The role of extractives in the natural durability of the heartwood of Dicorynia guianensis Amsh: new insights in antioxydant and antifungal properties

  • Jean-Baptiste Say Anouhe
  • Florence Bobelé Niamké
  • Milcard Faustin
  • David Virieux
  • Jean-Luc Pirat
  • Augustin Amissa Adima
  • Seraphin Kati-Coulibaly
  • Nadine Amusant
Original Paper

Abstract

Key message

The natural durability of Dicorynia guianensis Amsh ’s Heartwood is conferred by the high content of antioxidant phenolic compounds, especially tannins and flavonoids combined with the presence of fungistatic alkaloids. The content of phenolic compounds increases according to the natural durability classes, from durable wood (class 2) to moderately durable wood (class 3) and correlated to the antioxidant capacity.

Context

The heartwood of Dicorynia guianensis Amsh is resistant to white rot fungi decay, but the mechanism of this natural durability is not fully elucidated.

Aims

Biochemical studies were carried out in order to better understand the role of extractives in natural durability of D. guianensis.

Methods

The powders from durable and moderately durable heartwood were extracted with methanol, ethanol, and hot water. The quantity of total phenols, tannins, and flavonoids as well as antioxidant activity, evaluated by 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) were determined using colorimetric methods. Antifungal activity was assessed by using two white rot fungi. The bioactive fractions and compounds were obtained using bio-guided fractionation, HPLC isolation, MS and RMN spectroscopic analyses.

Results

Durable woods contain higher amounts of heartwood extract and antioxidant activity. Antioxidant activity was highly correlated with the content of phenolics. The purification of the most antioxidant fraction FII affords the characterization of (+)-catechin (−)-epicatechin, neoastilbin, astilbin, and isoastilbin. Alkaloid fraction FIII exhibits dose-dependent fungistatic activity against Pycnoporus sanguineus Linnaeus and Trametes versicolor Quelet.

Conclusion

Phenolic antioxidants and fungistatic alkaloids positively impact the natural durability of D. guianensis.

Keywords

Dicorynia guianensis Heartwood Natural durability Antifungal Antioxidant Phenols Alkaloid 

Notes

Acknowledgements

This work was financially supported by the Cooperation and Cultural Action (SCAC) of the Embassy of France in Côte d’Ivoire, CIRAD and French National Research Agency (CEBA, ref. ANR-10-LABX-0025) as part of the “Investissement d’Avenir” program.

Co-author contributions

J.B Anouhe assured the conception and realization of all chemical and antioxidant experiments. He wrote the first draft of the paper. Nadine Amusant supervised the experimental work and coordinated the research project. Florence Niamké coordinated the antifungal activity tests and helped in writing and editing the manuscript. David Virieux and Jean-Luc Pirat assured the training of J-B Anouhe on spectroscopic measurements. Milcard Faustin, Amissa Adima, and Seraphin Kati-coulibaly were involved in the critical reading of the results. The final correction of the paper was read and approved by all authors.

Supplementary material

13595_2018_691_MOESM1_ESM.pdf (1.2 mb)
ESM 1 (PDF 1216 kb)

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Copyright information

© INRA and Springer-Verlag France SAS, part of Springer Nature 2018

Authors and Affiliations

  • Jean-Baptiste Say Anouhe
    • 1
  • Florence Bobelé Niamké
    • 2
  • Milcard Faustin
    • 3
  • David Virieux
    • 4
  • Jean-Luc Pirat
    • 4
  • Augustin Amissa Adima
    • 2
  • Seraphin Kati-Coulibaly
    • 1
  • Nadine Amusant
    • 3
  1. 1.Laboratoire de Nutrition et PharmacologieUFR Biosciences, Université Felix Houphouët Boigny de Cocody-AbidjanAbidjan 22Côte d’Ivoire
  2. 2.LAPISEN, Groupe de recherche en Chimie des Eaux et des Substances NaturellesInstitut National Polytechnique Houphouët BoignyYamoussoukroCôte d’Ivoire
  3. 3.CIRAD, UMR EcoFoG, AgroParisTech, CNRS, INRA, CIRADUniversité des Antilles, Université de GuyaneCayenneFrance
  4. 4.AM2N, Institut Charles GerhardtUMR 5253MontpellierFrance

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