Abstract
Salt-tolerant variety Chuanqiao No. 1 and salt-sensitive variety Chuanqiao No. 2 of Tartary buckwheat were used as experimental materials. The effect of aspartic acid on seed germination, physiological characteristics of seedlings and gene expression of salt exclusion in Tartary buckwheat were studied under NaCl stress of 150 mM. The results showed that the aspartic acid treatment could restore the seed germination rate and root vigor of seedlings to the control with non-damage level in salt-tolerant Tartary buckwheat variety under salt stress, and the salt-sensitive variety was increased greatly. Spraying aspartic acid had some protective effects on cell membrane of leaves in Tartary buckwheat under salt stress, and the protective effects were more obviously on salt-sensitive variety, and that could restore the activity of SOD and CAT of leaves to the control level in salt-tolerant Tartary buckwheat variety under salt stress, and the activity of antioxidant enzymes in salt-sensitive variety was increased significantly. The relative expression of FtNHX1 and FtSOS1 genes was increased significantly under salt stress, and that of FtNHX1 gene in salt-tolerant and salt-sensitive varieties was reached the maximum expression level at 12 h and 24 h respectively, while that of FtSOS1 gene in salt-tolerant and salt-sensitive varieties was reached the maximum expression level at 12 h, and the salt-tolerant variety was increased greatly. After spraying aspartic acid, the relative expression of FtNHX1 and FtSOS1 genes was increased more obviously. The relative expression of FtNHX1 gene in salt-tolerant and salt-sensitive varieties was reached the maximum expression level at 12 h, while that of FtSOS1 gene was reached the maximum expression level at 12 h and 24 h respectively, and that in salt-tolerant variety was increased especially more, indicating that spraying aspartic acid on gene expression of salt exclusion in salt-tolerant variety of Tartary buckwheat has a better effect under salt stress.
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Abbreviations
- CAT:
-
Catalase
- MDA:
-
Malondialdehyde
- POD:
-
Peroxidase
- SOD:
-
Superoxide dismutase
- TTC:
-
Triphenyltetrazolium chloride
References
Apse MP, Blumwald E (2007) Na+ transport in plants. FEBS Lett 581(12):2247–2254
Blumwald E, Aharon GS, Apse MP (2000) Sodium transport in plant cells. Biochim Biophys Acta 1465(1):140–151
Cai XF, Hu TX, Ye J, Zhang YY, Li HX, Ye ZB (2015) Molecular mechanisms of salinity tolerance in plants. J Huazhong Agric Univ 34(3):134–141
Cao Y, Huang RD, Cao ZQ (2005) Effects of Pb stress on the physiological and biochemical traits of maize. J Maize Sci 13(3):61–64
Chen LH, Zhang B, Xu ZQ (2007) Salt tolerance conferred by over expression of Arabidopsis vacuolar Na+/H+ antipoter gene AtNHX1 in commom buckwheat (Fagopyrum esculentum). Transgenic Res 17(1):121–132
Dou J, Yu S, Fan S, Pang C, Song M (2000) SOD and plant stress resistance. Mol Plant Breed 8(2):359–364
Gao FY, Zhang JL, Liu ZP, Guo FF (2010) Effects of salt stress on chlorophyll content and root activity of Caragana arborescens. Guizhou Agric Sci 38(5):46–48
Gong M, Liu Y, Ding N, He Z (1994) Difference of salt tolerance in Hordeum vulgare at different growth stages. Acta Bot Boreal-Occident Sin 14(1):1–7
Han Y, Sheng YF, Luo X, Sun XC, Wang CH, Zhao GM (2014) Physiological mechanism of Carthamus tinctorius L. seedlings in response to salt stress. Chin J Ecol 33(7):1833–1838
He B, Xu H, Chen J (1997) Effects of drought stress on the permeability of plasma membrane and anti-oxidation enzymes of the leaves of sweet potato. J Guangxi Agric Univ 16(4):287–290
Hou LG, Chen WF, Ma W, Zhao GC, Qi CY, Liu L, Sun HJ (2012) Effects of different phosphate fertilizer application on permeability of membrane and antioxidative enzymes in rice under low temperature stress. Acta Agric Boreali-Sin 27(1):118–123
Hu YJ, Jiang Y, Feng LJ, Gao JF, Feng BL (2008) Study on changes of nutrient content and active ingredient of buckwheat peas. Agric Res Arid Areas 26(2):111–115
Ji PF, Chen XH, Yang HB (2014) Effects of amino acid on physiological traits of buckwheat seedlings under salt stress. Hubei Agric Sci 53(16):3749–3752
Li J, Du LF (2001) A new approach to detect plant thykaloid phosphoprotein in vivo. Prog Biochem Biophys 28(5):740–743
Li JS, Wang HC, Wang WY, Zhu YF (1983) Effect of drought on the permeability and membrane lipid composition from maize leaves. Acta Phytophysiol Sinica 9(3):223–229
Li C, Liu X, Yang Y, Liu C (2005) The effects of saline condition on the germination and seedling growth of Suaeda salsa. Chin Agric Sci Bull 21(5):209–212
Li Q, Liu JH, Wu JY, Li LJ, Zhang L (2009) Effect of salt stress on membrane permeability and Na+, K+ absorption of oat. Acta Agric Boreali-Sin 24(6):88–92
Liu HC, Jia WQ (2009) Studies on POD, CAT of white clover stems under salt stress. J Jilin Agric Sci 34(1):43–46
Liu JY, Liu X (2010) Experimental course of plant physiology. Higher Education Press, Beijing
Liu H, Yang H (2015) Physiological effects of glutamic acid and aspartic acid on buckwheat seedlings under high temperature stress. Jiangsu Agric Sci 43(1):108–110
Liu XC, Chen HF, Jing LW, Chen QZ (2009) Effects of salt stress on SOD/CAT activities and malondiadehyde (MDA) content of Triticum aestivum Thinopyrum bessarabicum diploid. Anhui Agric Sci Bull 15(8):43–46
Liu XH, Zhang YP, Zhang YH, Yang HB, Dong CH (2015) Comparison of salt tolerance among five Fagopyrum tataricum vaieties under salt stress. Hubei Agric Sci 54(17):4128–4130
Meng X (2010) Isolation and expression of the Na+/H+ antiporter gene of Malus zumi (MzNHX1) under salt stress. Tianjin Agricultural University, Tianjin
Meng X, Liu Y, Zhang Y, Zhang H, Mu P, Lin Q (2015) Responses of antioxidant properties and root activity of different wheat varieties to salt stress at seedling stage. J Triticeae Crops 35(8):1168–1175
Peng LX, Wang MQ (2005) Regulation gene of osmotic stress: Na+/H+ antiporter gene and salt tolerance of plants. J Tianjiin Agric Coll 12(2):45–47
Reguera M, Bassil E, Blumwald E (2014) Intracellular NHX-type cation/H+ antiporters in plants. Mol Plant 7(2):261–263
Shi H, Quintero FJ, Pardo JM, Zhu JK (2002) The putative plasma membrane Na+/H+ antiporter SOS1 controls long-distance Na+ transport in plants. Plant Cell 14(2):465–477
Tian XL, Guan XL, Li Y, Zhang SQ (2005) The effect of gamma-aminobutyric acid in superoxide dismutase, peroxidase and catalase activity response to salt stress in maize seedling. Acta Biol Exp Sin 38(1):75–79
Tian XY, Liu YJ, Zhang L, Zhang H, Liu PH (2009) Effects of salt stress on MDA, protective enzyme system, proline and soluble sugar of Sedum aizoon seedling. Grassland Turf 6:11–14
Wan X, Song Y (1995) Effects of salt-stress and Ca2+ regulation on bamboo root viability and MDA content. J Nanjing For Univ 19(3):16–20
Wang Y, Chen J, Di X (2011) A comparative study on the SOD, POD, MDA and dissoluble protein of six provenances of Chinese pine (Pinus abulaeformis Carr.) under water stress. Ecol Environ Sci 20(10):1449–1453
Wei H, Guo J, Zhang S, Huang B, Liu Y, Du L (2006) The presence of phosphorylation form of D1 protein in its cross-linked aggregates in high light treated spinach leaves in vivo. Chin Sci Bull 50(1):69–74
Xiao W, Jia HX, Pu LM (2000) Studies on physiological index of some halophytes. Acta Bot Boreal-Occident Sin 20(5):818–825
Xu M, Yuan L, Li W, Li YT, Li J, Zhao BQ (2018) Effects of a fertilizer synergist containing compound amino acids on seed germination and seedling growth of pakchoi under NaCl stress. J Plant Nutr Fert 24(4):992–1000
Yang H (2014a) Effects of amino acid on seed germination and seedling growth of buckwheat under salt stress. Guizhou Agric Sci 42(8):30–33
Yang HB (2014b) Effects of exogenous amino acid on seeds germination and seedlings growth of buckwheat under high temperature stress. J Henan Agric Sci 43(11):20–23
Yang HB, Yang SP (2014) Effects of salt stress on seeds germination and seedlings growth of two buckwheat cultivars. Hubei Agric Sci 53(11):2505–2507
Yang P, Zhou F, Zhang B, Wang L, Qian B, Chen Q (2016) Phylogenetic analysis and detection of selective pressure on plant salt overly sensitive genes. Mol Plant Breed 14(8):2098–2105
Zou Q (2000) Experimental guide to plant physiology. China Agricultural Press, Beijing
Acknowledgements
We would like to acknowledge the financial supports from the National Natural Science Foundation of China (31371552), and the research materials Chuanqiao Nos. 1 and 2 were furnished by Research Station of Alpine Crop, Department of Agriculture and Science in Liangshan State, Sichuan Province, China.
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J-SZ analyzed the physiological characteristics of Tartary buckwheat and wrote the manuscript. Y-QW analyzed the gene express of FtNHX1 by doing real-time PCR. J-NS analyzed the gene express of FeSOS1 by doing real-time PCR. J-PX cultivated the plant and treated. The corresponding author H-BY designed the experiment and revised the manuscript.
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Zhang, JS., Wang, YQ., Song, JN. et al. Effect of aspartic acid on physiological characteristics and gene expression of salt exclusion in Tartary buckwheat under salt stress. J. Plant Biochem. Biotechnol. 29, 94–101 (2020). https://doi.org/10.1007/s13562-019-00518-y
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DOI: https://doi.org/10.1007/s13562-019-00518-y