Horticulture, Environment, and Biotechnology

, Volume 59, Issue 2, pp 285–292 | Cite as

Expression, glycosylation, and function of an anti-rabies virus monoclonal antibody in tobacco and Arabidopsis plants

  • Ilchan Song
  • Sol-Ah Park
  • Dalmuri Han
  • Hae Kyung Lee
  • Hyun Joo An
  • Kisung Ko
Research Report Tissue Culture/Biotechnology
  • 40 Downloads

Abstract

Plants have emerged as one of the most attractive systems for producing human therapeutic proteins against viral diseases. These include diagnostic reagents, vaccines, and antibodies. This process is known as molecular biofarming. The objective of this study was to develop and evaluate tobacco and Arabidopsis as plant platforms for producing human anti-rabies monoclonal antibody (mAb). Both tobacco and Arabidopsis transgenic plants were generated by Agrobacterium-mediated transformation. Purification of mAb SO57K from each plant was performed with ammonium sulfate-mediated precipitation and protein A affinity columns. SDS–PAGE analysis showed that the purity of mAb SO57K obtained from each transgenic plant was similar, whereas Arabidopsis showed approximately twofold greater protein expression than tobacco. The N-glycosylation was not significantly different between proteins from the two plant species, with both showing oligo-mannose glycan structures. The mAbs SO57 derived from both the model plants had similar neutralizing efficacy against target virus strain CVS-11. Taken together, tobacco and Arabidopsis are both promising platforms for producing a human anti-rabies mAb.

Keywords

Molecular biofarming Platform Tobacco Arabidopsis Transgenic plant Anti-rabies monoclonal antibody Neutralizing efficacy 

Notes

Acknowledgements

This research was supported by the Chung-Ang University Research Scholarship Grants in 2015 and the Korean Rural Administration (Grant Code # PJ011110).

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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ilchan Song
    • 1
  • Sol-Ah Park
    • 1
  • Dalmuri Han
    • 2
  • Hae Kyung Lee
    • 2
  • Hyun Joo An
    • 3
  • Kisung Ko
    • 1
  1. 1.Department of Medicine, College of MedicineChung-Ang UniversitySeoulKorea
  2. 2.Division of Bacterial Disease ResearchKorea Centers for Disease Control and PreventionOsongKorea
  3. 3.Graduate School of Analytical Science and TechnologyChungnam National UniversityDaejeonKorea

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