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Infection of defective human T-lymphotropic virus type 1

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Abstract

In a previous study, we reported that an identical defective provirus had integrated into multiple sites of the genome of a representative human T-lymphotropic virus type 1 (HTLV-1) cell line, MT-2. A possible explanation for this may be the repeated infection of this defective provirus to a cell. Therefore, we attempted to determine whether a defective provirus could transmit during the co-culture of HTLV-1 uninfected human T-cell line, Jurkat, with MT-2 cells treated with mitomycin C. As a result, we established not only a cell line with the integration of one complete provirus, but also a cell line with the integration of one defective provirus. The rearrangement of the T-cell receptor -γ gene of these cell lines showed them to be derived from Jurkat cells. Both HTLV-1 Tax/Rex and HBZ RNA were detected in the cell line, which harbors a complete provirus. On the other hand, HBZ RNA and transcriptional product specific for the defective provirus were detected in the cell line, which harbors a defective HTLV-1 provirus only. These results suggested that a defective HTLV-1 provirus with large depletion of internal sequence could transmit to other cells. Moreover, the defective provirus can be transcriptionally active. This suggested the possibility that the defective HTLV-1 provirus found in the lymphocytes of HTLV-1 carriers and patients with adult T-cell leukemia may transmit to other T-cells in vivo. The results also suggested that defective provirus in HTLV-1 carriers could be functional and may play a role in leukemogenesis.

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Acknowledgements

The authors would like to thank Ms. Y. Kaseda and Ms. K. Takatsuka (Miyazaki University) for their technical support and assistance.

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Authors and Affiliations

  1. Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

    Yuuki Hashikura, Kazumi Umeki, Kunihiko Umekita, Hajime Nomura, Akiteru Yamada, Ikuo Yamamoto & Akihiko Okayama

  2. Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8102, Japan

    Hiroo Hasegawa & Katsunori Yanagihara

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  1. Yuuki Hashikura
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  2. Kazumi Umeki
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Correspondence to Akihiko Okayama.

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Hashikura, Y., Umeki, K., Umekita, K. et al. Infection of defective human T-lymphotropic virus type 1. Human Cell 30, 117–123 (2017). https://doi.org/10.1007/s13577-016-0156-4

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  • DOI: https://doi.org/10.1007/s13577-016-0156-4

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