The effect of human sperm chromatin maturity on ICSI outcomes
- 155 Downloads
Because sperm chromatin may play a key role in reproductive success, we verify the associations between sperm chromatin abnormalities, embryo development and the ability to achieve pregnancy. The evaluation of sperm chromatin maturity using aniline blue (AB), chromomycin A3 (CMA3) and toluidine blue (TB) staining were carried out in group of males from infertile couples that underwent ICSI. Low levels of sperm chromatin abnormalities (< 16%) were found in most subjects (> 50%). A higher percentage of TB-positive sperm cells were discovered in the men from couples who achieved ≤ 50% fertilized oocytes compared to men who achieved > 50%. No significant differences were discovered by the applied tests between the men from couples who achieved ≤ 50% and those who achieved > 50% high-quality embryos on the 3rd or 5th day after fertilization, nor between the men from couples who achieved pregnancy and those who failed. The sperm chromatin maturity did not correlate with the ICSI results. However, the ROC analysis revealed a significant predictive value of TB-positive spermatozoa only for fertilization. Therefore, the TB assay can be considered as a useful test for the prediction of fertilization. Our findings suggest that the level of sperm chromatin abnormalities of the examined men was not clinically significant. No found associations between sperm chromatin maturity and embryo development and the ability to achieve pregnancy. We could not exclude the effects of the repairing processes in the fertilized oocyte. The use of complementary tests that verify the status of the sperm chromatin seems justified.
KeywordsSperm Chromatin In vitro fertilization (IVF) Male infertility
This study was supported by the Pomeranian Medical University, Szczecin, Poland Grants No. WNoZ-322-04/S/2016 and No. FSN-322-5/2016.
Compliance with ethical standards
Conflict of interest
The authors declare there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
- 4.Tahmasbpour E, Balasubramanian D, Agarwal A. A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART). J Assist Reprod Genet. 2014;31:1115–37. https://doi.org/10.1007/s10815-014-0280-6.CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Wdowiak A, Wdowiak A, Bakalczuk S, Bakalczuk G. Relationship between alcohol consumption and a sperm nuclear DNA fragmentation and pregnancy. Postep Androl Online. 2016;3:14–21.Google Scholar
- 7.Fernandez-Encinas A, García-Peiró A, Ribas-Maynou J, et al. Characterization of nuclease activity in human seminal plasma and its relationship to semen parameters, sperm DNA fragmentation and male infertility. J Urol. 2016;195:213–9. https://doi.org/10.1016/j.juro.2015.07.089.CrossRefPubMedGoogle Scholar
- 9.Hotaling JM, Smith JF, Rosen M, Muller CH, Walsh TJ. The relationship between isolated teratozoospermia and clinical pregnancy after in vitro fertilization with or without intracytoplasmic sperm injection: a systematic review and meta-analysis. Fertil Steril. 2011;95:1141–5. https://doi.org/10.1016/j.fertnstert.2010.09.029.CrossRefPubMedGoogle Scholar
- 13.van der Horst G, du Plessis S. Not just the marriage of Figaro: but the marriage of WHO/ESHRE semen analysis criteria with sperm functionality. Postep Androl Online. 2017;4(1):6–21.Google Scholar
- 26.Kazerooni T, Asadi N, Jadid L, et al. Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J Assist Reprod Genet. 2009;26:591–6. https://doi.org/10.1007/s10815-009-9361-3.CrossRefPubMedPubMedCentralGoogle Scholar
- 38.World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th ed. Geneva: World Health Organization Press; 2010.Google Scholar
- 53.Piasecka M, Gaczarzewicz D, Laszczyńska M, Starczewski A, Brodowska A. Flow cytometry application in the assessment of sperm DNA integrity of men with asthenozoospermia. Folia Histochem Cytobiol. 2007;45(1):127–36.Google Scholar
- 54.Simon L, Emery BR, Carrell DT. Review: impact of sperm DNA damage in assisted reproduction. Best Pract Res Clin Obstet Gynaecol. https://doi.org/10.1016/j.bpobgyn.2017.07.003 (Published online ahead of print 24 July 2017).
- 56.Zheng WW, Song G, Wang QL, et al. Sperm DNA damage has a negative effect on early embryonic development following in vitro fertilization. Asian J Androl. 2017; https://doi.org/10.4103/aja.aja_19_17 (Published online ahead of print Jun 30).
- 61.Tavares RS, Silva AF, Lourenço B, Almeida-Santos T, Sousa AP, Ramalho-Santos J. Evaluation of human sperm chromatin status after selection using a modified Diff-Quik stain indicates embryo quality and pregnancy outcomes following in vitro fertilization. Andrology. 2013;1:830–7. https://doi.org/10.1111/j.2047-2927.2013.00127.x.CrossRefPubMedGoogle Scholar
- 65.Ward SW. Eight tests for sperm DNA fragmentation and their roles in the clinic. Transl Androl Urol. 2017. https://doi.org/10.21037/tau.2017.03.78 (in press, Published online ahead of print).
- 67.Ribas-Maynou J, García-Peiró A, Fernández-Encinas A, et al. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology. 2013;1:715–22. https://doi.org/10.1111/j.2047-2927.2013.00111.x.CrossRefPubMedGoogle Scholar