Sublethal concentrations of atrazine promote molecular and biochemical changes in the digestive gland of the Pacific oyster Crassostrea gigas

  • Do-Hee Lee
  • Ye-Ji Rhee
  • Kwang-Sik Choi
  • Sang-Eun Nam
  • Hye-Jin Eom
  • Jae-Sung Rhee
Original Article

Abstract

Atrazine is an herbicide used to control pre- and post-emergence broadleaf weeds and grasses. To understand the acute effects of atrazine on oyster digestive gland, a series of molecular and biochemical responsive parameters were analyzed in the Pacific oyster Crassostrea gigas after exposure to different concentrations (1, 10, and 50 μg L-1) for 96 h. Intracellular malondialdehyde (MDA) level was significantly increased in the 50 μg L-1 exposed oysters, while no significant modulation was observed in glutathione (GSH) level. Of antioxidant defense system, enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) were significantly higher at 50 μg L-1 compared to the control or DMSO treated groups. Regarding their transcriptional levels, two glutathione S-transferases (GSTs) GSTO and GSTP, CuZnSOD, MnSOD, and CAT were significantly upregulated at 10 and/or 50 μg L-1 in the oyster digestive gland. Atrazine exposure reduced both enzymatic activity and mRNA expression of Na+/K+-ATPase and acetylcholinesterase (AChE) at 50 μg L-1. In addition, acute exposure of 10 and/or 50 μg L-1 atrazine significantly increased transcriptional expression of Hsp superfamily with strong induction of hsp70 and hsp90 family. These results suggest that atrazine has detrimental effects on the digestive gland of C. gigas by modulating important molecular and biochemical parameters within relatively short time period.

Keywords

Atrazine Pacific oyster Crassostrea gigas Digestive gland 

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Copyright information

© Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Do-Hee Lee
    • 1
  • Ye-Ji Rhee
    • 1
  • Kwang-Sik Choi
    • 1
  • Sang-Eun Nam
    • 1
  • Hye-Jin Eom
    • 1
  • Jae-Sung Rhee
    • 1
    • 2
    • 3
  1. 1.Department of Marine Science, College of Natural SciencesIncheon National UniversityIncheonRepublic of Korea
  2. 2.Research Institute of Basic SciencesIncheon National UniversityIncheonRepublic of Korea
  3. 3.Institute of Green Environmental Research CenterIncheonRepublic of Korea

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