Co-production of microbial lipids and biosurfactant from waste office paper hydrolysate using a novel strain Bacillus velezensis ASN1


Microbial lipids and biosurfactant were successfully co-produced using waste office paper (WOP) as a substrate using a novel strain Bacillus velezensis ASN1. WOP was pretreated with 1% (v/v) H2SO4 and enzymatically hydrolyzed using cellulase (37 FPU/g solid) and β-glucosidase (25 CBU/g solid). The total sugar yield was 21.28 g/L at 120 h and the percentage of hydrolysis was 89.07%. The obtained WOP hydrolysate was used as a substrate for the co-production of microbial lipids and biosurfactant. The best nitrogen source and carbon to nitrogen ratio for lipid production was found to be ammonium chloride and C/N 20, respectively. A lipid content of 31% was obtained with a fatty acid profile of myristic, pentadecanoic, palmitic, and stearic acid methyl esters. The crude biosurfactant was extracted by acid precipitation of the supernatant yielded 0.818 g/L and was characterized using NMR, FTIR, and LC–MS, and proved as lipopeptides. The biorefinery approach of co-producing of microbial lipid and biosurfactant using waste paper would reduce the production cost of biodiesel.

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The authors would like to thank Dr. Samuel Premkumar, Dr. Sanket Joshi and Sathish Babu, the central applied and analytical research unit (CAARU), SQU for the NMR, DSA and GC–MS analysis.In addition, authors are also thankful to Dr. Jamal Al-Sabahi, the central analytical lab, CAMS, SQU.


The authors gratefully acknowledge the Research Council (TRC), Oman, for the financial support (ORG/EBR/14/003).

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Correspondence to Nallusamy Sivakumar.

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Nair, A.S., Al-Bahry, S. & Sivakumar, N. Co-production of microbial lipids and biosurfactant from waste office paper hydrolysate using a novel strain Bacillus velezensis ASN1. Biomass Conv. Bioref. 10, 383–391 (2020).

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  • Bacillus velezensis
  • Microbial lipids
  • Biosurfactant
  • Co-production
  • Waste office paper