The evidence of occurrence of porcine circovirus 2 isolation and characterization in Kazakhstan
This report describes the first isolation and characterization of porcine circovirus 2 (PCV2) in the Republic of Kazakhstan. The virus was isolated from a dead piglet that did not exhibit any typical clinical symptoms of porcine circovirus disease at a pig factory in North Kazakhstan oblast (region). The isolated virus belongs to genotype 2 (PCV2) and shares 96.6% sequence homology with one isolate and two strains from China and two French strains in group 1, cluster 1ab and 96.4% homology with two strains isolated in China and one strain from Hungary. Electron microscopy revealed the isolated virus had the typical morphological structure of PCV. This is the evidence of occurrence of porcine circovirus 2 isolation and characterization in Kazakhstan.
KeywordsPCV2 Virus isolation MARC-145 cells Electron microscopy Sequencing Phylogenetic analysis
We are grateful to Professor Zaitsev V.L., research workers Kozhabergenov N.S. and Burashev Ye.D. (Laboratory of Molecular Biology and Genetic Engineering, RIBSP) and researchers Absatova Zh.S. and Kilibayev S.S. (Laboratory Collection of Microorganisms, RIBSP) for their help in implementation of electron microscopy and morphometry of the isolated virus and preparation of the materials for molecular and biological studies. This work was carried out under project 1755/GF4 “Monitoring and genetic characterization of viruses which cause infectious diseases in pigs in Kazakhstan” for 2015–2017, funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan.
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