Physiological, biochemical, antioxidant and growth characterizations of gibberellin and paclobutrazol-treated sweet leaf (Stevia rebaudiana B.) herb

Short Communication

Abstract

A greenhouse experiment was designed to study the responses of Stevia rebaudiana herb to paclobutrazol (PBZ) and gibberellin (GA) treatments. GA and PBZ treatments caused no significant impact on photosynthesis pigments while they increased carbohydrates, amino acids and protein metabolites. Stevia showed a potent antioxidant activity through scavenging DPPH, NO·; O 2 ·− and OH· radicals which was highlighted in GA and PBZ treatments. The enzymatic and non-enzymatic antioxidant system of Stevia plant showed a significant increase in response to PBZ and GA treatments. PBZ treatment decreased plant growth while GA treatment had no significant effect on it. Collectively, both GA and PBZ treatments effectively increased metabolites and antioxidant property of Stevia herb.

Keywords

Antioxidant Gibberellin Growth Metabolites Paclobutrazol Stevia rebaudiana 

Abbreviations

DHA

Dehydroascorbate

DPPH

2,2-Diphenyl-1-picrylhydrazyl

FRAP

Ferric reducing antioxidant power

LFM

Leaf fresh mass

LWC

Leaf water content

GSH

Reduced glutathione

GSSG

Oxidized glutathione

MS

Murashige and Skoog

P5CS

Pyrroline-5-carboxylate synthetase

ROS

Reactive oxygen species

SVglys

Steviol glycosides

SOD

Superoxide dismutase

WSC

Water soluble carbohydrates

Notes

Compliance with ethical standards

Conflict of interest

The author declares that she has no conflict of interest.

References

  1. Ahmad N, Fazal H, Abbasi B, Farooq S (2010) Efficient free radical scavenging activity of Ginkgo biloba, Stevia rebaudiana and Parthenium hysterophorus leaves through DPPH (2,2-diphenyl-1-picrylhydrazyl). Int J Phytom 2:231–239Google Scholar
  2. Ceunen S, Geuns JM (2013) Steviol glycosides: chemical diversity, metabolism, and function. J Nat Prod 76:1201–1228CrossRefPubMedGoogle Scholar
  3. Gopi R, Jaleel CA, Sairam R, Lakshmanan GM, Gomathinayagam M, Panneerselvam R (2007) Differential effects of hexaconazole and paclobutrazol on biomass, electrolyte leakage, lipid peroxidation and antioxidant potential of Daucus carota L. Colloid Surf B Biointerfaces 60:180–186CrossRefPubMedGoogle Scholar
  4. Hajihashemi S, Ehsanpour A (2013) Influence of exogenously applied paclobutrazol on some physiological traits and growth of Stevia rebaudiana under in vitro drought stress. Biologia 68:414–420CrossRefGoogle Scholar
  5. Hajihashemi S, Ehsanpour A (2014) Antioxidant response of Stevia rebaudiana B. to polyethylene glycol and paclobutrazol treatments under in vitro culture. Appl Biochem Biotechnol 172:4038–4052CrossRefPubMedGoogle Scholar
  6. Hajihashemi S, Geuns JM (2013) Free radical scavenging activity of steviol glycosides, steviol glucuronide, hydroxytyrosol, metformin, aspirin and leaf extract of Stevia rebaudiana. Free Radic Antioxid 3:34–41Google Scholar
  7. Hajihashemi S, Geuns JM (2017) Steviol glycosides correlation to genes transcription revealed in gibberellin and paclobutrazol-treated Stevia rebaudiana. J Plant Biochem Biotechnol. doi: 10.1007/s13562-017-0399-5 Google Scholar
  8. Hajihashemi S, Geuns JM, Ehsanpour A (2013) Gene transcription of steviol glycoside biosynthesis in Stevia rebaudiana Bertoni under polyethylene glycol, paclobutrazol and gibberellic acid treatments in vitro. Acta Physiol Plant 35:2009–2014CrossRefGoogle Scholar
  9. Shukla S, Mehta A, Mehta P, Bajpai VK (2012) Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bert. Exp Toxicol Pathol 64:807–811CrossRefPubMedGoogle Scholar
  10. Siddiqui MH, Khan MN, Mohammad F, Khan MMA (2008) Role of nitrogen and gibberellins (GA3) in the regulation of enzyme activities and in osmoprotectant accumulation in Brassica juncea L. under salt stress. J Agron Crop Sci 194:214–224CrossRefGoogle Scholar
  11. Yang Y, Liu Q, Wang GX, Wang XD, Guo JY (2010) Germination, osmotic adjustment, and antioxidant enzyme activities of gibberellin-pretreated Picea asperata seeds under water stress. New For 39:231–243CrossRefGoogle Scholar

Copyright information

© Society for Plant Biochemistry and Biotechnology 2017

Authors and Affiliations

  1. 1.Plant Biology Department, Faculty of ScienceBehbahan Khatam Alanbia University of TechnologyBehbahanIran

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