A scientific note on improved isolation methods for Melissococcus plutonius from diseased Apis mellifera larvae
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European foulbrood (EFB) caused by Melissococcus plutonius is a significant infectious disease of honey bee brood. EFB is typically diagnosed based on the visual inspection of brood combs and detection of diseased larvae; however, since symptoms of EFB may easily be confused with other diseases or abnormalities in larvae, verification of the presence of M. plutonius in diseased larvae is required for a reliable diagnosis of EFB in many cases. The isolation and storage of M. plutonius strains are also important for future EFB studies. However, difficulties are sometimes associated with the isolation of M. plutonius from diseased larvae by culture methods due to the growth of secondary bacteria associated with EFB.
In an inspection of foulbrood on May 16, 2017, an abnormal Apis melliferacolony was detected in an apiary in Saitama prefecture, Japan. The colony showed irregular capping, and dead larvae were observed in uncapped cells. Gram-positive cocci were detected in dead larvae by...
KeywordsEuropean foulbrood Melissococcus plutonius Apis mellifera Enterococcus faecalis royal jelly
YN and MI: Diagnosed the EFB case described in this study and performed experiments; RA: Designed the first experiment; DT: Designed the second experiment and contributed to writing the manuscript. All authors read and approved the final manuscript.
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Conflict of interest
The authors declare that they have no conflict of interest.
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