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Horticulture, Environment, and Biotechnology

, Volume 59, Issue 2, pp 189–198 | Cite as

Auxin positively regulates nitrogen remobilization in cucumber leaves

  • Yalin Du
  • Lianxue Fan
  • Chunyu Tian
  • Tao Wu
Research Report Genetics and Breeding
  • 90 Downloads

Abstract

Nitrogen (N) remobilization is an important N metabolic process in plants. The objective of this study was to increase our understanding of the relationship between low N tolerance and N remobilization in cucumber. Two cucumber cultivars, namely D0328 and D0422, were included in the analyses, the former of which displays a stronger capacity for low N tolerance. Source leaf transcriptomes were analyzed, which revealed differentially expressed genes in a number of interesting pathways, including amino acid biosynthesis, tyrosine and tryptophan biosynthesis, plant hormone signal transduction, and E3 ligase activity, implying that these pathways are involved in low N tolerance in cucumber. In agreement with transcriptome data, old D0328 leaves had significantly higher tryptophan and indole-3-acetic acid contents than old D0422 leaves under low N conditions. N remobilization efficiency in old leaves was increased by naphthaleneacetic acid treatment, whereas it was repressed by antiauxins α-(p-chlorophenoxy) isobutyric acid treatment. Taken together, the results of this study demonstrate that auxin promotes N remobilization in cucumber leaves during the reproductive stage of plant development and this positive regulation is associated with low N tolerance in cucumber.

Keywords

Auxin Cucumber Nitrogen Remobilization Tolerance Tryptophan 

Notes

Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 31101545); Program for New Century Excellent Talents in Heilongjiang Provincial University (No. 1452G03); University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (No. UNPYSCT-2015001); ‘Academic backbone’ Project of Northeast Agricultural University (No. 16XG05); ‘Young Talents’ Project of Northeast Agricultural University (No. 14QC07); Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture/Northeast Agricultural University (No. neauhc201601).

Supplementary material

13580_2018_20_MOESM1_ESM.doc (42 kb)
Supplementary material 1 (DOC 42 kb)
13580_2018_20_MOESM2_ESM.xls (214 kb)
Supplementary material 2 (XLS 213 kb)
13580_2018_20_MOESM3_ESM.xls (144 kb)
Supplementary material 3 (XLS 144 kb)

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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yalin Du
    • 1
    • 2
  • Lianxue Fan
    • 1
    • 2
  • Chunyu Tian
    • 1
    • 2
  • Tao Wu
    • 1
    • 2
  1. 1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of AgricultureNortheast Agricultural UniversityHarbinChina
  2. 2.Horticulture CollegeNortheast Agricultural UniversityHarbinChina

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