In 2016, begomovirus-like symptoms were observed in mungbean (Vigna radiata) and black gram (Vigna mungo) in Pakistan. Symptomatic and asymptomatic leaf samples were collected and total genomic DNA was extracted. Full length begomovirus and betasatellite genomes were PCR amplified and cloned in plasmid pTZ/57RT cloning vector. The cloned molecules were subjected to Sanger sequencing. Based on Sequence Demarcation Tool, the begomovirus clone SR-3 identified in mungbean showed 94.50% highest identity with cotton leaf curl Kokhran virus-Kokhran (CLCuKoV-Ko), while the clone SR-5 isolated from black gram showed 99.7% highest identity with CLCuKoV-Ko. In phylogenetic tree analysis the identified isolates made a lower branch tree with CLCuKoV-Ko. The betasatellite clone SR-79 isolated from black gram showed 97% similarity with chili leaf curl betasatellite (ChLCB) and the betasatellite clone SR-93 isolated from mungbean showed 97.7% similarity with ChLCB. Southern blot analysis also confirmed the presence of CLCuKoV-Ko. This is the first report of CLCuKoV-Ko associated with ChLCB infecting both mungbean and black gram in Pakistan. This presents the new combination of disease complex and increase in host range of this virus species, which shows a real threat to other crops especially legume crops in Pakistan.
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Azhar MT, Amin I, Anjum ZI, Arshad M, Briddon RW, Mansoor S (2010) Both malvaceous and non-malvaceous betasatellites are associated with two wild cotton species grown under field conditions in Pakistan. Virus Genes 41(3):417–424
Briddon R, Markham P (1994) Universal primers for the PCR amplification of dicot-lnfecting geminiviruses. Mol Biotechnol 1(2):202–205
Briddon RW, Bull SE, Mansoor S, Amin I, Markham P (2002) Universal primers for the PCR-mediated amplification of DNA β. Mol Biotechnol 20(3):315–318
Bull S, Briddon R, Markham P (2003) Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA β complexes. Mol Biotechnol 23(1):83–86
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Fondong VN (2013) Geminivirus protein structure and function. Mol Plant Pathol 14(6):635–649
Foyer CH, Lam H-M, Nguyen HT, Siddique KH, Varshney RK, Colmer TD, Cowling W, Bramley H, Mori TA, Hodgson JM (2016) Neglecting legumes has compromised human health and sustainable food production. Nat Plants 2:16112
Hanley-Bowdoin L, Bejarano ER, Robertson D, Mansoor S (2013) Geminiviruses: masters at redirecting and reprogramming plant processes. Nat Rev Microbiol 11(11):777–788
Hema M, Sreenivasulu P, Patil BL, Kumar PL, Reddy D (2014) Tropical food legumes: virus diseases of economic importance and their control. Adv Virus Res 90:431–505
Hussain M, Iram S, Mansoor S, Briddon RW (2009) A single species of betasatellite is prevalent in chilli across north central Pakistan and shows phylogeographic segregation. J Phytopathol 157(9):576–579
Ilyas M, Qazi J, Mansoor S, Briddon RW (2009) Molecular characterisation and infectivity of a “Legumovirus”(genus Begomovirus: family Geminiviridae) infecting the leguminous weed Rhynchosia minima in Pakistan. Virus Res 145(2):279–284
Inoue-Nagata AK, Lima MF, Gilbertson RL (2016) A review of geminivirus diseases in vegetables and other crops in Brazil: current status and approaches for management. Hortic Bras 34(1):8–18
Jamil N, Rehman A, Hamza M, Hafeez A, Ismail H, Zubair M, Mansoor S, Amin I (2017) First report of Tomato leaf curl New Delhi virus, a bipartite begomovirus, infecting soybean (Glycine max). Plant Dis 101(5):845–845
Li F, Xu X, Huang C, Gu Z, Cao L, Hu T, Ding M, Li Z, Zhou X (2015) The AC5 protein encoded by Mungbean yellow mosaic India virus is a pathogenicity determinant that suppresses RNA silencing-based antiviral defenses. New Phytol 208(2):555–569
Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 1(1):1–5
Muhire BM, Varsani A, Martin DP (2014) SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PloS One 9(9):1–8
Patil BL, Bagewadi B, Yadav JS, Fauquet CM (2016) Mapping and identification of cassava mosaic geminivirus DNA-A and DNA-B genome sequences for efficient siRNA expression and RNAi based virus resistance by transient agro-infiltration studies. Virus Res 213:109–115
Qazi J, Ilyas M, Mansoor S, Briddon RW (2007) Legume yellow mosaic viruses: genetically isolated begomoviruses. Mol Plant Pathol 8(4):343–348
Rahman SU, Zubair M, Ahmed N, Khan MZ, Raza G, Amin I, Mansoor S (2019) Identification of “Malvastrum yellow vein Lahore virus” a proposed new species of begomovirus in association with cotton leaf curl Multan betasatellite infecting green bean (Phaseolus vulgaris) in Pakistan. Australas Plant Dis Notes 14(1):38
Rahman SU, Zubair M, Ahmed N, Khan MZ, Raza G, Amin I, Mansoor S (2020) First report of pepper leaf curl Bangladesh virus (PepLCBV) associated with cotton leaf curl Multan betasatellite on kidney bean (Phaseolus vulgaris) in Pakistan. J Plant Pathol:1–2
Raj S, Khan M, Snehi S, Srivastava S, Singh H (2006) A yellow mosaic disease of soybean in northern India is caused by Cotton leaf curl Kokhran virus. Plant Dis 90(7):975–975
Rey ME, Ndunguru J, Berrie LC, Paximadis M, Berry S, Cossa N, Nuaila VN, Mabasa KG, Abraham N, Rybicki EP (2012) Diversity of dicotyledenous-infecting geminiviruses and their associated DNA molecules in Southern Africa, including the South-West Indian Ocean Islands. Viruses 4(9):1753–1791
Roman GV, Epure LI, Toader M, Lombardi A-R (2016) Grain legumes-main source of vegetal proteins for European consumption. Agrolife Sci J 5(1):178–183
Sattar MN, Kvarnheden A, Saeed M, Briddon RW (2013) Cotton leaf curl disease–an emerging threat to cotton production worldwide. J Gen Virol 94(4):695–710
Tahir MN, Mansoor S (2011) βC1 of chili leaf curl betasatellite is a pathogenicity determinant. Virol J 8(1):509
Tahir M, Amin I, Briddon R, Mansoor S (2011) The merging of two dynasties—Identification of an african cotton leaf curl disease-associated. PloS One 6(5):1–9
Zubair M, Zaidi SS-e-A, Shakir S, Farooq M, Amin I, Scheffler JA, Scheffler BE, Mansoor S (2017) Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton. Sci Rep 7(1):680
The study was in part supported by the “Pak-US cotton productivity enhancement program” of the International Center for Agricultural Research in the Dry Areas (ICARDA) funded by United States Department of Agriculture (USDA), Agricultural Research Service (ARS), under agreement no. 58-6402-0-178F. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or ICARDA.
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Rahman, S.U., Zubair, M., Ahmed, N. et al. Cotton leaf curl Kokhran virus in association with Chili leaf curl betasatellite infecting mungbean (Vigna radiata.) and black gram (Vigna mungo.) in Pakistan. Australasian Plant Pathol. 49, 461–465 (2020). https://doi.org/10.1007/s13313-020-00719-3
- Black gram
- Cotton leaf curl Kokhran virus
- Chili leaf curl betasatellite