MiR-1179 inhibits the proliferation of gastric cancer cells by targeting HMGB1
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Gastric cancer is one of the most common aggressive malignancies with high incidence and mortality. Increasing evidence has suggested that microRNAs (miRNAs) are involved in the initiation and development of gastric cancer. Here, we found that miR-1179 was significantly down-regulated in both gastric cancer tissues and cell lines. Decreased expression of miR-1179 was remarkably correlated with the increased tumor size, higher tumor stage and lymph node metastasis of gastric cancer patients. Overexpression of miR-1179 significantly inhibited the proliferation and invasion of gastric cancer cells. Further molecular experiments showed that miR-1179 bound the 3′-untranslated region of the high mobility group box 1 (HMGB1) and decreased the expression of HMGB1 in gastric cancer cells. The level of HMGB1 was negatively correlated with the expression of miR-1179 in gastric cancer tissues. Rescue experiment demonstrated that restore the expression of HMGB1 significantly inversed the inhibitory effect of miR-1179 on the proliferation of gastric cancer cells. Our results uncovered the novel function of miR-1179/HMGB1 axis in the progression of gastric cancer.
KeywordsGastric cancer MiR-1179 HMGB1
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Conflict of interest
The authors declare that they have no conflicts of interests.
- 7.Fabian MR, Sonenberg N, Filipowicz W. Regulation of mRNA translation and stability by microRNAs. Annu Rev Biochem. 2010;79:351–79. https://doi.org/10.1146/annurev-biochem-060308-103103.Google Scholar
- 8.Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116(2):281–97.Google Scholar
- 13.Qu H, Xu W, Huang Y, Yang S. Circulating miRNAs: promising biomarkers of human cancer. Asian Pac J Cancer Prev APJCP. 2011;12(5):1117–25.Google Scholar
- 20.Song L, Dai Z, Zhang S, Zhang H, Liu C, Ma X, et al. MicroRNA-1179 suppresses cell growth and invasion by targeting sperm-associated antigen 5-mediated Akt signaling in human non-small cell lung cancer. Biochem Biophys Res Commun. 2018;504(1):164–70. https://doi.org/10.1016/j.bbrc.2018.08.149.Google Scholar
- 22.Xu X, Cai N, Zhi T, Bao Z, Wang D, Liu Y, et al. MicroRNA-1179 inhibits glioblastoma cell proliferation and cell cycle progression via directly targeting E2F transcription factor 5. Am J Cancer Res. 2017;7(8):1680–92.Google Scholar
- 24.Huang CY, Chiang SF, Ke TW, Chen TW, Lan YC, You YS, et al. Cytosolic high-mobility group box protein 1 (HMGB1) and/or PD-1 + TILs in the tumor microenvironment may be contributing prognostic biomarkers for patients with locally advanced rectal cancer who have undergone neoadjuvant chemoradiotherapy. Cancer Immunol Immunother. 2018;67(4):551–62. https://doi.org/10.1007/s00262-017-2109-5.Google Scholar
- 31.Feng XJ, Liu SX, Wu C, Kang PP, Liu QJ, Hao J, et al. The PTEN/PI3K/Akt signaling pathway mediates HMGB1-induced cell proliferation by regulating the NF-kappaB/cyclin D1 pathway in mouse mesangial cells. Am J Physiol Cell Physiol. 2014;306(12):C1119-28. https://doi.org/10.1152/ajpcell.00385.2013.Google Scholar