Molecular characterization of peste-des-petits ruminants virus from Nepal, 2005 to 2016
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Outbreaks of peste-des-petits ruminants (PPR) has been reported regularly in Nepal since 1994. Despite this, there has been limited molecular characterization of circulating virus in the country. In this study a 351 bp segment of the nucleoprotein gene of the PPR virus (PPRV) was amplified and sequenced from ten samples collected between 2005 and 2016. Phylogenetic trees were estimated from these sequences using the maximum likelihood method confirming that all of the PPRV from the samples analysed belonged to the sub-clade IV of clade I of lineage IV and that they shared a common origin with other PPRV isolates in the region.
Keywordspeste-des-petits ruminants Nepal Lineage IV Molecular epidemiology
This study was supported by funds from the IAEA Peaceful Uses Initiative (PUI) VETLAB Network.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests regarding the publication of this study.
Human and animal rights
No animal work was performed for this study.
- 6.Dundon WG, Adombi CM, Kanu S, Loitsch A, Cattoli G, Diallo A. Complete genome sequence of a lineage II peste des petits ruminants virus from Sierra Leone. Genome Announce. 2018;6:e01417–e01417.Google Scholar
- 7.Gargadennec L, Lalanne A. La peste des petits ruminants. Bull Serv Zoo A O F. 1942;5:16–21.Google Scholar
- 9.Kumar KS, Babu A, Sundarapandian G, Roy P, Thangavelu A, Kumar KS, Arumugam R, Chandran ND, Muniraju M, Mahapatra M, Banyard AC, Manohar BM, Parida S. Molecular characterisation of lineage IV peste des petits ruminants virus using multi gene sequence data. Vet Microbiol. 2014;174:39–49.CrossRefGoogle Scholar
- 11.Muthuchelvan D, De A, Debnath B, Choudhary D, Venkatesan G, Rajak KK, Sudhakar SB, Himadri D, Pandey AB, Parida S. Molecular characterization of peste-des-petits ruminants virus (PPRV) isolated from an outbreak in the Indo-Bangladesh border of Tripura state of North-East India. Vet Microbiol. 2014;174:591–5.CrossRefGoogle Scholar