Australasian Plant Pathology

, Volume 47, Issue 2, pp 163–170 | Cite as

Microsatellite analysis of Puccinia triticina from Triticum and Aegilops hosts

  • Elena I. Gultyaeva
  • Ekaterina L. Shaydayuk
  • Igor’ A. Kazartsev
  • Aigul Аkhmetova
  • Evsey Kosman
Original Paper


Genetic variation between different populations of Puccinia triticina from Russia and Kazakhstan was evaluated, based on 18 simple sequence repeat (SSR) markers. Seventeen collections of P. triticina (181 single-uredinial isolates, in total) obtained from 15 different host species belonging to Aegilops and Triticum were included in the study. All single-uredinial isolates were obtained from naturally infected plants in experimental fields in Dagestan (Caucasus region of Russia), Novosibirsk (West Siberian region of Russia) and Shortandy (Northern Kazakhstan). Regional differentiation of P. triticina SSR genotypes was detected. Collections from host plants with the same ploidy and/or genetically similar background were genetically similar. Collections from the hexaploid species in Dagestan were highly similar to those from common wheat. Collections from tetraploid species T. aethiopiсum, T. turaniсum, T. dicoccum (genome BВAuAu) from Dagestan were moderately different from the collections originated from the hexaploid species. Puccinia triticina from Ae. crassa (DcDcMM) collected in Dagestan was genetically similar to populations from common wheat and other hexaploid species. Collections from Ae. tauschii (2n = 2× = 14) were significantly different from those obtained from tetraploid (2n = 4× =28) and hexaploid (2n = 6× =42) host species collected on the experimental field in Dagestan. SSR genotypes of P. triticina isolates from T. durum in Kazakhstan were highly dissimilar to those sampled from all other hosts. A low association was established between the SSR genotypes of P. triticina identified in the present study and the virulence phenotypes for the same set of collections, as observed in a previous study.


Aegilops spp. Infinite alleles model Microsatellite markers Puccinia triticina Triticum spp. Wheat leaf rust 



This study was supported by Russian Scientific Foundation (RSF) (project №14-26-00067).


  1. Berlyand-Kozhevnikov VM, Dmitriev AP, Budashkina EB, Shitova IT, Reiter BG (1978) Wheat resistance to leaf rust (genetic diversity of fungus populations and plant-host). Nauka, Novosibirsk in RussGoogle Scholar
  2. Boguslavskii RL, Golik OV (2003) Aegilops L. species as genetic recourse of breeding. Kharkov (in Russ) 160 p. Book in Russian, The Рlant Production Institute nd. a. V. Ya. Yuryev of NAASGoogle Scholar
  3. Dorofeev DF, Udachin RA, Semenova LV, Novikova MV, Gradchaninova OD, Shitova IP, Merezhko AF, Filatenko AA (1987) Wheat of world. Kolos, Leningrad in RussGoogle Scholar
  4. Duan X, Enjalbert J, Vautrin D, Solignac C, Giraut T (2003) Isolation of 12 microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia triticina. Mol Ecol 3(1):65–67. CrossRefGoogle Scholar
  5. Goncharov NP (2012) Comparative genetics of wheats and their related species. In Shumny VK (ed) 2nd edn. Academic Publishing House “GEO“, Novosibirsk (in Russian with English summary)Google Scholar
  6. Goyeau H, Halkett F, Zapater MF, Carlier J, Lannou C (2007) Clonality and host selection in the wheat pathogenic fungus Puccinia triticina. Fungal Genet Biol 44(6):474–483. CrossRefPubMedGoogle Scholar
  7. Gultyaeva EI, Shaydayuk EL, Goncharov NP, Аkhmetova A, Abdullaev KM, Belousova MH, Kosman E (2016) Virulence of Puccinia triticina on Triticum and Aegilops species. Australas Plant Pathol 45(2):155–163. CrossRefGoogle Scholar
  8. Gultyaeva EI, Aristova MK, Shaidayuk EL, Mironenko NV, Kazartsev IA, Akhmetova A, Kosman E (2017) Genetic differentiation of Puccinia triticina Erikss. In Russia. Russ J Genet 53(9):998–1005. CrossRefGoogle Scholar
  9. Justesen AF, Ridout CJ, Hovmøller MS (2002) The recent history of Puccinia striiformis f. Sp. tritici in Denmark as revealed by disease incidence and AFLP markers. Plant Pathol 51(1):13–23. CrossRefGoogle Scholar
  10. Kolmer JA, Liu JQ (2000) Virulence and molecular polymorphism in international collections of the wheat leaf rust fungus Puccinia triticina. Phytopathology 90(4):427–436. CrossRefPubMedGoogle Scholar
  11. Kolmer JA, Liu JQ, Siem M (1995) Virulence and molecular polymorphism in Puccinia recondita f. Sp. tritici in Canada. Phytopathology 85: 276–285Google Scholar
  12. Kolmer JA, Hanzalova A, Goyeau H, Bayles R, Morgounov A (2013) Genetic differentiation of the wheat leaf rust fungus Puccinia triticina in Europe. Plant Pathol 62(1):21–31. CrossRefGoogle Scholar
  13. Kosman E (1996) Difference and diversity of plant pathogen populations: a new approach for measuring. Phytopathology 86:1152–1155Google Scholar
  14. Kosman E (2014) Measuring diversity: from individuals to populations. Eur J Plant Pathol 138(3):467–486. CrossRefGoogle Scholar
  15. Kosman E, Leonard KJ (2005) Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Mol Ecol 14(2):415–424. CrossRefPubMedGoogle Scholar
  16. Kosman E, Leonard KJ (2007) Conceptual analysis of methods applied to assessment of diversity within and distance between populations with asexual or mixed mode of reproduction. New Phytol 174(3):683–696. CrossRefPubMedGoogle Scholar
  17. Liu M, Rodrigue N, Kolmer J (2014) Population divergence in the wheat leaf rust fungus Puccinia triticina is correlated with wheat evolution. Heredity 112(4):443–453. CrossRefPubMedGoogle Scholar
  18. Long DL, Kolmer JA (1989) A north American system of nomenclature for Puccinia recondita f.Sp. tritici. Phytopathology 79(5):525–529. CrossRefGoogle Scholar
  19. Mantovani P, Maccaferri M, Tuberosa R, Kolmer J (2010) Virulence phenotypes and molecular genotypes in collections of Puccinia triticina from Italy. J Plant Dis Protection 94:420–424Google Scholar
  20. Mikhailova LA (1973) Population and genetic research of wheat leaf rust (Puccinia recondita Rob. ex Desm. f. sp. tritici) in Dagestan. Dissertation, Leningrad in RussGoogle Scholar
  21. Mikhailova LA, Gultyaeva EI, Mironenko NV (2000) Research methods for populations structure of the activator of wheat leaf rust Puccinia recondita rob. Ex Desm. f. Sp. tritici. Immunogenetic methods of creation of cultivars resistant to harnful organisms, Sankt- Peterburg in RussGoogle Scholar
  22. Ordoñez ME, Kolmer JA (2007) Simple sequence repeat diversity of a world-wide collection of Puccinia triticina from durum wheat. Phytopathology 97(5):574–583. CrossRefPubMedGoogle Scholar
  23. Ordonez ME, German SE, Kolmer JA (2010) Genetic differentiation within the Puccinia triticina population in South America and comparison with the north American population suggests common ancestry and intercontinental migration. Phytopathology 100(4):376–383. CrossRefPubMedGoogle Scholar
  24. Park RF, Jahoor A, Felsenstein FG (2000) Population structure of Puccinia recondita in Western Europe during 1995 as assessed by variability in pathogenicity and molecular markers. Phytopathology 148(3):169–179. CrossRefGoogle Scholar
  25. Schachtel GA, Dinoor A, Herrmann A, Kosman E (2012) Comprehensive evaluation of virulence and resistance data: a new analysis tool. J Plant Dis Protection 96:1060–1063Google Scholar
  26. Szabo LS, Kolmer JA (2007) Development of simple sequence repeat markers for the plant pathogenic rust fungus Puccinia triticina. Mol Ecol 7(4):708–710. CrossRefGoogle Scholar
  27. Wang X, Mulock B, Bakkeren G, McCallum B (2010a) Development of EST-derived simple sequence repeat markers for wheat leaf rust fungus, Puccinia triticina Eriks. Can J Plant Pathol 32(1):98–107. CrossRefGoogle Scholar
  28. Wang X, Bakkeren G, McCallum B (2010b) Virulence and molecular polymorphisms of the wheat leaf rust fungus Puccinia triticina in Canada from 1997 to 2007. Botany 88(6):575–589. CrossRefGoogle Scholar
  29. Wu JQ, Sakthikumar S, Dong C, Zhang P, Cuomo CA, Park RF (2017) Comparative genomics integrated with association analysis identifies candidate effector genes corresponding to Lr20 in phenotype-paired Puccinia triticina isolates from Australia. Front Plant Sci 8:148PubMedPubMedCentralGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2018

Authors and Affiliations

  • Elena I. Gultyaeva
    • 1
  • Ekaterina L. Shaydayuk
    • 1
  • Igor’ A. Kazartsev
    • 1
  • Aigul Аkhmetova
    • 2
  • Evsey Kosman
    • 3
  1. 1.All-Russian Institute of Plant ProtectionSt. Petersburg-PushkinRussia
  2. 2.A.I. Barayev Research and Production Center of Grain FarmingShortandyKazakhstan
  3. 3.Institute for Cereal Crops ImprovementTel Aviv UniversityTel AvivIsrael

Personalised recommendations