Canine parvovirus types 2a and 2c detection from dogs with suspected parvoviral enteritis in Peru
Canine parvovirus type 2 (CPV-2) has been reported worldwide as the main agent related to acute hemorrhagic enteritis of high morbidity and variable mortality in puppies. The detection and characterization of this virus is essential to understand the etiology of the disease and to develop control measures. To characterize the virus circulating in Peruvian dogs and to provide new insights into the local diversity of CPV-2, rectal swabs from 39 puppies with clinical symptoms and with no history of previous vaccinations were analyzed. Total DNA was extracted by fast boiling method, and PCR and sequencing were performed using specific primers that amplify a 1316 bp fragment corresponding to the VP2 gene of CPV-2. CPV-2 was detected in 62% of the analyzed samples. The sequencing of PCR product was possible in 9 samples, which were identified as type 2a (4 samples) and type 2c (5 samples). A phylogenetic analysis of both variants circulating in Peruvian dogs showed similarities to Equatorian and Uruguayan strains. This work constitutes the first report about genetic characterization of CPV-2 in Peru.
KeywordsCanine parvovirus VP2 PCR Antigenic variant
We are grateful to Dr. Rubén Pérez for valuable suggestions about CPV-2 research. We thanks to doctors Efraín Gonzales, Tito Gonzalez and César Acuña from the participating veterinary clinics for generously facilitating the extraction of samples.
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