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VirusDisease

, Volume 30, Issue 2, pp 307–310 | Cite as

A mini outbreak of human metapneumovirus infection with severe acute respiratory symptoms in a selected group of children presented to a teaching hospital in Sri Lanka

  • Faseeha NoordeenEmail author
  • F. N. Nagoor Pitchai
  • S. Thushara Kudagammana
  • R. A. Mohamed Rafeek
Short Communication

Abstract

Human metapneumovirus (hMPV) of the family Paramyxoviridae is a relatively new virus causing severe acute respiratory tract infections (SARI) in children. Data on hMPV infection in Asia including Sri Lanka is limited. We aimed to detect respiratory viruses including hMPV in a selected group of children affected by a small outbreak of SARI presented to the Teaching Hospital, Peradeniya (THP), Sri Lanka in 2014. Nasopharyngeal aspirates (NPA) were obtained from 21 children with SARI and tested for hMPV, influenza A and B, parainfluenza 1, 2 and 3 (PIV 1–3), adenovirus and respiratory syncytial virus (RSV) antigens using an immunofluorescence assay (IFA). In addition, a one step RT-PCR was done for the detection of hMPV from the viral RNA extracts. Of the 21 NPA samples tested for respiratory viral antigens by IFA, two were positive for RSV (9.5%), one was positive for influenza A (4.8%) and one was positive for both adenovirus and PIV-2 (4.8%). Of the 21 NPA viral RNA extracts tested by RT-PCR, 18 (86%) were positive for hMPV, in which 2 were co-infected with RSV and influenza A virus, respectively. hMPV was the predominant cause of SARI outbreak (2014) in children presented to the THP, Sri Lanka.

Keywords

Human metapneumovirus Severe acute respiratory infections Children IFA RT-PCR 

Notes

Funding

Grant No. RG/AF/2013/38/M from University of Peradeniya.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval and consent to participation

The study was approved by the Ethical Review Committee of the Faculty of Medicine, University of Peradeniya, Sri Lanka. An informed written consent was obtained from the parents/guardians of the study participants.

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Copyright information

© Indian Virological Society 2019

Authors and Affiliations

  1. 1.Department of Microbiology, Faculty of MedicineUniversity of PeradeniyaPeradeniyaSri Lanka
  2. 2.Department of Paediatrics, Faculty of MedicineUniversity of PeradeniyaPeradeniyaSri Lanka
  3. 3.Teaching HospitalPeradeniyaSri Lanka

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