Abstract
Objective
The purpose of this study was to observe the possible laxative effects of a triple-fermented barley (Hordeum vulgare L.) extract (FBe) obtained by saccharification and by using Saccharomyces cerevisiae and Weissella cibaria in normal rats.
Methods
Forty rats (8 rats in five groups) were selected and used after 10 d of acclimatization. FBe at concentrations of 100, 200, or 300 mg/kg was orally administered once a day for 15 d, and the changes in fecal parameters, gastrointestinal transit ratio, fecal mucus content, and colonic mucosa histology, were observed in normal rats. The laxative effects of FBe were compared against those of sodium picosulfate.
Results
By assessing the key parameters for laxative effects in rats, the present study demonstrated that oral administration of 200 and 300 mg/kg FBe showed favorable laxative effects, mediated by increases in gastrointestinal motility. However, restricted effects were observed when using 100 mg/kg FBe, at least under the experimental conditions presented here.
Conclusion
Based on these results, we conclude that FBe is promising as a new potent laxative agent or as an ingredient in functional foods, with low toxicity at a dose level of 200 mg/kg in normal rats.
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References
Kakino, M. et al. Laxative effects of agarwood on low-fiber diet-induced constipation in rats. BMC Complement. Altern. Med. 10, 68 (2010).
Jones, R. & Lydeard, S. Irritable bowel syndrome in the general population. BMJ 304, 87–90 (1992).
Talley, N. J. et al. Functional gastroduodenal disorders. Gut 45, 1137–1142 (1999).
Mostafa, S. M., Bhandari, S., Ritchie, G., Gratton, N. & Wenstone, R. Constipation and its implications in the critically ill patient. Br. J. Anaesth. 91, 815–819 (2003).
Siegers, C. P., von Hertzberg-Lottin, E., Otte, M. & Schneider, B. Anthranoid laxative abuse-a risk for colorectal cancer? Gut 34, 1099–1101 (1993).
Kim, H. S. et al. Single oral dose toxicity test of blue honeysuckle concentrate in mice. Toxicol. Res. 31, 61–68 (2015).
Devipriya, N., Srinivasan, M., Sudheer, A. R. & Menon, V. P. Effect of ellagic acid, a natural polyphenol, on alcohol induced prooxidant and antioxidant imbalance: a drug dose dependent study. Singapore Med. J. 48, 311–318 (2007).
Saravanan, N., Rajasankar, S. & Nalini, N. Antioxidant effect of 2-hydroxy-4-methoxy benzoic acid on ethanol induced hepatotoxicity in rats. J. Pharm. Pharmacol. 59, 445–453 (2007).
Noh, J. R. et al. Hepatoprotective effect of Platycodon grandiflorum against chronic ethanol-induced oxidative stress in C57BL/6 mice. Ann. Nutr. Metab. 58, 224–231 (2011).
Ruidavets, J. B. et al. High consumptions of grain, fish, dairy products and combinations of these are associated with a low prevalence of metabolic syndrome. J. Epidemiol. Community Health 61, 810–817 (2007).
Hole, A. S. et al. Activation and inhibition of nuclear factor kappa B activity by cereal extracts: role of dietary phenolic acids. J. Agric. Food Chem. 57, 9481–9488 (2009).
Giriwono, P. E., Shirakawa, H., Hokazono, H., Goto, T. & Komai, M. Fermented barley extract supplementation maintained antioxidative defense suppressing lipopolysaccharide- induced inflammatory liver injury in rats. Biosci. Biotechnol. Biochem. 75, 1971–1976 (2011).
Giriwono, P. E. et al. Extract of fermented barley attenuates chronic alcohol induced liver damage by increasing antioxidative activities. Food Res. Int. 43, 118–124 (2010).
Bae, E. A. et al. Protective effect of fermented red ginseng on a transient focal ischemic rats. Arch. Pharm. Res. 27, 1136–1140 (2004).
Trinh, H. T., Han, S. J., Kim, S. W., Lee, Y. C. & Kim, D. H. Bifidus fermentation increases hypolipidemic and hypoglycemic effects of red ginseng. J. Microbiol. Biotechnol. 17, 1127–1133 (2007).
Jung, Y. M. et al. Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects. Nutr. Res. 31, 387–396 (2011).
Kim, C. M., Yi, S. J., Cho, I. J. & Ku, S. K. Red-koji fermented red ginseng ameliorates high fat diet-induced metabolic disorders in mice. Nutrients 5, 4316–4332 (2013).
Choi, J.-S. et al. Laxative effects of fermented rice extract (FRe) in normal rats. Toxicol. Environ. Health Sci. 6, 155–163 (2014a).
Choi, J.-S. et al. Laxative effects of fermented rice extract in rats with loperamide-induced constipation. Exp. Ther. Med. 8, 1847–1854 (2014).
Choi, J.-S. et al. Synergistic effect of fermented rice extracts on the probiotic and laxative properties of yoghurt in rats with loperamide-induced constipation. Evid. Based. Complement. Alternat. Med. 2014, 878503 (2014).
Choi, J.-S., Kim, J. W., Kim, K. Y., Ku, S. K. & Sohn, J. H. Single-dose oral toxicity of fermented rice extracts (FREs): a 14-day observation. Pak. J. Pharm. Sci. 27, 129–137 (2014c).
Robles-Escajeda, E. et al. Searching in mother nature for anti-cancer activity: anti-proliferative and pro-apoptotic effect elicited by green barley on leukemia/lymphoma cells. PLoS One 8, e73508 (2013).
Mattila, P., Pihlava, J. M. & Hellström, J. Contents of phenolic acids, alkyl- and alkenylresorcinols, and avenanthramides in commercial grain products. J. Agric. Food Chem. 53, 8290–8295 (2005).
Pérez-Jiménez, J. & Saura-Calixto, F. Literature data may underestimate the actual antioxidant capacity of cereals. J. Agric. Food Chem. 53, 5036–5040 (2005).
Charalampopoulos, D., Pandiella, S. S. & Webb, C. Evaluation of the effect of malt, wheat and barley extracts on the viability of potentially probiotic lactic acid bacteria under acidic conditions. Int. J. Food. Microbiol. 82, 133–141 (2003).
Ye, X., Morimura, S., Han, L. S., Shigematsu, T. & Kida, K. JIn vitro evaluation of physiological activity of vinegar produced from barley-, sweet potato-, and riceshochu post-distillation slurry. Biosci. Biotechnol. Biochem. 68, 551–556 (2004).
Yoshimoto, M. et al. Phenolic composition and radical scavenging activity of sweetpotato-derived shochu distillery by-products treated with koji. Biosci. Biotechnol. Biochem. 68, 2477–2483 (2004).
Iguchi, T., Kawata, A., Watanabe, T., Mazumder, T. K. & Tanabe, S. Fermented barley extract suppresses the development of atopic dermatitis-like skin lesions in NC/Nga mice, probably by inhibiting inflammatory cytokines. Biosci. Biotechnol. Biochem. 73, 489–493 (2009).
Hokazono, H., Omori, T. & Ono, K. Effects of single and combined administration of fermented barley extract and gamma-aminobutyric acid on the development of atopic dermatitis in NC/Nga mice. Biosci. Biotechnol. Biochem. 74, 135–139 (2010).
Kim, H. B. et al. Ethanol extract of fermented soybean, Chungkookjang, inhibits the apoptosis of mouse spleen, and thymus cells. J. Microbiol. 45, 256–261 (2007).
Leng-Peschlow, E. Effect of sennosides and related compounds on intestinal transit in the rat. Pharmacology 36, 40–48 (1988).
Rumsey, R. D., Squires, P. E. & Read, N. W. In vitro effects of sennoside on contractile activity and fluid flow in the perfused large intestine of the rat. Pharmacology 47, 32–39 (1993).
Lee, J. K. et al. Synergistic laxative effect of fermented rice extracts with probiotic-containing yogurt in normal rats. Toxicol. Environ. Health Sci. 6, 87–98 (2014).
Forth, W., Nell, G., Rummel, W. & Andres, H. The hydrogogue and laxative effect of the sulfuric acid ester and the freediphenol of 4,4ʹ-dihydroxydiphenyl-(pyridyl-2)-methane. Naunyn-Schmiedeberg’s Arch. Pharmacol. 274, 46–53 (1972).
Jauch, R., Hankwitz, R., Beschke, K. & Pelzer, H. Bis-(p-hydroxyphenyl)-pyridyl-2-methane: the common laxative principle of bisacodyl and sodium picosulphate. Arzneimittelforschung 25, 1796–1800 (1975).
Méité, S. et al. Laxative activities of Mareya micrantha (Benth.) Müll. Arg. (Euphorbiaceae) leaf aqueous extract in rats. BMC Complement. Altern. Med. 10, 7 (2010).
Wintola, O. A., Sunmonu, T. O. & Afolayan, A. J. The effect of Aloe ferox Mill. in the treatment of loperamideinduced constipation in Wistar rats. BMC Gastroenterol. 10, 95 (2010).
Fox, J. G., Cohen, B. J. & Loew, F. M. in Laboratory animal medicine (Academic Press. Inc., Orlando, 1984).
Tajima, Y. in Biological reference data book on experimental animals (Soft Science Inc., Tokyo, 1989).
Wu, D. et al. Traditional Chinese formula, lubricating gut pill, stimulates cAMP-dependent Cl (-) secretion across rat distal colonic mucosa. J. Ethnopharmacol. 134, 406–413 (2011).
Yang, Z. H. et al. Cellular mechanisms underlying the laxative effect of flavonol naringenin on rat constipation model. PLoS One 3, e3348 (2008).
Sagar, L., Sehgal, R. & Ojha, S. Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice. BMC Complement. Altern. Med. 5, 18 (2005).
McCullogh, J. S., Ratcliffe, B., Mandir, N., Carr, K. E. & Goodlad, R. A. Dietary fibre and intestinal microflora: effects on intestinal morphometry and crypt branching. Gut 42, 799–806 (1998).
Levene, A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin. Otolaryngol. Allied Sci. 6, 145–151 (1981).
Ludbrook, J. Update: microcomputer statistics packages. A personal view. Clin. Exp. Pharmacol. Physiol. 24, 294–296 (1997).
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Lim, JM., Song, CH., Park, SJ. et al. Laxative Effects of Triple-Fermented Barley Extracts (Fbe) in Normal Rats. Toxicol. Environ. Health Sci. 10, 107–117 (2018). https://doi.org/10.1007/s13530-018-0353-5
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DOI: https://doi.org/10.1007/s13530-018-0353-5