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Arabian Journal for Science and Engineering

, Volume 43, Issue 7, pp 3485–3491 | Cite as

Chemical Optimization of Red Pigment, Monascorubin Production in Penicillium minioluteum ED24 Using Solid-State Fermentation

  • Leong Chean Ring
  • Tong Woei Yenn
  • Najwa Izzati binti Nazri
  • Tan Wen Nee
  • Khairul Azly Zahan
  • Darah Ibrahim
  • Syarifah Rashid
Research Article - Biological Sciences
  • 42 Downloads

Abstract

Natural pigments produced as metabolites of the microorganisms are a crucial alternative to potentially harmful synthetic dyes. Unlike the traditional pigments extracted from plants and animal skin, microbial pigments are more prevalent for its availability, low-cost extraction and effectiveness. Penicillium minioluteum ED24 is an endophytic fungi previously isolated from Orthosiphon stamineus Benth and was found to produce a red pigment, monascorubin. In the present study, we investigated the feasibility of monascorubin production in P. minioluteum ED24 via solid-state fermentation (SSF). Using brown rice powder with the optimized process parameters, including 60% initial moisture content, 10%v/w \(\hbox {K}_{2}\hbox {HPO}_{4}\) and 10%v/w trace ion solution, a pigment yield of 4.89 mg/g of dry fermented substrate was obtained. Pigment yield using 1% of soluble starch and mycological peptone as additional carbon and nitrogen, respectively, significantly exceeded those of other carbon and nitrogen sources. Our result indicates the viability of brown rice in combination with P. minioluteum ED24 for industrial application through solid-state fermentation.

Keywords

Red pigment Penicillium minioluteum ED24 Solid-state fermentation Chemical optimization Endophytic fungi Monascorubin 

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Notes

Acknowledgements

The authors are grateful for the financial support from the Ministry of Education Malaysia under the Fundamental Research Grant Scheme (FRGS/1/2017/STG03/UNIKL/02/1).

References

  1. 1.
    Narsing, R.M.P.; Xiao, M.; Li, W.: J: Fungal and bacterial pigments: secondary metabolites with wide applications. Front. Microbiol. 8, 1113 (2017)CrossRefGoogle Scholar
  2. 2.
    Venil, C.K.; Zakaria, Z.A.; Ahmad, W.A.: Bacterial pigments and their applications. Process Biochem. 48, 1065–1079 (2013)CrossRefGoogle Scholar
  3. 3.
    Downham, A.; Collins, P.: Coloring our foods in the last and next millennium. Int. J. Food Sci. Technol. 35, 5–22 (2000)CrossRefGoogle Scholar
  4. 4.
    Babitha, S.; Sandhya, C.; Pandey, A.: Natural food colorants. Appl. Bot. Abstr. 23(4), 258–266 (2004)Google Scholar
  5. 5.
    Feng, Y.; Shao, Y.; Chen, F.: Monascus pigments. Appl. Microbiol. Biotechnol. 96, 1421–1440 (2012)CrossRefGoogle Scholar
  6. 6.
    Dufosse, L.; Fouillaud, M.; Caro, Y.; Mapari, S.A.; Sutthiwong, N.: Filamentous fungi are large-scale producers of pigments and colorants for the food industry. Curr. Opin. Biotechnol. 26, 56–61 (2014)CrossRefGoogle Scholar
  7. 7.
    Yenn, T.W.; Ngim, A.S.; Ibrahim, D.; Zakaria, L.: Antimicrobial activity of Penicillium minioluteum ED24, an endophytic fungus residing in Orthosiphon stamineus benth. World J. Pharm. Pharm. Sci. 3(3), 121–132 (2014)Google Scholar
  8. 8.
    Behera, S.S.; Ray, R.C.: Solid state fermentation for production of microbial cellulases: recent advances and improvement strategies. Int. J. Biol. Macromol. 86, 656–669 (2016)CrossRefGoogle Scholar
  9. 9.
    Carvalho, J.C.; Oishi, B.O.; Woiciechowski, A.L.; Pandey, A.; Babitha, S.; Socco, C.R.: Effect of substrates on the production of Monascus biopigments by solid-state fermentation and pigment extraction using different solvents. Indian J. Biotechnol. 6, 194–199 (2007)Google Scholar
  10. 10.
    Valera, H.R.; Gomes, J.; Lakshmi, S.; Gururaja, R.; Suryanarayan, S.; Kumar, D.: Lovastatin production by solid state fermentation using Aspergillus flavipes. Enzyme Microb. Technol. 37(5), 521–526 (2005)CrossRefGoogle Scholar
  11. 11.
    Krishna, C.: Solid-state fermentation systems—an overview. Crit. Rev. Biotechnol. 1(25(1–2)), 1–30 (2005)CrossRefGoogle Scholar
  12. 12.
    Pansuriya, R.C.; Singhal, R.S.: Response surface methodology for optimization of production of lovastatin by solid state fermentation. Braz. J. Microbiol. 41(1), 164–172 (2010)CrossRefGoogle Scholar
  13. 13.
    Kumar, D.; Jain, V.K.; Shanker, G.; Srivastava, A.: Utilisation of fruits waste for citric acid production by solid state fermentation. Process Biochem. 38(12), 1725–1729 (2003)CrossRefGoogle Scholar
  14. 14.
    Rasool, A.; Irum, S.: Toxic metal effect on filamentous fungi isolated from the contaminated soil of Multan and Gujranwala. J. Bioresour. Manag. 1(2), 1 (2014)Google Scholar
  15. 15.
    Ogbonna, C.N.: Production of food colorants by filamentous fungi. Afr. J. Microbiol. Res. 10(26), 960–971 (2016)CrossRefGoogle Scholar
  16. 16.
    Cho, Y.J.; Park, J.P.; Hwang, H.J.; Kim, S.W.; Choi, J.W.; Yun, J.W.: Production of red pigment by submerged culture of Paecilomyces sinclairii. Lett. Appl. Microbiol. 35(3), 195–202 (2002)CrossRefGoogle Scholar
  17. 17.
    Su, W.T.; Tsou, T.Y.; Liu, H.L.: Response surface optimization of microbial prodigiosin production from Serratia marcescens. J. Taiwan Inst. Chem. Eng. 42(2), 217–22 (2011)CrossRefGoogle Scholar

Copyright information

© King Fahd University of Petroleum & Minerals 2018

Authors and Affiliations

  • Leong Chean Ring
    • 1
  • Tong Woei Yenn
    • 1
  • Najwa Izzati binti Nazri
    • 1
  • Tan Wen Nee
    • 2
  • Khairul Azly Zahan
    • 1
  • Darah Ibrahim
    • 3
  • Syarifah Rashid
    • 4
  1. 1.Malaysian Institute of Chemical and Bioengineering TechnologyUniversiti Kuala LumpurAlor GajahMalaysia
  2. 2.School of Distance EducationUniversiti Sains Malaysia (USM)GelugorMalaysia
  3. 3.Industrial Biotechnology Research Laboratory, School of Biological SciencesUniversiti Sains MalaysiaMindenMalaysia
  4. 4.Institute of Food Security and Sustainable AgricultureUniversity Malaysia KelantanPengkalan ChepaMalaysia

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