Advertisement

VirusDisease

, Volume 29, Issue 1, pp 118–122 | Cite as

The evidence of occurrence of porcine circovirus 2 isolation and characterization in Kazakhstan

  • M. Mambetaliyev
  • N. B. Yesimbekova
  • V. M. Strochkov
  • K. K. Tabynov
  • K. T. Sultankulova
  • Ye. O. Abduraimov
Short Communication

Abstract

This report describes the first isolation and characterization of porcine circovirus 2 (PCV2) in the Republic of Kazakhstan. The virus was isolated from a dead piglet that did not exhibit any typical clinical symptoms of porcine circovirus disease at a pig factory in North Kazakhstan oblast (region). The isolated virus belongs to genotype 2 (PCV2) and shares 96.6% sequence homology with one isolate and two strains from China and two French strains in group 1, cluster 1ab and 96.4% homology with two strains isolated in China and one strain from Hungary. Electron microscopy revealed the isolated virus had the typical morphological structure of PCV. This is the evidence of occurrence of porcine circovirus 2 isolation and characterization in Kazakhstan.

Keywords

PCV2 Virus isolation MARC-145 cells Electron microscopy Sequencing Phylogenetic analysis 

Notes

Acknowledgements

We are grateful to Professor Zaitsev V.L., research workers Kozhabergenov N.S. and Burashev Ye.D. (Laboratory of Molecular Biology and Genetic Engineering, RIBSP) and researchers Absatova Zh.S. and Kilibayev S.S. (Laboratory Collection of Microorganisms, RIBSP) for their help in implementation of electron microscopy and morphometry of the isolated virus and preparation of the materials for molecular and biological studies. This work was carried out under project 1755/GF4 “Monitoring and genetic characterization of viruses which cause infectious diseases in pigs in Kazakhstan” for 2015–2017, funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan.

References

  1. 1.
    Allan GM, Ellis JA. Porcine circoviruses: a review. J Vet Diagn Investig. 2000;12:3–14.CrossRefGoogle Scholar
  2. 2.
    Guo LJ, Lu YH, Wei YW, Huang LP, Liu CM. Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China. Virol J. 2010;7:273.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Segalés J, Olvera A, Grau-Roma L, Charreyre C, Nauwynck H, Larsen L, Dupont K, Mc Cullough K, Ellis J, Krakowka S, Mankertz A, Fredholm M, Fossum C, Timmusk S, Stockhofe-Zurwieden N, Beattie V, Armstrong D, Grassland B, Baekbo P, Allan G. PCV-2 genotype definition and nomenclature. Vet Rec. 2008;162:867–8.CrossRefPubMedGoogle Scholar
  4. 4.
    Gerber PF, Johnson J, Shen H, Striegel D, Xiao CT, Halbur PG, Opriessnig T. Association of concurrent porcine circovirus (PCV) 2a and 2b infection with PCV associated disease in vaccinated pigs. Res Vet Sci. 2013;95(2):775–81.CrossRefPubMedGoogle Scholar
  5. 5.
    Nainys J, Lasickiene R, Petraityte-Burneikiene R, Dabrisius J, Lelesius R, Sereika V, Zvirbliene A, Sasnauskas K, Gedvilaite A. Generation in yeast of recombinant virus-like particles of porcine circovirus type 2 capsid protein and their use for a serologic assay and development of monoclonal antibodies. BMC Biotechnol. 2014;14:100.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Allan GM, et al. Infection of leucocyte cell cultures derived from different species with pig circovirus. Vet Microbiol. 1994;41:267–79.CrossRefPubMedGoogle Scholar
  7. 7.
    Wang F, Guo X, Ge X, Wang Z, Chen Y, Cha Z, Yang H. Genetic variation analysis of Chinese strains of porcine circovirus type 2. Virus Res. 2009;145:151–6.CrossRefPubMedGoogle Scholar
  8. 8.
    Sjurin VN, Belousova RV, Fomina NV. Veterinary virology: preparation of a virus-containing material for the study. Moscow: Kolos; 1984. p. 247 (in Russian).Google Scholar
  9. 9.
    Fenaux M, Halbur PG, Gill M, Toth TE, Meng X-J. Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol. 2000;38(7):2494–503.PubMedPubMedCentralGoogle Scholar
  10. 10.
    Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980;16:111–20.CrossRefPubMedGoogle Scholar
  11. 11.
    Opriessnig T. The value of genetic structure of porcine circovirus of type 2 and its monitoring. http://www.pig333.ru/porcine_circovirus (in Russian). Accessed 7 July 2017

Copyright information

© Indian Virological Society 2018

Authors and Affiliations

  1. 1.Research Institute for Biological Safety Problems (RIBSP), Science CommitteeMinistry of Education and Science of the Republic of KazakhstanKordaiskiy rayon, GvardeyskiyKazakhstan

Personalised recommendations