Abstract
In 2016, begomovirus-like symptoms were observed in mungbean (Vigna radiata) and black gram (Vigna mungo) in Pakistan. Symptomatic and asymptomatic leaf samples were collected and total genomic DNA was extracted. Full length begomovirus and betasatellite genomes were PCR amplified and cloned in plasmid pTZ/57RT cloning vector. The cloned molecules were subjected to Sanger sequencing. Based on Sequence Demarcation Tool, the begomovirus clone SR-3 identified in mungbean showed 94.50% highest identity with cotton leaf curl Kokhran virus-Kokhran (CLCuKoV-Ko), while the clone SR-5 isolated from black gram showed 99.7% highest identity with CLCuKoV-Ko. In phylogenetic tree analysis the identified isolates made a lower branch tree with CLCuKoV-Ko. The betasatellite clone SR-79 isolated from black gram showed 97% similarity with chili leaf curl betasatellite (ChLCB) and the betasatellite clone SR-93 isolated from mungbean showed 97.7% similarity with ChLCB. Southern blot analysis also confirmed the presence of CLCuKoV-Ko. This is the first report of CLCuKoV-Ko associated with ChLCB infecting both mungbean and black gram in Pakistan. This presents the new combination of disease complex and increase in host range of this virus species, which shows a real threat to other crops especially legume crops in Pakistan.
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Acknowledgements
The study was in part supported by the “Pak-US cotton productivity enhancement program” of the International Center for Agricultural Research in the Dry Areas (ICARDA) funded by United States Department of Agriculture (USDA), Agricultural Research Service (ARS), under agreement no. 58-6402-0-178F. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or ICARDA.
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SM and IA gave the basic idea of studying the genetic diversity of begomovirus in legume crops. SR wrote the manuscript. SR and MZ conducted the field sampling and experiment. SM, GR, NA, MZK and IA helped in the bioinformatics analysis and critically reviewed the manuscript.
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Rahman, S.U., Zubair, M., Ahmed, N. et al. Cotton leaf curl Kokhran virus in association with Chili leaf curl betasatellite infecting mungbean (Vigna radiata.) and black gram (Vigna mungo.) in Pakistan. Australasian Plant Pathol. 49, 461–465 (2020). https://doi.org/10.1007/s13313-020-00719-3
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DOI: https://doi.org/10.1007/s13313-020-00719-3