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Molecular evidence of an isolate of mungbean yellow mosaic India virus with a recombinant DNA B component occurring on mungbean from mid-hills of Meghalaya, India

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Abstract

A new isolate (Mg-mungbean-1) of yellow mosaic virus (YMV) was identified and characterized from mungbean growing in mid-hill condition of Meghalaya, India. Full genome of components (DNA A and DNA B; NCBI accessions number KU95030 and KU95031, respectively) of the virus were amplified through rolling circle amplification and sequenced. Both, DNA A and DNA B shared a common region (CR) with 90.4% similarity. The DNA A of Mg-mungbean-1 showed maximum (97.59%) nucleotide identity with mungbean yellow mosaic India virus (MYMIV) isolate (HF922628) reported from West Bengal, India and DNA B showed ~ 96% nucleotide identity with mungbean yellow mosaic virus (MYMV) isolates having variant DNA B. Phylogenetic tree of DNA A also identified Mg-mungbean-1 as a MYMIV. Based on DNA B the current isolate grouped with the variant Indian MYMV isolates. One recombination event in the CR of DNA B of Mg-mungbean-1 was detected, where MYMV:India:clonePB1 and MYMIV:India:cloneMBB-B31 have been identified as major and minor parents, respectively. Overall, the current study indicated occurrence of an isolate of MYMIV with a recombinant DNA B component on mungbean from mid-hills of Meghalaya, India. To the best of our knowledge this is the first molecular characterization of YMV from northeast India.

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Acknowledgements

This work was supported by the Department of Biotechnology, Government of India [grant number BT/324/NE/TBP/2012].

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  1. Somnath Roy

    Present address: ICAR-National Rice Research Institute, Central Rainfed Upland Rice Research Station, Hazaribag, Jharkhand, 825 301, India

Authors and Affiliations

  1. ICAR Research Complex for NEH Region, Umiam, Meghalaya, 793 103, India

    Amrita Banerjee

  2. College of Post Graduate Studies, Central Agricultural University, Umiam, Meghalaya, 793 103, India

    Yashi Umbrey

  3. ICAR-National Bureau of Plant Genetic Resources, Regional Station, Umiam, Meghalaya, 793 103, India

    Rohit M. Yadav & Somnath Roy

  4. ICAR-National Rice Research Institute, Central Rainfed Upland Rice Research Station, Hazaribag, Jharkhand, 825 301, India

    Amrita Banerjee

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  1. Amrita Banerjee
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Correspondence to Amrita Banerjee.

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Banerjee, A., Umbrey, Y., Yadav, R.M. et al. Molecular evidence of an isolate of mungbean yellow mosaic India virus with a recombinant DNA B component occurring on mungbean from mid-hills of Meghalaya, India. VirusDis. 29, 68–74 (2018). https://doi.org/10.1007/s13337-018-0429-5

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