Evaluation of microsatellite primers developed for grapevine downy mildew, Plasmopara viticola, on Australian isolates
Grapevine downy mildew (Plasmopara viticola) is a serious economic disease for Australian viticulture. Using different detection techniques 51 new and revised microsatellite primers have been developed for P. viticola based on American and European isolates. Thus far, only a subset of the developed microsatellites have been tested for polymorphisms among Australian P. viticola populations. This study tests all 51 microsatellites, under the same PCR conditions, for polymorphism on Australian isolates from geographically distinct regions. Of the 51 microsatellites, 18 showed polymorphism between the isolates tested and or P. viticola GenBank sequences. A selection of these microsatellites will be used for a larger population analysis study on Australian isolates.
KeywordsPlasmopara viticola Downy mildew Grapevines Microsatellite Population genetics
This work was supported by an Australian Government Research Training Program Scholarship and Wine Australia (GWR Ph1301).
- CABI (2017) Plasmopara viticola. In: Crop protection compendium. CAB International, Wallingford. www.cabi.org/cpc
- de Castella F, Brittlebank CC (1917) Notes on downy mildew (Plasmopara viticola, B. and de T.) J Agric (Vic) 15:685–700Google Scholar
- Delmotte F, Chen WJ, Richard-Cervera S, Greif C, Papura D, Giresse X, Mondor-Genson G, Corio-Costet MF (2006) Microsatellite DNA markers for Plasmopara viticola, the causal agent of downy mildew of grapes. Mol Ecol Notes 6(2):379–381. https://doi.org/10.1111/j.1471-8286.2005.01240.x CrossRefGoogle Scholar
- Emmett R, Harris A, Taylor R, McGechan J (1992) Grape disease and vineyard protection. In: Coombe B, Dry P (eds) Viticulture, vol II, Practices. Winetitles, Adelaide, pp 232–278Google Scholar
- Hug F (2005) Genetic structure and epidemiology of Plasmopara viticola populations from Australian grape growing regions. ETH Zurich, Diploma thesisGoogle Scholar
- Kearse M, Moir R, Wilson A, Stone-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12):1647–1649. https://doi.org/10.1093/bioinformatics/bts199 CrossRefPubMedPubMedCentralGoogle Scholar
- Mclean G, Magarey P, Wachtel M, Dry P (1984) A climatic evaluation of the question: could grapevine downy mildew develop in Western Australia. In: Lee T, Somers T (eds) Advances in viticulture oenology for economic gain. Proceedings of the fifth Australian wine industry conference, 29 November - 1 December 1983, Perth Western Australia, 1984. The Australian Wine Research Institute, pp 249–260Google Scholar
- Rouxel M, Papura D, Nogueira M, Machefer V, Dezette D, Richard-Cervera S, Carrere S, Mestre P, Delmotte F (2012) Microsatellite markers for characterization of native and introduced populations of Plasmopara viticola, the causal agent of grapevine downy mildew. Appl Environ Microbiol 78(17):6337–6340. https://doi.org/10.1128/AEM.01255-12 CrossRefPubMedPubMedCentralGoogle Scholar
- Sakalidis M (2011) Investigation and analysis of taxonomic irregularities with the Botryosphaeriaceae. Murdoch University, PhD thesisGoogle Scholar
- Scholefield P, Morison J (2010) Assessment of economic cost of endemic pests and diseases on the Australian grape and wine industry. Grape and Wine Research and Development Corporation, AdelaideGoogle Scholar
- Wilcox WF, Gubler WD, Uyemoto JK (2015) Compendium of grape diseases, disorders, and pests. The American Phytopathological Society, St PaulGoogle Scholar
- Williams MG (2005) Impact of environmental conditions on the infection behaviour of Western Australian strains of Plasmopara viticola, causal agent of downy mildew in grapevines. University of Western Australia, PhD thesisGoogle Scholar