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Horticulture, Environment, and Biotechnology

, Volume 59, Issue 2, pp 231–238 | Cite as

Development of Bi gene-based SNP markers for genotyping for bitter-free cucumber lines

  • Jelli Venkatesh
  • Kihwan Song
  • Joung-Ho Lee
  • Jin-Kyung Kwon
  • Byoung-Cheorl Kang
Research Report Genetics and Breeding
  • 129 Downloads

Abstract

High contents of cucurbitacin compounds result in a bitter taste in cucumbers. The Bi locus mapped to chromosome 6 is responsible for cucumber bitterness. The Bi gene encodes oxidosqualene cyclase, a key enzyme in the cucurbitacin biosynthetic pathway. Two alleles of the Bi gene responsible for the phenotypic variation in bitterness differ by a single point mutation in the coding region. An efficient genotyping system is necessary to allow breeders to screen for bitterness alleles in their breeding programs. In the present study, the single nucleotide polymorphism located within the Bi gene, which is directly linked to cucumber bitterness, was utilized for gene-based marker development. We developed reliable and cost-effective high-resolution melting (HRM)- and Kompetitive Allele-Specific PCR (KASP)-based molecular markers (BiHRM1 and Bi-KASP). These gene-based markers should enhance the accuracy and effectiveness of marker-assisted selection for bitter-free cucumber lines in cucumber breeding programs.

Keywords

The Bi locus Bitterness Cucumber HRM KASP Marker-assisted selection 

Notes

Acknowledgements

This work was supported by a grant from the Bio-industry Technology Development Program (115079-2) of iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry), and a Grant (710001-07) from the Vegetable Breeding Research Center through the Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs.

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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jelli Venkatesh
    • 1
  • Kihwan Song
    • 2
  • Joung-Ho Lee
    • 1
  • Jin-Kyung Kwon
    • 1
  • Byoung-Cheorl Kang
    • 1
  1. 1.Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life SciencesSeoul National UniversitySeoulKorea
  2. 2.Department of Bioresources EngineeringSejong UniversitySeoulKorea

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