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Influence of Moringa (Moringa oleifera) leaf extracts on the antioxidant and angiotensin-1 converting enzyme inhibitory properties of lisinopril

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Abstract

This study investigated the in vitro effect of various combinations of aqueous extracts of moringa (Moringa oleifera) leaves on the ACE inhibitory and antioxidant properties of lisinopril, which is a popular synthetic ACE inhibitor. Moringa leaves were air-dried and blended into powdery form. Thereafter, the aqueous extract was prepared and freeze-dried. The following sample mixtures were prepared: L = 100% lisinopril (1 mg/ml); M = 100% moringa extract (1 mg/ml); 1L + 3M = 25% lisinopril + 75% moringa extract; L + M = 50% lisinopril + 50% moringa extract; 3L + 1M = 75% lisinopril + 25% moringa extract. The results revealed that 100% moringa and the various combinations showed higher ACE inhibition than 100% lisinopril in both rat heart and lung homogenates. Furthermore, all the samples caused the inhibition of Fe2+-induced lipid peroxidation in rat heart and lung tissue homogenates, with moringa leaf extract having the highest inhibitory ability. All the samples also showed antioxidant properties (ferric reducing antioxidant property, 2,2’-azino-bis (-3-ethylbenzthiazoline-6-sulphonate [ABTS*] and 1,1-diphenyl-2-picrylhydrazyl [DPPH] free radicals scavenging abilities, and Fe2+ chelating ability). This study revealed that moringa leaf extracts could improve the antioxidant and ACE inhibitory properties of lisinopril. However, further in vivo experiments and clinical trials are recommended.

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Acknowledgements

The authors would like to thank Dr. S. A. Adefegha for his advice and encouragement during the drafting of the manuscript.

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Authors and Affiliations

  1. Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Nigeria

    Ganiyu Oboh, Ayokunle Olubode Ademosun, Oyewumi John Oyetomi & Taiwo Mary Adewuni

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  1. Ganiyu Oboh
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  2. Ayokunle Olubode Ademosun
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  3. Oyewumi John Oyetomi
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  4. Taiwo Mary Adewuni
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Correspondence to Ganiyu Oboh.

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The handling and use of the animals were in accordance with the National Institutes for Health Guide for the Care and Use of Laboratory Animals. The handling and use of the experimental animals was as approved by the Animal Ethics Committee of the School of Sciences, Federal University of Technology, Akure, Nigeria, with protocol reference SOS/14/02a.

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The authors declare no conflict of interests.

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Oboh, G., Ademosun, A.O., Oyetomi, O.J. et al. Influence of Moringa (Moringa oleifera) leaf extracts on the antioxidant and angiotensin-1 converting enzyme inhibitory properties of lisinopril. Orient Pharm Exp Med 18, 317–324 (2018). https://doi.org/10.1007/s13596-018-0317-y

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