of interest

Ana Carolina Silveira Rabelo has no conflict of interest. Fernanda Caetano Camini has no conflict of interest. Melissa Maia Bittencourt has no conflict of interest. Kíssyla Lacerda has no conflict of interest. Wanderson Geraldo de Lima has no conflict of interest. Daniela Caldeira Costa has no conflict of interest.

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Rabelo, A.C.S., Camini, F.C., Bittencourt, M.M. et al. Baccharis trimera (carqueja) promotes gastroprotection on ethanol-induced acute gastric ulcer. ADV TRADIT MED (ADTM) (2020).

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  • Baccharis trimera
  • Carqueja
  • Gastroprotection
  • Ethanol
  • Gastric ulcer