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Protective enzymes and genes related to the JA pathway are involved in the response to root-knot nematodes at high soil temperatures in tomatoes carrying Mi-1

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  • Tissue Culture/Biotechnology
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Abstract

Root-knot nematodes (RKNs; Meloidogyne spp.) are obligate endoparasites that infect a large number of crop plants and cause severe yield losses. Tomato cultivars carrying the Mi-1 gene conferring root-knot nematode resistance have been widely used, but this gene loses its effectiveness at soil temperatures above 28°C. In this study, the mechanism of the loss of resistance to RKNs at high soil temperatures was examined using LA0655 (Solanum lycopersicum cv. Anahu), which contains the Mi-1 gene. It was found that high soil temperatures delayed the expression of the Mi-1 gene and reduced the activities of superoxide dismutase, peroxidase, chitinase, and β-1,3-glucanase. Although genes in the jasmonic acid (JA) pathway exhibited an obvious response at high soil temperatures, this response could not prevent the invasion of RKNs; indeed, at 30 days after inoculation with RKNs, the plants produced large numbers of root knots and egg masses at a soil temperature of 32°C.

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Authors and Affiliations

  1. College of Plant Science and Technology, Beijing University of Agriculture, No. 7 BeiNongRoad, Changping District, Beijing, 102206, P. R. China

    Canli Hu, Wenchao Zhao, Jingwei Fan, Zilong Li & Shaohui Wang

  2. Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, No. 7 BeiNongRoad, Changping District, Beijing, 102206, P. R. China

    Rui Yang & JianLi Wang

  3. Biological Science and Technology College, Beijing University of Agriculture, No. 7 BeiNongRoad, Changping District, Beijing, 102206, P. R. China

    Fukuan Zhao

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  1. Canli Hu
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  2. Wenchao Zhao
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  3. Jingwei Fan
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  4. Zilong Li
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  5. Rui Yang
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  6. Fukuan Zhao
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  7. JianLi Wang
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  8. Shaohui Wang
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Correspondence to Shaohui Wang.

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These authors contributed equally to this work.

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Hu, C., Zhao, W., Fan, J. et al. Protective enzymes and genes related to the JA pathway are involved in the response to root-knot nematodes at high soil temperatures in tomatoes carrying Mi-1 . Hortic. Environ. Biotechnol. 56, 546–554 (2015). https://doi.org/10.1007/s13580-015-0146-6

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  • DOI: https://doi.org/10.1007/s13580-015-0146-6

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