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Transcriptomic and gene expression changes in response to postharvest surface pitting in ‘Lingwu Long’ jujube fruit

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Abstract

‘Lingwu Long’ jujube (Ziziphus jujuba cv. Mill) is a fresh fruit variety that is popular in China. Low temperature storage is an important method for retaining fruit quality postharvest. However, jujube fruits are susceptible to surface pitting under low temperature storage. Therefore, we performed transcriptome analysis to investigate the changes in gene expression in surface-pitted jujube fruit (SPF). First, we developed cDNA libraries of SPF and surface-intact fruit (SIF) that had been stored at − 1 ± 0.5 °C. As a result, 21,136,649 and 21,663,126 high-quality clean reads were generated and 47,386 contigs were obtained. Second, we performed sequences annotation by gene description and gene ontology (GO term) analysis, which clustered the genes into three functional groups. A total of 2706 predicted proteins were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes database. An analysis of differential gene expression between SPF and SIF revealed more than 1210 genes that are differentially expressed in jujube in response to surface-pitting under low temperature storage, which are involved in lipid, carbohydrate, energy, amino acid, terpenoids, and polyketides metabolism, as well as membrane transport, signal transduction, translation, signal transduction, and biosynthesis of other secondary metabolites. We investigated the pattern of phenylalanine ammonia lyase gene expression in SPF by quantitative reverse-transcription PCR analysis. Our results provide new insights into the biology of surface pitting in jujube, which occurs under low temperature storage. Our findings help confirm the low-temperature tolerance pathways that function in postharvest fruit.

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Acknowledgements

This work was supported by the Key Projects in the National Science and Technology Pillar Program during the Twelve Five-year Plan Period of China (2015BAD16B02) and Natural Science Foundation of Tianjin (17JCZDJC34300).

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Author notes

  1. Xia Liu and Tengyue Wang have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, China

    Xia Liu, Tengyue Wang, Limei Li & Xihong Li

  2. Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, China

    Xia Liu & Yong Wang

  3. Tianjin Gasin-Donghui Preservation Technologies Co., Ltd., Tianjin, China

    Xia Liu & Lan Chen

  4. Sichuan Province Key Laboratory of Grain and Oil Processing and Food Safety, Food and Bioengineering College, Xihua University, Chengdu, China

    Yage Xing

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  1. Xia Liu
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  2. Tengyue Wang
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  3. Lan Chen
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  4. Limei Li
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Correspondence to Xihong Li.

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Liu, X., Wang, T., Chen, L. et al. Transcriptomic and gene expression changes in response to postharvest surface pitting in ‘Lingwu Long’ jujube fruit. Hortic. Environ. Biotechnol. 59, 59–70 (2018). https://doi.org/10.1007/s13580-018-0007-1

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