Human Cell

, Volume 32, Issue 3, pp 397–400 | Cite as

Classical differentiation protocols upregulate the expression of the axon guidance genes PLXNA2 and SEMA3C in SH-SY5Y neuroblastoma cells

  • Ivan PrestaEmail author
  • Annalidia Donato
  • Natalia Malara
  • Emilio Russo
  • Marta Letizia Hribal
  • Giuseppe Donato
Letter to the Editor

Dear Editor,

The neuroblastoma-derived SH-SY5Y cell line is extensively used in Parkinson’s disease molecular studies for evaluating neurotoxicity or testing neuroprotective effects of drugs [1]. Usually, differentiation is induced, before treatments, by exposure to diverse compounds, most commonly all-trans retinoic acid (atRA) or phorbol 12-myristate, 13-acetate (PMA), for 7 days at least [2]. Both atRA and PMA treatments entail the emission of cytoplasmic axon-like processes and changes in enzymatic activity and gene expression, toward that typical of adult neurons [3, 4]. In developing nervous system, class A plexins associate with neuropilins acting as eterodimeric receptors for the secreted class 3 semaphorins [5]. This plexins/semaphorins system is involved in axon navigation into the depths of tissues with diverse members of the class 3 semaphorins acting with repulsive or attractive effects. Here, we evaluated the expression of mRNA and protein levels of the PLXNA2 and SEMA3C...


PlexinA2 Semaphorin3C Parkinson’s disease Neuronal differentiation SH-SY5Y 



Horseradish peroxidase


Tris buffered saline plus 0.1% Tween 20


Phosphate-buffered saline


PBS + 0.1% Tween 20


Ribosomal Protein S9


Sodium dodecyl sulphate-polyacrylamide gel electrophoresis


Fetal bovine serum


Compliance with ethical standards

Conflict of interest

All authors declare they have no affiliations with or involvement in any organization with any financial or non-financial interest.

Supplementary material

13577_2019_246_MOESM1_ESM.docx (997 kb)
Supplementary material 1 (DOCX 997 KB)


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Health SciencesUniversity “Magna Graecia” of Catanzaro, V.le EuropaCatanzaroItaly
  2. 2.Department of Medical and Surgical SciencesUniversity “Magna Graecia” of CatanzaroCatanzaroItaly
  3. 3.Department of Clinical and Experimental MedicineUniversity “Magna Graecia” of CatanzaroCatanzaroItaly

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