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Pectin-encrusted gold nanocomposites containing phytic acid and jacalin: 1,2-dimethylhydrazine-induced colon carcinogenesis in Wistar rats, PI3K/Akt, COX-2, and serum metabolomics as potential targets

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Abstract

Phytic acid (PA) has momentous chemotherapeutic potential. Due to the chelate formation and rapid elimination, it is not popular in cancer treatment. The present work was inquested to develop a surface-modified nanoformulation of PA which prevents its speedy elimination and maximizes chemotherapeutic action. Chloroauric acid was reduced with pectin to produce pectin-gold nanoparticles (PGNPs). PGNPs were incubated with PA and jacalin for drug loading and surface modifications, respectively, to form PA-loaded jacalin-pectin-gold nanoparticles (PA-J-PGNPs). Formulation(s) were assessed for various pharmaceutical/pharmacological parameters. To validate the efficacy against colon carcinogenesis, formulation(s) were assessed in 1,2-dimethylhydrazine (DMH)-treated Wistar rats. DMH treatment distorted colonic architecture, oxidative, and hemodynamic parameters, which were favorably restored by PA-J-PGNP administration. To further confirm our deliberations, formulation(s) were also examined against DMH-altered metabolic changes and expression of markers pertaining to cellular proliferation, which was reinstated by PA-J-PGNPs. Our findings establish PA formulation(s) as a promising approach for suppression of colon carcinogenesis.

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Acknowledgments

The authors are grateful to Dr. Dinesh Kumar and Mr. Amit Kumar, CBMR, SGPGIMS Campus Lucknow for their help in NMR data acquisition. The University Grants Commission, Government of India granted a senior research fellowship to MA (F1-17.1/2014-15/RGNF-2014-15-SC-UTT-70422) and PP (F1-17.1/2014-15/RGNF-2014-15-SC-UTT-63524), Department of Science and Technology (DST), Government of India, DST INSPIRE Faculty Award (Ref. No. DST/Inspire Faculty Award 2014/LSBM-120) to AG, and ICMR (Ref. no.74/1/2017-Pers (EMS)) to KPG and SAS.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (BBAU), A Central University, Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India

    Malti Arya, Pooja Singh, Chandra B. Tripathi, Poonam Parashar, Mahendra Singh, Jovita Kanoujia, Gaurav Kaithwas, Krishna P. Gupta & Shubhini A. Saraf

  2. Centre of Biomedical Research (CBMR), Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS) Campus, Raebareli Road, Lucknow, UP, 226014, India

    Anupam Guleria

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  1. Malti Arya
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  2. Pooja Singh
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  3. Chandra B. Tripathi
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  4. Poonam Parashar
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  5. Mahendra Singh
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  10. Shubhini A. Saraf
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Correspondence to Shubhini A. Saraf.

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Arya, M., Singh, P., Tripathi, C.B. et al. Pectin-encrusted gold nanocomposites containing phytic acid and jacalin: 1,2-dimethylhydrazine-induced colon carcinogenesis in Wistar rats, PI3K/Akt, COX-2, and serum metabolomics as potential targets. Drug Deliv. and Transl. Res. 9, 53–65 (2019). https://doi.org/10.1007/s13346-018-00605-y

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