Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya
- 62 Downloads
The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure.
Keywords5′UTR CSFV E2 I-TASSER RT-PCR
This work was supported by a grant from Department of Biotechnology, Government of India, (DBT-NER/LIVS/11/2012dated24-04-2014, Project ‘‘Advance Animal Disease Diagnosis and Management Consortium’’). We thank all the persons involved in collection of samples from various farms.
- 4.Bett B, Deka R, Padmakumar V, Rajasekhar M. Classical swine fever in northeast India: Prevention and control measures. Policy Brief. Nairobi: International Livestock Research Institute; 2012.Google Scholar
- 5.Chakraborty SV, Chakraborty S, Veeregoda B, Rathnamma D, Venkatesha M, Leena G, Veeresh H, Patil S. Molecular characterization and genogrouping of classical swine fever virus isolated from field outbreaks. Indian J Anim Sci. 2011;81:8.Google Scholar
- 11.Holmes EC. Evolution and emergence of RNA viruses. Oxford: Oxford University Press; 2009.Google Scholar
- 30.Van Regenmortel MH, Fauquet C, Bishop D, Carstens E, Estes M, Lemon S, Maniloff J, Mayo M, McGeoch D, Pringle C. Virus taxonomy: classification and nomenclature of viruses. In: Seventh report of the international committee on taxonomy of viruses. Academic Press. 2000.Google Scholar