Classical differentiation protocols upregulate the expression of the axon guidance genes PLXNA2 and SEMA3C in SH-SY5Y neuroblastoma cells
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The neuroblastoma-derived SH-SY5Y cell line is extensively used in Parkinson’s disease molecular studies for evaluating neurotoxicity or testing neuroprotective effects of drugs . Usually, differentiation is induced, before treatments, by exposure to diverse compounds, most commonly all-trans retinoic acid (atRA) or phorbol 12-myristate, 13-acetate (PMA), for 7 days at least . Both atRA and PMA treatments entail the emission of cytoplasmic axon-like processes and changes in enzymatic activity and gene expression, toward that typical of adult neurons [3, 4]. In developing nervous system, class A plexins associate with neuropilins acting as eterodimeric receptors for the secreted class 3 semaphorins . This plexins/semaphorins system is involved in axon navigation into the depths of tissues with diverse members of the class 3 semaphorins acting with repulsive or attractive effects. Here, we evaluated the expression of mRNA and protein levels of the PLXNA2 and SEMA3C...
KeywordsPlexinA2 Semaphorin3C Parkinson’s disease Neuronal differentiation SH-SY5Y
Tris buffered saline plus 0.1% Tween 20
PBS + 0.1% Tween 20
Ribosomal Protein S9
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis
Fetal bovine serum
Compliance with ethical standards
Conflict of interest
All authors declare they have no affiliations with or involvement in any organization with any financial or non-financial interest.
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