Sublethal concentrations of atrazine promote molecular and biochemical changes in the digestive gland of the Pacific oyster Crassostrea gigas
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Atrazine is an herbicide used to control pre- and post-emergence broadleaf weeds and grasses. To understand the acute effects of atrazine on oyster digestive gland, a series of molecular and biochemical responsive parameters were analyzed in the Pacific oyster Crassostrea gigas after exposure to different concentrations (1, 10, and 50 μg L-1) for 96 h. Intracellular malondialdehyde (MDA) level was significantly increased in the 50 μg L-1 exposed oysters, while no significant modulation was observed in glutathione (GSH) level. Of antioxidant defense system, enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) were significantly higher at 50 μg L-1 compared to the control or DMSO treated groups. Regarding their transcriptional levels, two glutathione S-transferases (GSTs) GSTO and GSTP, CuZnSOD, MnSOD, and CAT were significantly upregulated at 10 and/or 50 μg L-1 in the oyster digestive gland. Atrazine exposure reduced both enzymatic activity and mRNA expression of Na+/K+-ATPase and acetylcholinesterase (AChE) at 50 μg L-1. In addition, acute exposure of 10 and/or 50 μg L-1 atrazine significantly increased transcriptional expression of Hsp superfamily with strong induction of hsp70 and hsp90 family. These results suggest that atrazine has detrimental effects on the digestive gland of C. gigas by modulating important molecular and biochemical parameters within relatively short time period.
KeywordsAtrazine Pacific oyster Crassostrea gigas Digestive gland
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- 4.MDDEP. Critère de qualité de l’eau de surface. Direction du suivi de l’état de l’environnement, ministère du Développement durable, de l’Environnement et des Parcs, Québec (2008).Google Scholar
- 5.US EPA. Overview of the Ecological Risk Assessment Process in the Office of Pesticide Programs. Office of Prevention, Pesticides and Toxic Substances, Washington, DC (2004).Google Scholar
- 20.Lucu, C. & Flik, G. Na+-K+-ATPase and Na+/Ca2+ exchange activities in gills of hyperregulating Carcinus maenas. Am. J. Physiol. 276, 490–499 (1999).Google Scholar
- 34.Rhee, J.-S. et al. Effect of pharmaceuticals exposure on acetylcholinesterase (AchE) activity and on the expression of AchE gene in the monogonont rotifer, Brachionus koreanus. Comp. Biochem. Physiol. C 158, 216–224 (2013).Google Scholar
- 37.Béguel, J. P., Huvet, A., Quillien, V., Lambert, C. & Fabioux, C. Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment. Comp. Biochem. Physiol. C 157, 63–71 (2013).Google Scholar