Characterization of Marek’s disease virus and phylogenetic analyses of meq gene from an outbreak in poultry in Meghalaya of Northeast India
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The aim of the present study was to characterize the virus from the lesions and histopathology of organs associated with mortality in Kuroiler (dual purpose variety of poultry developed and marketed by Keggfarms Pvt. Ltd, India) birds suspected of Marek’s disease. Among 1047 birds from two farms of different location with 5.5 and 34% mortality, two types of lesion were observed in post mortem examination; tumors in vital organs—liver, spleen, kidney, lung and ovaries and generalized small nodular tumour in the abdominal cavity. Molecular characterization based on detection of ICP4 gene showed the presence of Marek’s disease virus (MDV) from tissues and cell culture adapted isolates in Madin Darby Canine Kidney cell lines. Histopathological examination revealed multinucleated immature lymphoid cells infiltration in the organs. Phylogenetic analysis of the isolates based on meq gene showed the isolates belongs to cluster I genotype of MDV. This is for the first time the MDV virus is characterized from an outbreak in the poultry flock in farmer’s field affecting production in Meghalaya state of North east India.
KeywordsMarek’s disease Kuroiler Oncogenic virus Serotype Genotype
The authors would like to acknowledge Professor Vandana Raphael, Dean Cum HOD, Deptt of Pathology, North Eastern Indira Gandhi Regional Institute of Health and Medical Sciences, Shillong, Meghalaya and her team for help in preparation of histopathological slides and photographs. The research work is institute project (code IXX11689) of Indian Council of Agriculture Research - Research Complex for North Eastern Hill Region, Umiam, Meghalaya.
- 1.Arulmozhi A, Saravanan S, Mohan B, Balasubramaniam GA. Marek’s disease in vaccinated poultry flocks in and around Namakkal region of Tamil Nadu. Indian J Vet Pathol. 2011;35:45–7.Google Scholar
- 4.Davidson I. Differential diagnosis of avian oncogenic viruses. 2001. http://ww.poultrymed.com. Accessed 6 Oct 2009.
- 5.Davidson I. Veiled and unveiled aspects of infection with oncogenic viruses. Keynote lecture article. In: Proceedings of the 14th world veterinary poultry congress, Istanbul, Turkey. 2005.Google Scholar
- 10.Kalyani HI, Tajpara MM, Jhala MK, Bhanderi BB, Nayak JB, Purohit JH. Characterization of the ICP4 gene in pathogenic Marek’s disease virus of poultry in Gujarat, India, using PCR and sequencing. Veterinarski Arhiv. 2010;80(5):683–92.Google Scholar
- 12.Mohamed MA, Tolba Y, El-Motelib A, Ibrahim AA, El-Deen S, Moustafa E. Contamination rate of Avian Leukosis viruses among commercial Marek’s Disease vaccines in Assiut, Egypt market using Reverse Transcriptase-Polymerase Chain Reaction. Vet World. 2010;3(1):08–12.Google Scholar
- 19.Shamblin CE, Greene N, Arumugaswami V, Dienglewicz RL, Parcells MS. Comparative analysis of Marek’s disease virus (MDV) glycoprotein-, lytic antigen pp38- and transformation antigen Meq-encoding genes: association of meq mutations with MDVs of high virulence. Vet Microbiol. 2004;102:147–67.CrossRefPubMedGoogle Scholar
- 24.Witter RL, Fadly AM. Reticuloendotheliosis. In: Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR, Swayne DE, editors. Diseases of poultry. Ames: Iowa State University Press; 2003. p. 517–35.Google Scholar
- 25.Witter RL, Fadly AM. Reticuloendotheliosis. In: Saif YM, et al., editors. Diseases of poultry. 11th ed. Ames: Iowa State University Press; 2003. p. 517–35.Google Scholar
- 26.Yu ZH, Teng M, Luo J, Wang XW, Ding K, Yu LL, Su JW, Chi JQ, Zhao P, Hu B, Zhang GP, Liu JX. Molecular characteristics and evolutionary analysis of field Marek’s disease virus prevalent in vaccinated chicken flocks in recent years in China. Virus Genes. 2013;47(2):282–91.CrossRefPubMedGoogle Scholar