In a downward transplantation experiment, warming stimulated growth and photosynthesis of Schima superba Gardn. et Champ., Syzygium rehderianum Merr. et Perry and Itea chinensis Hook. et Arn. via increased stomatal conductance. Warming had no effect on growth of Machilus breviflora (Benth.) Hemsl., indicating species-specific differences in response to warming.
Climate change has been shown to shift species composition and community structure in subtropical forests. Thus, understanding the species-specific responses of growth and physiological processes to warming is essential.
To investigate how climate warming affects growth, morphological and physiological performance of co-occurring tree species when they are growing at different altitudes.
Soils and 1-year-old seedlings of four subtropical co-occurring tree species (Schima superba Gardn. et Champ., Syzygium rehderianum Merr. et Perry, Itea chinensis Hook. et Arn. and Machilus breviflora (Benth.) Hemsl.) were transplanted to three altitudes (600 m, 300 m and 30 m a.s.l.), inducing an effective warming of 1.0 °C and 1.5 °C. Growth, morphological, and physiological performances of these seedlings were monitored.
When exposed to warmer conditions, aboveground growth of the four species except M. breviflora was strongly promoted, accompanied by increased light-saturated photosynthetic rate and stomatal conductance. Warming also significantly increased concentrations of non-structural carbohydrates in leaves of S. rehderianum and M. breviflora, stems of S. superba and S. rehderianum, and roots of I. chinensis. However, we did not detect any effect of warming on stomatal length and stomatal density.
Our results provide evidence that climate warming could have species-specific impacts on co-occurring tree species, which might subsequently shift species composition and forest structure.
Global mean temperatures are expected to rise continuously within this century (IPCC 2014). To survive in future climate warming scenarios, tree species must alter morphological and physiological characteristics for adaptation. Tree species which will not be able to adapt to high temperature may even be submitted to local extinction and/or range shifts (Thomas et al. 2004; Jump et al. 2006). These divergent effects of climate warming on co-occurring tree species may result in complex impacts on forest structure and composition (Peñuelas et al. 2007). Thus, understanding the changes in tree performance in response to climate warming is fundamental to predicting forest responses under future climate change.
Morphological (i.e., specific leaf area, stomatal traits) and physiological (i.e., photosynthesis) traits affecting tree growth may be key components of adaptive strategies for tree development under climate warming (Aspinwall et al. 2016; Trahan and Schubert 2016; Slot and Winter 2017; Rodgers et al. 2018; Wu et al. 2018). Photosynthesis, one of the most important physiological traits determining plant growth, often promoted by warming unless temperature exceeds the thermal threshold or results in soil water deficits (Wertin et al. 2011; Way et al. 2015). Previous studies investigated the effects of warming on photosynthesis through biochemical processes (Salvucci and Crafts-Brandner 2004) and diffusive processes (Prieto et al. 2009; Xu et al. 2016). Stomatal length and density, controlling the gas exchange between atmosphere and leaf tissues, are important morphological traits that can be affected by environmental factors such as soil water availability, light, and warming (Wu et al. 2018). In addition, changes in photosynthesis in response to warming may also be associated with shifts of non-structural carbohydrates (NSCs, soluble sugar, and starch) pools (Tang et al. 2016), which are products of photosynthesis and the major substrates for both primary and secondary plant metabolism. Starch is acting as a carbon reservoir for plant growth and respiration, and soluble sugars serve a critical osmotic function to maintain turgor and vascular transport (Dietze et al. 2014). However, results on NSCs responses of tree species to climate warming are variable. Studies have found that warming can decrease (Wang et al. 2012), increase (Djanaguiraman et al. 2011), or have little effect (Xu et al. 2012) on NSCs concentration in plants. Accordingly, investigating species-specific responses of NSCs in trees experiencing elevated temperature may improve our knowledge of how co-occurring tree species cope with climate warming (Hartmann and Trumbore 2016).
Patterns of tree growth in response to climate warming depend on many factors, including above-mentioned physiological and morphological processes, phenology, water and nutrient availability, functional type, and provenance. Several studies have found positive relationship between growth and temperature in tree species from high latitudes or altitudes (Drake et al. 2015; Anadon-Rosell et al. 2018). For example, Wang et al. (2012) found a significant stimulation of experimental warming on growth of Abies faxoniana in subalpine coniferous forests, which is characterized by low temperatures and a short growing season. However, other studies have indicated that, compared to either boreal or temperate species, tropical species may be near a high temperature threshold for photosynthesis that, if exceeded, will reduce CO2 assimilation and thereby growth (Way and Oren 2010). During the last decades, many inventories in subtropical and tropical regions highlighted that climate warming is modifying forest composition and structure (Sheldon et al. 2011; Zhou et al. 2013, 2014) and result in negative economic effects (Burke et al. 2015). Many of these changes should be consequences of divergent responses of co-occurring tree species to climate warming. Thus, examining whether the effects of warming on subtropical species are species-specific is an essential requirement to better understanding the future state of subtropical forests.
Existing thermal gradients provide a useful insight into modeling of species’ responses expected under future climate change (Kooyers et al. 2014). Transplantation experiments between high and low altitudes have been extensively used to test for the direct effects of temperature on plants and ecosystems (Walker et al. 2018). The altitudinal gradients of montane forests provide an opportunity to establish the relationships between tree species and multiple environmental factors (Salinas et al. 2011).
Zhou et al. (2011) demonstrated that the annual temperature in subtropical China has increased by approximately 1.0 °C in the past five decades. Hence, we conducted a downward transplantation experiment to investigate the effects of warming on tree growth and several functional traits. Saplings and soils from 600 m were transplanted to 300 m and 30 m to induce an effective warming of 1.0 °C and 1.5 °C. We selected Schima superba Gardn. et Champ., Syzygium rehderianum Merr. et Perry, Itea chinensis Hook. et Arn., and Machilus breviflora (Benth.) Hemsl., four naturally co-occurring tree species that inhabit subtropical montane forests in southern China. We addressed the following questions: (Anadon-Rosell et al. 2018) how does climatic warming affect growth of co-occurring tree species in the subtropical montane forest of southern China and (Aspinwall et al. 2016) what physiological and morphological processes are involved in the growth responses of these tree species to climate warming?
Materials and methods
Our study was conducted at the Dinghushan Biosphere Reserve, a UNESCO/MAB site located in central Guangdong Province in southern China (112°10′E, 23°10’N). This region has a typical subtropical monsoon climate, with annual precipitation of 1956 mm and annual mean air temperature of 21 °C (Zhou et al. 2014). The bedrock is sandstone and shale. Soils are classified as ultisols with a pH 4.0–4.9 in the top 5 cm (Zhou et al. 2011).
Transplantation and monitoring
We conducted a transplantation experiment in three field sites along an altitudinal gradient. A high-elevation site (600 m a.s.l) served as control to assess the effect of transplantation to a middle-elevation (300 m a.s.l) and a low-elevation site (30 m a.s.l). At each site, three blocks (3.0 m in length and width, 0.8 m in depth) were established in an open area and were exposed to natural sunlight and rainfall.
In April 2012, soil and 1-year-old seedlings were collected from a montane evergreen broadleaved forest close to the 600 m site. Three different layers of soils (0–20, 20–40, and 40–70 cm) were homogenized separately. Seedlings were stored in shade containers with soil from the collection forest. In May 2012, three different layers of soils were transferred into each block correspondingly. Seedlings were transplanted into each block in a randomized block design (n = 6 replicates per species). The four species included in this study were specifically selected due to their common occurrence and distribution range (existence in almost all regions along the altitudinal gradient) from the montane forest. They included Schima superba Gardn. et Champ., Syzygium rehderianum Merr. et Perry., Machilus breviflora (Benth.) Hemsl., and Itea chinensis Hook. et Arn.
From May 2013, air temperature, soil temperature, and volumetric soil water content at each block were continuously recorded and recently been published (Liu et al. 2017; Wu et al. 2019). The average air temperature at the altitude of 300 m and 30 m were 1.0 °C and 1.5 °C higher to the altitude of 600 m, respectively (Wu et al. 2019). The monthly mean volumetric soil water content was 0.18, 0.20, and 0.16 m3 m−3 at 600 m, 300 m, and 30 m, respectively (Wu et al. 2019).
D2H, leaf area, and photosynthesis measurements
In June 2014 and 2015, tree heights (H) and basal diameters (D) of each tree in each block were measured to calculate D2H. For specific leaf area (SLA), five fully expanded sun-exposed leaves were collected from three individuals per species in each block. Total areas of sampled leaves were determined by a portable leaf area meter (Li-3100A, Li-Cor, USA). The leaves were then oven dried for 72 h at 70 °C to determine dry weight. SLA was calculated as the ratio of leaf area to dry mass.
Leaf gas exchange was measured on newly developed and fully expanded leaves from three individuals per species in each block using a portable open path gas exchange system (Li-6400XT, Li-Cor, USA) supplying photosynthetic photon flux (PPFD) by red-blue light source (6400-02B). Light-saturated photosynthesis was measured at midday (between 0930 and 1300 h) on a monthly basis throughout the experiment, at PPFD of 1500 μmol m−2 s−1 and [CO2] of 400 μmol mol−1. Temperature and relative humidity in Licor-6400XT cuvettes were maintained at the initial air values throughout the measurements, representing the environmental conditions in the block during the day.
Non-structural carbohydrate (soluble sugar and starch)
To determine soluble sugar and starch concentration, one seedling per species from each block was randomly selected for destructive harvest on December 2014. Seedlings were separated into leaves, stem, and roots. Oven-dried plant organ samples were ground to fine powder in a ball mill. Soluble sugars were extracted using an ethanol technique and determined using the anthrone colorimetric assay (Ebell 1969). Starch was determined enzymatically using a total starch assay kit (Megazyme International Ireland Ltd., Wicklow, Ireland). Non-structural carbohydrate concentration was calculated as the sum of soluble sugar and starch concentrations.
Stomatal length and stomatal density
In June 2014, four leaves from three individuals per species were collected in each block. Leaf epidermises on the adaxial side were taken centrally in the leaf midway. The epidermises were mounted on a microscope slide and observed using a light microscope (DM2500, Leica, Germany). For each epidermal peel, 20 stomata were sampled to measure length and three fields (0.16 mm2) were sampled for density.
All analyses were conducted using SPSS 17.0 (SPSS Inc., Illinois, USA). We chose α equal to 0.05. Data normality and residual homogeneity were checked prior to analysis. Repeated-measures ANOVA were conducted to examine the main and interactive effects of transplantation, species, measuring (or sampling) time on light-saturated photosynthetic rate, stomatal conductance, D2H, and specific leaf area. In addition, the transplantation effect and its interaction with species on stomatal length, density, and NSCs were analyzed by two-way ANOVA. When the effects were significant, they were further analyzed using Tukey multiple comparison test (honestly significant difference). Relationships between light-saturated photosynthetic rate, leaf temperature, and stomatal conductance at each site were analyzed using non-linear and linear regression analysis, respectively.
D2H and specific leaf area
Transplantation and species significantly affected specific leaf area (SLA) and D2H (p < 0.01, Table 1). Transplantation from 600 to 300 m significantly increased D2H of I. chinensis, with no significant effect on other species. Transplantation from 600 to 30 m significantly increased D2H of S. superba, S. rehderianum and I. chinensis, with no significant effect on M. breviflora (Fig. 1).
Transplantation from 600 to 300 m significantly increased SLA of S. superba, M. breviflora, and I. chinensis in 2015 (p < 0.05, Fig. 1), but had no effect in 2014. No obvious difference was detected in SLA of all species between the altitudes of 30 and 600 m in 2014 and 2015.
Light-saturated photosynthetic rate of the four measured species in each site fluctuated along the experimental period. Interactive effects of transplantation and species on photosynthetic rate were detected (Table 1). Transplantation from 600 m (mean leaf temperature of 25.8 °C during measurements) to 300 m (mean leaf temperature of 27.2 °C during measurements) significantly increased photosynthetic rate of S. rehderianum (p < 0.01) and M. breviflora (p < 0.05) but had no effect on S. superba and I. chinensis (Fig. 2). Transplantation from 600 to 30 m (mean leaf temperature of 28.7 °C during measurements) significantly increased light-saturated photosynthetic rate and stomatal conductance of S. superba, S. rehderianum, M. breviflora and I. chinensis (p < 0.01 for all species, Fig. 2). Light-saturated photosynthetic rates were positively correlated with leaf temperatures and stomatal conductance for S. superba, S. rehderianum, M. breviflora, and I. chinensis (p < 0.05 for all species, Figs. 3 and 4). The regressions separated clearly between three sites for all four species.
Stomatal length and density
In general, transplantation had no significant effect on stomatal length and density (Table 1), with no interaction with species. There was no difference between the stomatal length and density of all tree species at the altitude of 300 m and 30 m. Transplantation only decreased the stomatal density of M. breviflora from 600 to 30 m (p < 0.05, Fig. 5).
Soluble sugar, starch, and non-structural carbohydrate content varied significantly between species and organs and were all affected significantly by transplantation (Tables 1 and 2). Transplantation significantly increased concentrations of non-structural carbohydrates in leaves of S. rehderianum and M. breviflora, stems of S. superba and S. rehderianum, and roots of I. chinensis (Table 3).
In this study, we found that temperatures of 1–1.5 °C above the control increased D2H in saplings of S. superba, S. rehderianum, and I. chinensis grown at warmer temperatures. These results did not support the widely assumed hypothesis that climate warming would have negative effect on subtropical tree growth (Clark et al. 2010). Several observational studies concerning canopy trees indicated negative correlations between growth and temperature in tropical forests (Clark et al. 2014). Some sapling studies suggest the opposite results (Cheesman and Klaus 2013; Fahey et al. 2016; Slot and Winter 2018). Consistent with positive effects on growth, we also found increased light-saturated photosynthetic rates for all four tree species grown in lower altitudes during both wet and dry seasons (Table 2; Fig. 2). Enhancements in photosynthetic rate in response to transplantation were largely associated with changes in stomatal conductance (Fig. 4). Previous studies found that stomatal conductance is negatively correlated with vapor pressure deficit (VPD), which often exhibit declines under warming because air relative humidity increases to a greater extent than temperature (Duursma et al. 2014). Therefore, in our study, increased temperature and lower VPD can lead to increased stomatal conductance and thus to photosynthetic rate.
It is clear from the relationships between light-saturated photosynthetic rates and seasonal temperature variation at prevailing temperatures that the optimum temperature for the saplings growing at lower altitudes seems to be higher than that at high altitude. In general, light-saturated photosynthetic rates had already commenced to decline at temperatures above 28–31 °C. This result indicated that climate warming will have negative impacts on photosynthesis of these saplings during the warm period of the year. In addition, photosynthesis exhibited at least partial thermal acclimation, which varied with species and altitudes, either from the seasonal variations (Fig. 2) or from leaf temperature-response curves (Fig. 3). This could be a predominant cause of the altitudinal shift in optimum temperature. Unfortunately, we cannot directly quantify the effects of acclimation on this result because we did not measure gas exchange at a set constant measurement leaf temperature (corresponding value were 25.8 °C, 27.2 °C, 28.7 °C in average for 600 m, 300 m, and 30 m, respectively, in this study). Clearly, more detailed temperature-response measurements in natural environments, which are quite limited, will be needed to focus on the capacity for thermal acclimation of photosynthesis and its consequences in tropical species.
Several morphological adjustments may have helped ensure plant acclimate to elevated temperature. When exposed to favorable environments (e.g., warm and wet), plants tend to produce higher specific leaf area (SLA) (Yuan et al. 2018), which is positively related to light-saturated photosynthetic rate and growth rate (Perez-Harguindeguy et al. 2013). In addition, there is a clear difference in the response of boreal and tropical species, with the tropical species changing SLA more for a given change in temperature (Poorter et al. 2009). In our study, SLA showed substantial phenotypic plasticity after 3 years, as indicated by a significant increase in SLA of S. superba and I. chinensis with transplantation from 600 to 300 m, in accordance with previous studies (Hamann et al. 2018; Barbosa et al. 2018). The increase in SLA with downward transplantation can be achieved through decreases in leaf density and/or leaf thickness, leading to greater efficiency in light capture (Gratani 2014). In addition to altered SLA, higher photosynthetic rate under warming might be associated with warming-induced changes in stomatal density and length, which are the two primary morphological regulators controlling the response of stomatal conductance to climatic stresses (Franks et al. 2015). Some earlier studies have shown that stomata traits of trees can vary with changing temperature (Wu et al. 2018). However, in our study, only transplantation from 600 to 30 m decreased the stomatal density of M. breviflora (Fig. 5). In general, lower stomatal density would prevent excessive plant water use, as a potential tool for enhancing drought tolerance without significantly affecting photosynthetic capacity (Franks et al. 2015; Hepworth et al. 2015).
Non-structural carbohydrates (NSCs, sum of soluble sugar and starch) stores play a functional role for plant metabolism under stress (Dietze et al. 2014). Several studies found that warming may increase (Djanaguiraman et al. 2011; Zheng et al. 2018), decrease (Tang et al. 2016), or have little effect (Marias et al. 2016) on the concentration of NSCs in plants. In the present study, warming induced an increase of NSCs in leaves of S. rehderianum and M. breviflora, stems of S. superba and S. rehderianum, roots of I. chinensis, and concurrent with a decrease of NSCs in roots of M. breviflora. Our results also indicated an increase in soluble sugar and a decrease in starch. This may indicate new production of soluble sugar with concurrent consumption of starch or the hydrolyzation of starch to soluble sugar in response to warming (Kozlowski 1992), which allows the maintenance of water absorption and cell turgor under water deficits. Marias et al. (2016) also found that warming induced significant increase of soluble sugars accompanied by a reduction of starch in leaves of Pinus ponderosa and Pseudotsuga menziesii.
In this study, the distinct responses of growth and functional traits to elevated temperature among the four tree species revealed different adaptive strategies under climate warming. S. superba is a representative dominant species in subtropical and tropical forest in China, valued commercially for its timber and can also prevent the spread of fire. In this study, it had the highest photosynthetic rate of all species, regardless of transplantation, and also showed greatest enhancement in photosynthetic rate and growth with warming of all species. M. breviflora, which is a shade-tolerant species that distributed in southern subtropical and tropical forests, was the only species showing no change in growth with transplantation. In this study, it is apparent that elevated temperatures can markedly stimulate growth of S. superba, S. rehderianum, and I. chinensis at the sapling stage. Currently, we are not sure the degree to which our results, obtained from saplings of four species, can be extended to mature trees. Gunderson et al. (2010) made direct comparisons of acclimation potential for seedling-sapling size trees and those of mature trees under experimental warming. They found that photosynthesis acclimation occurred regardless of tree age and size. If our results can similarly translate to mature trees, this could result in shifts of species composition and community structure in subtropical forests, with long-term climatic consequences. Ideally, further experiments using mature trees from a wide variety of species, which is expensive and challenging, would need to be carried out to ascertain the response of mature trees to climate warming. The results from younger trees, as in this study, offer a valuable insight into the potential effects on a wider range of tree species.
We investigated how transplantation-induced warming affected morphological and physiological performance of four subtropical co-occurring tree species. Although relatively high temperature already exist in subtropical areas, we found positive effect on growth of S. superba, S. rehderianum, and I. chinensis, accompanied by increased photosynthetic rate and specific leaf area. Stimulations of photosynthesis in response to warming conditions were associated with those in leaf temperature and stomatal conductance. Warming also significantly increased concentrations of NSCs in leaves of S. rehderianum and M. breviflora, stems of S. superba and S. rehderianum, and roots of I. chinensis. Our results highlight that subtropical tree species have species-specific capacities to acclimate to elevated temperature that will likely occur in the coming decades under climate change, and ultimately improve our understanding of performances in the adaptive potential of tropical saplings. Results from our sapling study may not be directly extrapolated to adult studies. However, our study offers a valuable insight into the potential effects on a wider range of tree species. Further studies need to be conducted on mature trees to obtain more information in tropical forest responses to future climates.
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We thank the editor and the two anonymous reviewers for valuable comments.
Data availability statement
The datasets generated during and/or analyzed during the current study are not publicly available because the authors are working on another manuscript using the same dataset but are available from the corresponding author on reasonable request.
This study was jointly funded by the National Natural Science Foundation of China (31800511, 31570482 and 31670453), the Science and Technology Innovation Project of Guangdong Province Forestry (Grant No. 2019KJCX023), and the Fund of Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, Chinese Academy of Sciences (DE2018028).
Statement on ethical approval
We declare that this study have obtained the appropriate permissions from the responsible authorities of “Dinghushan Biosphere Reserve.”
Conflict of interest
The authors declare that they have no conflict of interest.
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Contribution of co-authors JL and GZ conceived this study; YL, YX, TW, SL, and JW conducted the experiment; YL analyzed the results. All the authors contributed to writing and editing the manuscript.
Handling Editor: Marcus Schaub
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Li, Y., Xu, Y., Li, Y. et al. Warming effects on morphological and physiological performances of four subtropical montane tree species. Annals of Forest Science 77, 2 (2020). https://doi.org/10.1007/s13595-019-0910-3
- Stomatal traits
- Non-structural carbohydrates