Skip to main content
SpringerLink
Log in

Effects of Silicon in the Amelioration of Zn Toxicity on Antioxidant Enzyme Activities

  • Published:
Toxicology and Environmental Health Sciences Aims and scope Submit manuscript

Abstract

Objective

Silicon, an abundant element in the earth’s crust, is a known factor in reducing the toxicity of plants. The effects of silicon were investigated to the amelioration of Zinc (Zn) toxicity on antioxidant enzyme activities (Superoxide dismutase (SOD), Catalase (CAT), and Glutathione Reductase (GR)), Hydrogen peroxide concentrations (H2O2), phenylalanine ammonia-lyase (PAL), and soluble protein (SP) in one bamboo species (Arundinaria pygmaea).

Methods

This study was conducted in vitro condition to determine the effects of four Zn concentrations (100, 300, 500, and 1000 µmol/L) at two different concentrations of silicon (Si) (0 and 100 µmol/L) on a single bamboo species (Arundinaria pygmaea).

Results

The results indicated that Si can stimulate the plant defense mechanism and ameliorate heavy metal stress caused by Zn concentrations, which can increase antioxidant enzyme and non-enzyme activity and decrease damaging effects caused by free radicals, H2O2, and soluble protein in this bamboo species.

Conclusion

Furthermore, the results indicated that the combination of 100/300 µmol/L had a considerable impact on the reduction of Zn toxicity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Hartley, S. E. & DeGabri, J. L. The ecology of herbivore- induced silicon defences in grasses. Funct. Ecol. 30, 1311–1322 (2016).

    Article  Google Scholar 

  2. Epstein, E. Silicon. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 641–664 (1999).

    CAS  Google Scholar 

  3. Sommer, M., Kaczorek, D., Kuzyakov, Y. & Breuer, J. Silicon pools and fluxes in soils and landscapes-a review. J. Plant Nut. Soil Sci. 169, 310–329 (2006).

    Article  CAS  Google Scholar 

  4. Rogalla, H. & Romheld, V. Role of leaf apoplast in silicon- mediated manganese tolerance of Cucumis stivus L. Plant Cell Environ. 25, 549–555 (2002).

    Article  CAS  Google Scholar 

  5. Zhu, Z., Wei, G., Li, J., Qian, Q. & Yu, J. Silicon aleviated salt stress and increases antioxidant enzymes activity in leaves of salt stresses cucumber (Cucumis sativus L). Plant Sci. 167, 527–533 (2004).

    Article  CAS  Google Scholar 

  6. Kim, Y. H., Khan, A. L., Waqas, M. & Lee, I. J. Silicon regulates Antioxidant Activities of crops plant under Abiotic-Induced Oxidative streses A review. Front. Plant Sci. 6, 510 (2017).

    Google Scholar 

  7. Alexandre, A., Meunier, J. D., Lézine, A. M., Vincens, A. & Schwartz, D. Phytoliths: indicators of grassland dynamics during the late Holocene in intertropical Africa. Palaeogeogr. Palaeoclimatol. Palaeoecol. 136, 213–229 (1997).

    Article  Google Scholar 

  8. Ding, T. P. et al. Silicon isotope fractionation in bamboo and its significance to the biogeochemical cycle of silicon. Geochim. Cosmochim. Acta 72, 1381–1395 (2008).

    Article  CAS  Google Scholar 

  9. Richmond, K. E. & Sussman, M. Got Silicon? The non essential benefit plant nutrreant. Curr. Opin. Plant Biol. 6, 268–272 (2003).

    Article  PubMed  CAS  Google Scholar 

  10. Mitani, N. & Ma, J. F. Uptake system of silicon in different plant species. J. Exp. Bot. 56, 1255–1261 (2005).

    Article  PubMed  CAS  Google Scholar 

  11. Pallavi, S., Ambuj, B. J., Rama, S. D. & Mohammad, P. Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions. J. Bot. 217037, doi: 10.1155/2012/217037 (2012).

    Google Scholar 

  12. Guan, F., Tang, X., Fan, S., Zhao, J. & Peng, C. Changes in soil carbon and nitrogen stocks followed the conversion from secondary forest to Chinese fir and Moso bamboo plantations. CATENA 133, 455–460 (2015).

    Article  CAS  Google Scholar 

  13. Yang, Q. et al. Bamboo resources, utilization and exsitu conservation in Xishuangbanna, South-eastern China. J. Forest. Res. 19, 79–83 (2008).

    Article  Google Scholar 

  14. Huang, Y. P., Hou, C. H., Hsi, H. C. & Wu, J. W. Optimization of highly microporous activated carbon preparation from Moso bamboo using central composite design approach. J. Taiwan Inst. Chem. Eng. 50, 266–275 (2015).

    Article  CAS  Google Scholar 

  15. Hogarth, N. J. & Belcher, B. The contribution of bamboo to household income and rural livelihoods in a poor and mountainous county in Guangxi, China. Int. Forest. Rev. 15, 71–81 (2013).

    Article  Google Scholar 

  16. Zhang, X., Zhong, T., Liu, L. & Ouyang, X. Impact of Soil Heavy Metal Pollution on Food Safety in China. PLoS ONE 10, e0135182 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  17. Mittler, R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405–410 (2002).

    Article  PubMed  CAS  Google Scholar 

  18. Shi, Q. et al. Silicon-mediated alleviation of Mn toxicity in Cucumis sativus in relation to activities of superoxide dismutase and ascorbate peroxidase. Phytochemistry 66, 1551–1559 (2005).

    Article  PubMed  CAS  Google Scholar 

  19. Takahashi, M. A. & Asada, K. Superoxide anion primeability of phospholipid membranes and chloroplast thylakoides. Arch. Biochem. Biophys. 226, 558–566 (1983).

    Article  PubMed  CAS  Google Scholar 

  20. Song, A. et al. The alleviation of zinc toxicity by silicon is related to zinc transport and antioxidative reactions in rice. Plant Soil 344, 319–333 (2011).

    Article  CAS  Google Scholar 

  21. Sivanesan, I. & Jeong, B. R. Silicon promotes adventitious shoot regeneration and enhances salinity tolerance of Ajuga multiflora Bunge by altering activity of antioxidant enzyme. Sci. World J. 521703, http://dx.doi.org/10.1155/2014/521703 (2014).

    Google Scholar 

  22. Feng, J. P., Shi, Q. H. & Wang, X. F. Effect of exogenoud silicon on photosyntesis capacity and antioxidant anzyme activity in choloroplast of cucumber seedling under excess maneges. Agric. Sci. China 8, 40–50 (2009).

    Article  CAS  Google Scholar 

  23. Tripathi, D. K., Singh, V. P., Prasad, S. M., Chauhan, D. K. & Dubey, N. K. Silicon nanoparticles (SiNp) alleviate chromium (VI) phytotoxicity in Pisum sativum (L.) seedlings. Plant Physiol. Biochem. 96, 189–198 (2015).

    Article  PubMed  CAS  Google Scholar 

  24. Farooq, M. A. et al. Alleviation of cadmium toxicity by silicon is related to elevated photosynthesis, antioxidant enzymes; suppressed cadmium uptake and oxida tive stress in cotton. Ecotoxicol. Environ. Saf. 96, 242–249 (2013).

    Article  PubMed  CAS  Google Scholar 

  25. Song, A. et al. Silicon-enhanced resistance to cadmium toxicity in Brassica chinensis L. is attributed to Si-suppressed cadmium uptake and transport and Si-enhanced antioxidant defense capacity. J. Hazard. Mater. 172, 74–83 (2009).

    Article  PubMed  CAS  Google Scholar 

  26. Shi, G., Cai, Q., Liu, C. & Li, W. Silicon alleviates cadmium toxicity in peanut plants in relation to cadmium distribution and stimulation of antioxidative enzymes. Plant Growth Regul. 61, 45–52 (2010).

    Article  CAS  Google Scholar 

  27. Tang, H. et al. Effects of selenium and silicon on enhancing antioxidative capacity in ramie (Boehmeria nivea (L.) Gaud.) under cadmium stress. Environ. Sci. Pollut. Res. Int. 22, 9999–10008 (2015).

    Article  PubMed  CAS  Google Scholar 

  28. Molassiotis, A., Sotiropoulos, T., Tanou, G., Diamantidis, G. & Therios, I. Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM9 (Malus domestica Borkh). Environ. Exper. Bot. 56, 54–62 (2006).

    Article  CAS  Google Scholar 

  29. Gunes, A., Inal, A., Bagci, E. G., Coban, S. & Pilbeam, D. J. Silicon mediates changes to some physiological and enzymatic parameters symptomatic for oxidative stress in spinach (Spinacia oleracea L.) grown under B toxicity. Sci. Hort. 113, 113–119 (2007).

    Article  CAS  Google Scholar 

  30. Khandekar, S. & Leisner, S. Soluble silicon modulates expression of Arabidopsis thaliana genes involved in copper stress. J. Plant Physiol. 168, 699–705 (2011).

    Article  PubMed  CAS  Google Scholar 

  31. Li, L. et al. Silicate-mediated alleviation of Pb toxicity in banana grown in Pb-contaminated soil. Biol. Trace. Elem. Res. 145, 101–108 (2012).

    Article  PubMed  CAS  Google Scholar 

  32. Miao, B. H., Han, X. G. & Zhang, W. H. The ameliorative effect of silicon on soybean seedlings grown in potassium-deficient medium. Ann. Bot. 105, 967–973 (2010).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  33. Hall, J. L. Cellular mechanisms for heavy metal detoxification and tolerance. J. Exp. Bot. 53, 1–11 (2002).

    Article  PubMed  CAS  Google Scholar 

  34. Adrees, M. et al. Mechanisms of silicon-mediated alleviation of heavy metal toxicity in plants: A review. Ecotoxicol. Environ. Saf. 119, 186–197 (2015).

    Article  PubMed  CAS  Google Scholar 

  35. Leyva, A., Jarillo, J. A., Salinas, J. & Martinez-Zapater, J. M. Low Temperature Induces the Accumulation of Phenylalanine Ammonia-Lyase and Chalcone Synthase mRNAs of Arabidopsis thaliana in a Light-Dependent Manner. Plant Physiol. 108, 39–46 (1995).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  36. Sanchez-Ballesta, M. T., Zacarias, L., Granell, A. & Lafuente, M. T. Accumulation of PAL transcript and PAL activity as affected by heat-conditioning and low-temperature storage and its relation to chilling sensitivity in mandarin fruits. J. Agric. Food Chem. 48, 2726–2731 (2000).

    Article  PubMed  CAS  Google Scholar 

  37. Solecka, D. & Kacperska, A. Phenylpropanoid deficiency affects the course of plant acclimation to cold. Physiol. Plant 119, 253–262 (2003).

    Article  CAS  Google Scholar 

  38. Sgarbi, E., Fornasiero, R. B., Lins. A. P. & Bonatti, P. M. Phenol metabolism is differentially affected by ozone in two cell lines from grape (Vitis vinifera L.) leaf. Plant Sci. 165, 951–957 (2003).

    Article  CAS  Google Scholar 

  39. Zhang, X. The Measurement and Mechanism of Lipid Peroxidation and SOD, POD and CAT Activities in Biological System. In Research Methodology of Crop Physiology (Agriculture Press, Beijing, China, 1992).

    Google Scholar 

  40. Aebi, H. Catalase in vitro. Methods Enzymol. 105, 121–126 (1984).

    Article  PubMed  CAS  Google Scholar 

  41. Bradford, M. M. A rapid sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-Dye Binding. Anal. Biochem. 72, 248–254 (1976).

    Article  PubMed  CAS  Google Scholar 

  42. Zhang, Z. L. Experiment instruction of plant physiology (Higher Education Publishers, Beijing, 2005).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China

    Abolghassem Emamverdian, Yulong Ding & Yinfeng Xie

  2. College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China

    Abolghassem Emamverdian & Yinfeng Xie

  3. Bamboo Research Institute, Nanjing Forestry University, Nanjing, 210037, China

    Yulong Ding

Authors
  1. Abolghassem Emamverdian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yulong Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yinfeng Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yulong Ding.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Emamverdian, A., Ding, Y. & Xie, Y. Effects of Silicon in the Amelioration of Zn Toxicity on Antioxidant Enzyme Activities. Toxicol. Environ. Health Sci. 10, 90–96 (2018). https://doi.org/10.1007/s13530-018-0351-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13530-018-0351-7

Keywords

Search

Navigation